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. 2019 Mar 22;9:5028. doi: 10.1038/s41598-019-41377-w

Evidence of mitochondrial DNA in the chloroplast genome of Convallaria keiskei and its subsequent evolution in the Asparagales

Gurusamy Raman 1, Seongjun Park 1, Eun Mi Lee 1, SeonJoo Park 1,✉
PMCID: PMC6430787  PMID: 30903007

Abstract

DNA transfer between internal organelles such as the nucleus, mitochondrion, and plastid is a well-known phenomenon in plant evolution, and DNA transfer from the plastid and mitochondrion to the nucleus, from the plastid to the mitochondrion, and from the nucleus to the mitochondrion has been well-documented in angiosperms. However, evidence of the transfer of mitochondrial DNA (mtDNA) to the plastid has only been found in three dicotyledons and one monocotyledon. In the present study, we characterised and analysed two chloroplast (cp) genome sequences of Convallaria keiskei and Liriope spicata, and found that C. keiskei has the largest cp genome (162,109 bp) in the Asparagaceae. Interestingly, C. keiskei had a ~3.3-kb segment of mtDNA in its cp genome and showed similarity with the mt gene rpl10 as a pseudogene. Further analyses revealed that mtDNA transfer only occurred in C. keiskei in the Nolinoideae, which diverged very recently (7.68 million years ago (mya); 95% highest posterior density (HPD): 14.55–2.97 mya). These findings indicate that the C. keiskei cp genome is unique amongst monocotyledon land plants, but further work is necessary to understand the direction and mechanism involved in the uptake of mtDNA by the plastid genome of C. keiskei.

Introduction

The engulfment of bacterial endosymbionts led to the development of eukaryotic cells, and the consequent gradual conversion of those bacteria into eukaryotic organelles such as the mitochondrion and chloroplast (cp)1,2. During this process, there was a significant transfer of cp and mitochondrial (mt) genes from the endosymbiont genomes into the nuclear genome of the host cell3. Lateral and horizontal genetic material transfer between organisms and intracellular gene transfer (IGT) between genomes within organisms are common processes in both prokaryotes and eukaryotes rather than by vertical transfer through sexual reproduction4–8. In plants, the intracellular transfer of genetic material between the cp, mt and nuclear genomes is a common process. The IGT of plastid DNA to the mt and nuclear genomes and the transfer of plastid and mtDNA to the nuclear genome are well-documented and regular phenomena in the land plants4–8. Also, previous studies reported that nuclear DNA has been transferred to mt genomes in the Fabaceae and Cucurbitaceae due to the presence of a permeable transition pore complex in the mitochondria9,10. Nevertheless, land plant plastomes are highly conserved and considered essentially immune to IGT, and it is thought that plant cp genomes do not accept the incorporation of foreign DNA because of the integrity of the plastid membrane7,8,11. However, four studies have documented mt gene transfer to plastomes. In angiosperms, the mt pseudogene cox1 has been transferred into the plastome of Daucus carota11–13, rpl2 was transferred in the common milkweed14, a complete copy of ccmB was transferred in Anacardium occidentale15, and intergenic sequences were transferred in herbaceous bamboos16. In contrast, no evidence of nuclear DNA transfer into the plastid has been reported in any land plant.

In the present study, we characterised and analysed the complete cp genome sequences of two monocotyledon angiosperm plants, Convallaria keiskei Miq. and Liriope spicata (Thunb.) Lour., and conducted comparative genomics of closely related species in the subfamily Nolinoideae and family Asparagaceae, which revealed that C. keiskei has the largest cp genome in the Asparagaceae. We also analysed cp pseudogenes in the Asparagales in order to understand the evolutionary histories of these genes. Furthermore, we identified the transfer of mtDNA, including the rpl10 pseudogene, into the plastome of C. keiskei based on the full genome sequence of the plastome, and confirmed it using gene-specific primers. Additional work confirmed that mtDNA transfer only occurred in the C. keiskei cp genome of the Nolinoideae. Finally, molecular evolutionary analyses suggested that C. keiskei recently diverged. To the best of our knowledge, this is the largest mtDNA segment that has been transferred into the cp genome of the monocotyledon C. keiskei.

Results and Discussion

General features

The plastid genomes of C. keiskei and L. spicata are circular molecules that are 162,109 and 157,055 bp in length, respectively. Both cp genomes exhibit a typical quadripartite structure: the large and small single-copy (LSC and SSC, respectively) regions of C. keiskei are 85,344 and 18,487 bp long, respectively, and there are two parts of an inverted repeat (IR) that are each 29,139 bp long. L. spicata has a 85,374 bp LSC, a 18,727 bp SSC and a 26,477 bp IR (Supplementary Fig. S1). A total of 136 genes were predicted in both genomes, 115 of which were unique and included 81 protein-coding genes, 30 transfer RNA (tRNA) genes, and 4 ribosomal RNA (rRNA) genes (Supplementary Table S1). Nine protein-coding, eight tRNA, and four rRNA genes were duplicated in the IR regions; however, only a part of the ycf1 gene was duplicated in the junction of the SSC and IR regions. A total of 17 intron-containing genes (12 protein-coding genes and 5 tRNAs) were present in both cp genomes. The predicted genes were divided into four categories based on their functions. The first category contained 34 genes, including rRNA and tRNA genes; the second category contained 48 genes that were associated with photosynthesis, including subunits of photosystem I and II, photosynthetic electron-transport-chain-component genes, the rubisco large subunit gene, and presumed NAD(P)H dehydrogenase subunit genes; the third category contained genes that were associated with transcription and translation; and the fourth category contained genes related to amino acids, fatty acids, other biosynthesis-related genes, and some genes with unknown functions (Supplementary Table S2). Although the presence and order of the genes in both genomes were similar to those in other Asparagales species, cp genome size in the Nolinoideae varied from 153 to 162 kb (Fig. 1). The average genome size of the Asparagaceae was approximately 157 kb, but the Polygonatum cyrtonema cp genome was only 153.5 kb long. The cp genome size of C. keiskei was 162 kb, indicating that cp genome size is not highly conserved in the Nolinoideae. C. keiskei has the largest plastome in the Asparagaceae, and the third-largest in the Asparagales behind species in the family Orchidaceae, Cypripedium formosanum and Cypripedium japonicum.

Figure 1.

Figure 1

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Comparison of the large single-copy (LSC), small single-copy (SSC) and inverted repeat (IR) border regions of Asparagaceae chloroplast genomes. Ѱ indicates a pseudogene. The figure is not drawn to scale.

Comparative analysis

The LSC/IRB/SSC/IRA boundary regions of the Nolinoideae and those of the closely related subfamilies Asparagoideae and Lomandroideae were compared. Genes in the boundary regions were highly conserved, with small variations in the cp genomes of the Nolinoideae, except for P. cyrtonema. The LSC region in P. cyrtonema and Eustrephus latifolius is ~82 kb long17. The reduction of ~3 kb was caused by the deletion of nucleotides in the intergenic regions of the LSC. Interestingly, the plastomes of the Lomandroideae varied with SSC and IR region size. The ~2 kb SSC region in the Cordyline indivisa plastome increased due to a complete shift of ycf1 from the IR region. However, in E. latifolius, this was only ~13.6 kb long because ycf1 was in the IR regions, which increased the IR size to ~31.6 kb. The IR region in Convallaria keiskei increased by ~3 kb due to the insertion of mtDNA into its plastome (Fig. 1). Sizes of the LSC, SSC and IR regions were analysed in 23 Asparagaceae cp genomes in order to understand the diversity of the family (Fig. 2). Typical sizes of the LSC, SSC and IR regions in most of the Asparagaceae were ~85, ~18 and ~26 kb, respectively. The size of the LSC region varied from 82,403 to 86,356 bp (Fig. 2a) because of the presence of indels. The SSC region varied from 13,607 to 20,183 bp in length (Fig. 2b) and the IR regions varied from 25,031 to 31,863 bp in length (Fig. 2c), indicating the presence of an indel of the ycf1 gene in both the SSC and IR regions in the plastomes of the Asparagaceae. When compared with another subfamily of the Asparagaceae, the sizes of the LSC, SSC and IR regions of the Lomandroideae and Nolinoideae were extremely diverse, which reflects genome size variation and suggests that these plastomes are not highly conserved. There was a close association between LSC length and genome size, whereas associations between SSC and IR lengths and genome size were highly variable.

Figure 2.

Figure 2

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Relationships between Asparagales chloroplast genome sizes and large single-copy (LSC), small single-copy (SSC) and inverted repeat (IR) region lengths.

Comparative analysis of the pseudogene infA with other Asparagales cp genomes

All of the photosynthetic- and transcriptional-related genes were present in the plastomes of both species, as in other Asparagales. However, some differences were observed in the protein-coding genes of the Asparagales. mVISTA was used to study sequence variations in the Nolinoideae subfamily and other Asparagales plastomes, which revealed that both coding and non-coding regions are not highly conserved in Asparagales plastomes (Supplementary Fig. S2). Specifically, large differences were found in the protein-coding and intergenic regions of the LSC in the plastomes of the Asparagales that contained a large number of pseudogenes, intron deletions and inversions (Supplementary Table S3). Most of these genes were related to transcription and translation, and are essential for land plants. The pseudogenes included accD, infA, rpl23, rpl32, rps2, rps16, rps19, and ycf1. The coding regions in the Nolinoideae plastomes of C. keiskei, L. spicata, Maianthemum bicolor, and Nolina atopocarpa were highly conserved, but differed to those of P. cyrtonema. The protein-coding regions of the Nolinoideae were extracted and evaluated in order to identify divergent hotspots in the coding regions (Supplementary Fig. S3). Most of the protein-coding genes were highly conserved; however, minor divergences were detected. The protein-coding genes accD, cemA, ccsA, matK, ndhF, rpl22, rpl32, and rps15 had slightly diverged in the Nolinoideae because of an indel in their LSC, IR and SSC regions. The infA gene had highly diverged due to the presence of a pseudogene in the Convallaria, Liriope and Nolina18 cp genomes.

The cp gene infA of C. keiskei and L. spicata was compared with those in other Asparagales and was found to be a pseudogene in both the C. keiskei and L. spicata cp genomes, whereas in the plastome genomes of M. bicolor and P. cyrtonema it was a functional infA gene. The functional infA gene sequence was highly variable in the Nolinoideae. The infA gene on the L. spicata cp genome was only 87 bp long, possibly due to a frameshift mutation caused by the insertion of 4 bp at a position between 59 and 62 bp. Two base pairs had been deleted at 183–184 bp in the infA gene of C. keiskei, resulting in it becoming a pseudogene with a total size of 186 bp. A similar pattern was observed in N. atopocarpa, in which 8 bp had been deleted in the infA gene that became a pseudogene with a total size of 72 bp. Phylogenetic analyses revealed that the loss of infA occurred independently in the Nolinoideae (Fig. 3). Previous studies have reported that the infA gene was independently lost from other monocotyledons, such as most of the Agavoideae, Allioideae, Aphyllanthoideae, Asphodeloideae, Brodiaeoideae, Lemnoideae, Lomandroideae, and other angiosperm lineages17–21. A comparison of the other plastome protein-coding genes of the Asparagales revealed that accD, rpl23, rpl32, rps2, rps16, rps19 and ycf1 are pseudogenes. Comparative analysis revealed that all of the pseudogene or gene loss occurred independently in the Asparagales, except for rps16 in the Lomandroideae (Fig. 3). However, the cp genomes of several species of the Lomandroideae should be investigated in order to elucidate the evolution of rps16. Although several cp genes were deleted during evolution, these deletions are not related to the taxonomy of the Asparagales. Taken together, these results suggest that most cp gene loss occurred independently across the Asparagales, as well as the angiosperms.

Figure 3.

Figure 3

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Molecular phylogenetic tree of 27 Asparagales taxa based on 68 protein-coding genes in the chloroplast genome. The tree was constructed by maximum likelihood analysis of the conserved regions using the RAxML program and the GTR + G + I nucleotide model. The stability of each tree node was tested by bootstrap analysis with 1,000 replicates. Bootstrap values are indicated on the branches, and the branch length reflects the estimated number of substitutions per 1,000 sites. Aloe vera and Xanthorrhoea preissii were set as the outgroups.

Synonymous and non-synonymous substitutions

In genome evolution studies, the ratio of non-synonymous (dN) to synonymous (dS) substitutions is an important indicator22. We calculated the dN/dS ratio in the Nolinoideae using C. keiskei as a reference genome. The majority of genes in the Nolinoideae genomes had ratios of less than 1.0, with the exceptions of matK, psbK, rbcL, cemA, and ycf2. ycf2 had the highest ratio (~2.28), followed by rbcL (1.7), cemA (1.6), matK (1.3) and psbK (1.15), indicating that these genes are not conserved in the cp genome (Fig. 4). cemA, rbcL and psbK are involved in photosynthesis, matK encodes maturase and the function of ycf2 is unknown. Although a missense mutation occurred in these genes, they are under positive selection in Nolinoideae cp genomes, possibly by adapting to changing ecological conditions. One-third of plastid genes, including self-replication- and photosynthesis-related genes, evolved under positive selection in the Poaceae23. In contrast, in the Nolinoideae, the substitution ratios were less than one for most of the photosynthetic-related genes, except for cemA, rbcL and psbK, which are transcription and translation genes that are more highly conserved than other genes in cp genomes because of strong functional constraints.

Figure 4.

Figure 4

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Ratio of non-synonymous (dN) to synonymous (dS) substitutions of 68 protein-coding genes in the Nolinoideae. Blue dots indicate the dN/dS ratio of Convallaria keiskei vs Maianthemum bicolor, orange dots indicate C. keiskei vs Liriope spicata, grey dots indicate C. keiskei vs Nolina atopocarpa, and yellow dots indicate C. keiskei vs Polygonatum cyrtonema.

Codon usage

Basal eudicots encode AUG as the initiation codon for most protein-coding genes, but the Convallaria and Liriope cp genomes encode an alternative starting codon (ACG) for rpl2. A similar type of codon was observed in all of the Asparagales cp genomes. An analysis of the codon usage patterns of 68 unique cp protein-coding genes in 27 Asparagales taxa revealed that 294,399 codons were present in the protein-coding genes. Figure 5 is a heatmap of codon usage in the Asparagales. A relative synonymous codon usage (RSCU) value of <1 (red colour) indicates weak codon bias, and a value of >1 (green colour) denotes strong codon bias. Figure 5 shows that the half of the codons (28) that ended with G/C (denoted in red) are not frequently used in the Asparagales, whereas the 31 codons that ended with A/T (denoted in green) and had high RSCU values are used by all species of Asparagales. Among these, the codons TTA and GCT had high RSCU values of 2.009791 and 1.897735, respectively. Similar results have been obtained in many other land plant and algal lineages24. The high RSCU values of the codons indicate amino acid functions or peptide structures that inhibit transcriptional errors in cp genomes.

Figure 5.

Figure 5

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Codon distributions of chloroplast protein-coding genes in the Asparagales. Green indicates a high relative synonymous codon usage (RSCU) value and red indicates a low RSCU value. Hierarchical clustering (average linkage method) was performed for the codon patterns (x-axis).

Phylogenetic analysis

To elucidate the phylogenetic relationships of the Asparagales, 68 cp protein-coding genes shared by 27 genomes were investigated. The phylogenetic tree was divided into three groups: the Xanthorrhoeaceae, Amaryllidaceae, and Asparagaceae (Fig. 3). The Xanthorrhoeaceae is basal to the rest of the Asparagales. Two major clades formed in the Asparagaceae, with the Lomandroideae, Asparagoideae and Nolinoideae in one clade and the Agavoideae, Aphyllanthoideae and Brodiaeoideae in the other. The Nolinoideae is a sister group to the Asparagoideae, with a 100% bootstrap (BS) value. In the Nolinoideae, Polygonatum is the basal group with a 100% BS value, and L. spicata is a sister species to M. bicolor and C. keiskei with a very weak 53% BS value, although the BS value of M. bicolor and C. keiskei was 76%. This weak BS value may have been caused by indels and nucleotide differences in the protein-coding genes of their respective cp genomes.

Divergence time estimation

The aim was to estimate divergence time for the Nolinoideae, but due to a lack of calibration points, we included other species of Asparagales. Divergence time was estimated using previous data of the Asparagales, which were similar to those obtained in the present study. In addition, the species used in both the maximum likelihood phylogenetic tree and the divergence analysis were the same. Among the Asparagales, the Xanthorrhoeaceae basal group (Aloe vera and Xanthorrhoea preissii) diverged 54.35 million years ago (mya) (95% highest posterior density (HPD): 65.48–43.54 mya), followed by the Amaryllidaceae at 51.61 mya (95% HPD: 61.78–41.51 mya) (Fig. 6). In the Asparagaceae, the Asparagoideae (A. officinalis) is the sister group to the Nolinoideae and diverged at 44.09 mya (95% HPD: 56.33–30.09 mya). Chronogram results from a BEAST analysis revealed that all of the speciation events within Nolinoideae occurred from 56.33 to 2.97 mya. Polygonatum diverged from the ancestor of all other members of the Nolinoideae at 19.73 mya (95% HPD: 33.35–10.04 mya), whereas Liriope diverged at 8.67 mya (95% HPD: 15.61–3.85 mya). Among the Nolinoideae, C. keiskei and M. bicolor diverged over a relatively short amount of time at 7.68 mya (95% HPD: 14.55–2.97 mya). Interestingly, mtDNA transfer only occurred in the cp genome of C. keiskei, and the pseudogenization of infA occurred in all species of the Nolinoideae except for M. bicolor. Although M. bicolor is a sister species to C. keiskei, the M. bicolor genome was highly conserved during evolution and could have recently diverged. In order to confirm this, more Nolinoideae cp genomes should be investigated.

Figure 6.

Figure 6

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Molecular clock based on 68 protein-coding genes of 27 Asparagales chloroplast genomes using BEAST. Mean age estimates (in millions of years) are shown along the branches. Grey bars represent the 95% posterior density credibility intervals for node ages.

Analysis of mtDNA transfer into the C. keiskei cp genome

Whole-genome cp sequences are ideal for comparing mtDNA transfer into the cp genome in C. keiskei with that in other species of the Nolinoideae through phylogenetic analysis, but there is a lack of whole-genome plastome sequence data that cover all Nolinoideae species. Therefore, in the present study, four cp marker genes (matK, rbcL, atpB, and ndhF) were used to investigate the relationship between C. keiskei and other species of the Nolinoideae. The phylogenetic results showed that Eriospermum flagelliforme is the basal group in the Nolinoideae, and there were three major clades with weak BS values: N. atopocarpa formed one clade, C. keiskei and L. spicata formed the second, and M. bicolor and Polygonatum formed the third (Fig. 7). However, C. keiskei and L. spicata formed sister clades. In order to place the mtDNA transfer event in an evolutionary context, species in the Convallaria clade (Speirantha gardenii, Aspidistra elatior, Reineckea carnea, Camphylandra aurantiaca, and Rohdea japonica) were used to identify whether they shared the mt segment of DNA that is in the C. keiskei plastome genome. Gene-specific primers were designed to determine whether the mt spacer region in Convallaria was similar in size to that in other closely related species of Nolinoideae (Fig. 8a). Polymerase chain reaction (PCR) results confirmed the presence of mtDNA in only the C. keiskei cp genome, not in other species of Nolinoideae (Fig. 8b). Three further primers were used to confirm the presence of mtDNA in the C. keiskei cp genome (Fig. 8a), and a PCR confirmed that mtDNA was present in the intergenic regions between ycf2 and trnL (Fig. 8b). In addition, all Illumina sequencing reads were mapped to the C. keiskei plastome assembly to understand whether the presence of the mt-like insertion in the IR region belongs to the cp genome of C. keiskei. The analysis showed that the depth coverage of C. keiskeii plastome is ~428× and the coverage is uniformly distributed across the cp genome regions (Fig. 8c). Also, the depth coverage of mt-like insertion region is very similar to cp genome regions of C. keiskei. Correspondingly, all Illumina sequencing reads were mapped with mitochondrial genes of Amborella (as a reference) to identify the depth coverage of mitochondrial genes of C. keiskei. The depth coverage of C. keiskei mitochondrial genes is ~15× (data not shown). Therefore, the analysis confirmed that the presence of the mt-like insertion in the IR region is associated with the cp genome of C. keiskei.

Figure 7.

Figure 7

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Molecular phylogenetic tree analysis of four chloroplast genes (atpB, matK, ndhF and rbcL) in the Nolinoideae subfamily. The tree was constructed by maximum likelihood analysis of the conserved regions using the RAxML program and the GTR + G + I nucleotide model. The stability of each tree node was tested by bootstrap analysis with 1,000 replicates. Bootstrap values are indicated on the branches, and the branch length reflects the estimated number of substitutions per 1,000 sites. Asparagus officinalis was set as the outgroup.

Figure 8.

Figure 8

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Analysis of mitochondrial DNA transfer in the chloroplast (cp) genome. (a) Position of four sets of gene-specific primers in the cp genome of Convallaria keiskei. (b) Polymerase chain reaction confirmation of the presence or absence of mitochondrial DNA in the Nolinoideae subfamily cp genome using agarose gel electrophoresis. Lane M: SolGent™ 1 kb Plus DNA Ladder; Lanes 1–4: C. keiskei cp genome (Lane 1: ycf2–ndhB, Lane 2: rpl2 mitochondrial DNA region, Lane 3: mitochondrial DNA region–rrn16, and Lane 4: rpl2–rrn16); Lane 5: Reineckea carnea; Lane 6: Rohdea japonica; Lane 7: Tupistra aurantiaca; Lane 8: Speirantha gardenii; and Lane 9: Aspidistra spp. (c) Sequencing-depth coverage of the mitochondrial DNA insert region (94,989–98,301 bp) in the C. keiskei cp genome.

A total of 3,313 mt-like nucleotide sequences were identified in the C. keiskei cp genome using BLASTN. These sequences were integrated upstream of tRNALeu (trnL) and downstream of ycf2 (94,989–98,301 bp in the IRB region) in the plastid genome (Supplementary Fig. S4). The inserted sequences included intergenic regions of the mitogenome, fragments of mt genes, and sequences of unknown providence. Illumina sequencing reads were mapped to the C. keiskei plastome assembly and showed that it was uniformly distributed across the region and confirmed the presence of a mt-like insertion in the IR region (Fig. 8c). The mt-like sequences were BLASTed against all other genes in the US National Centre for Biotechnology Information (NCBI) database, which revealed that 994 nucleotide sequences had 93.2% similarity with the mt genome of the monocotyledon Cocos nucifera. Of the 994 bp, 386 bp were identical to the partial mt rpl10 gene, which was present as a pseudogene (Supplementary Fig. S5). The remaining 608 bp were in the intergenic regions of trnL and rpl10. Previous studies have found that IGT in the genome, even if the fragment comprises a gene or genes in higher land plants, usually does not encode any functional property in the recipient genome4,5,7, and intracellularly transferred DNA is generally only transiently maintained in the recipient genome over evolutionary time4,5,7. Despite this, the presence of a ~2 kb DNA insert sequence in the C. keiskei cp genome shows that there is no homology with the mt genomes of any other land plants or sequences in GenBank. The lack of mt and nuclear genomic information for C. keiskei limits our ability to investigate the unknown ~2 kb DNA insert in the C. keiskei cp genome, but it could have been caused by a unique sequence in the C. keiskei mt or nuclear genomes.

Cp genome segments ranging from 17 to 130 kb in length have been identified in the mt genomes of many monocotyledons25–31 and seed plants10,26,32–39, and plastid DNA has been found in the nuclear genomes of Arabidopsis, soybean and other species40,41. In addition, nuclear DNA has migrated into the mt genomes of Cucumis melo9, Arabidopsis42, maize43, sugar beet44, rice30, and wheat45, and migration in the opposite direction (mtDNA to the nuclear genome) has also been reported in several angiosperms46–48. However, DNA transfer from the mt to the cp has only been identified in four families of land plants. In the dicotyledons Daucus and Cuminum, 1.5 kb of mtDNA has been found in the rps12–trnV intergenic spacer of IR regions11–13, Asclepias syriaca (milkweed) has 2.4 kb of mt-like DNA in the rps2–rpoC2 intergenic spacer of the LSC region14, and 6.7 kb of mtDNA has been inserted in ycf2–trnL in the plastid IR regions of cashew (A. occidentale) plastomes15. Regarding monocotyledons, 2.7 kb of mtDNA has been inserted into the trnI-CAU–trnL-CAA intergenic spacer in IR regions of the herbaceous bamboo Pariana16. In C. keiskei, 3.3 kb of mtDNA is in the intergenic spacer of ycf2–trnL in plastid IR regions. The location of the mtDNA insertion in the IR region (intergenic region between ycf2 and trnL) of the C. keiskei plastome is similar to that in the A. occidentale plastid genome15. However, the sizes of the mtDNA insertions and insertion sequences vary among genomes. When comparing the insertion of mtDNA with the other four plastomes, common IGT insertion events occur in the non-coding spacer regions of the plastomes. This integration may be random or is facilitated by homologous recombination; however, any interruption in plastid coding sequences that decreases overall fitness would probably be purged15. Among the mtDNA-integrated plastomes of land plants, a high GC content was observed in C. keiskei (45.7%), followed by Pariana (44.7%), Daucus (44.0%), A. occidentale (43.5%) and milkweed (40.3%). In addition, no deletions of plastid sequences were observed in the mtDNA-inserted region in the C. keiskei plastome, and the same was true of the milkweed and cashew plastid genomes. However, mtDNA insertion was accompanied by the deletion from their plastid genomes of 339 bp in the carrot and 1,379 bp in bamboo. All of these studies reported that mtDNA transfer occurred in common ancestors, such as Apiaceae for Daucus and Cuminum, Apocynaceae for milkweed, and Anacardium and bamboo for Pariana and Eremitis. However, we found that mtDNA transfer only occurred in the C. keiskei cp genome, and not in the common ancestor plants of the Nolinoideae. Further studies are required to ascertain why mtDNA insertion is restricted to the C. keiskei cp genome of the Nolinoideae.

How mtDNA was integrated into the C. keiskei cp genome is unknown. Because plastids have a double membrane, they are generally unable to take up DNA4,5,7,8,14; however, stress, transformation, and double-membrane breach can result in the uptake of foreign DNA. Although gene transfer could occur by IGT, there is no evidence that it plays a role in the IGT mechanism. Further studies are needed to understand intra- and inter-genomic DNA transfer and recombination in the cp genome.

Conclusion

he C. keiskei plastome is longer than that of most monocotyledon flowering plants due to an insertion of ~3.3 kb of mtDNA in the IR regions. However, some variations were identified. To the best of our knowledge, this is the first report that C. keiskei has the largest cp genome in the Asparagaceae, and the second report of mtDNA transfer into monocotyledon plastomes. Analysis of the large amount of angiosperm cp genome sequence data in the NCBI database strongly suggests that this is a rare event in monocotyledons. Taken together, these findings indicate that the C. keiskei cp genome is unique amongst monocotyledons, and further investigation of the mt genome is required to understand the direction and mechanism of mtDNA uptake by the plastid genome in C. keiskei.

Methods

Genomic DNA extraction and sequencing

Fresh leaf material from C. keiskei and L. spicata was collected from the Choijung and Palgong mountains, South Korea, respectively. Total genomic DNA was extracted using a modified cetyl trimethylammonium bromide method49. Whole-genome sequencing was performed using an Illumina HiSeq 2500 (Phyzen Ltd., South Korea) and a paired-end (PE) library of 2 × 150 bp and an insert size of ~550 bp. A total of 33,272,066 and 26,965,288 raw reads of C. keiskei and L. spicata, respectively, were obtained, and PE Illumina reads were assembled de novo using Velvet v1.2.1050 with multiple k-mers. The initial plastid contigs of both genomes were assembled using Geneious v7.1.8 (Biomatters, New Zealand). The sequencing data and gene annotations of both genomes were submitted to GenBank and assigned accession numbers of MH60946 for C. keiskei and MH60945 for L. spicata.

Cp genome annotation and analysis

The initial annotation of the cp genomes was conducted using the online DOGMA tool51. From this initial annotation, putative starts and stops, and intron positions were identified based on comparisons with homologous genes in N. atopocarpa, P. cyrtonema, and Nicotiana tabacum. The tRNAs identified were confirmed using tRNAscan-SE52. A circle map of cp genomes was drawn using the OGDRAW program53.

Comparative genome analysis

The complete cp genome sequences of both C. keiskei and L. spicata were compared with 14 other cp genomes in the Asparagales using the mVISTA program in Shuffle-LAGAN mode54. The C. keiskei cp genome was set as a reference. Boundary regions between the LSC, IR and SSC and their lengths were compared and analysed using cp genomes of the Asparagaceae.

Analysis of dS and dN substitution rates

The C. keiskei cp genome sequence was compared with those of the Nolinoideae species L. spicata, M. bicolor, N. atopocarpa and P. cyrtonema. dS and dN substitution rates were analysed by the same individual, and functional protein-coding gene exons were separately extracted and aligned using Geneious v10.2.4. The aligned sequences were translated into protein sequences and analysed by DnaSP55.

Codon usage

Codon usage was determined for all protein-coding genes of Asparagales cp genomes. Codon-usage distributions were visualised in the form of heatmaps of 27 species of Asparagales, and histograms were generated using the Heatmapper program with RSCU values56. RSCU values were calculated by counting the number of times a particular codon was observed, relative to all codons for a given amino acid that exhibited similar probabilities57. A RSCU value of <1.00 indicated a codon that was used less frequently than expected, and a value of >1.00 indicated a codon that was used more frequently than expected.

Phylogenetic tree

The jModelTest 2 v0.1.10 program was used to analyse the general GTR + G + I model for protein-coding sequences using optimized parameters58. Phylogenetic analyses of four cp genes in the Nolinoideae subfamily (atpB, matK, ndhF, and rbcL) and 68 protein-coding genes in 27 Asparagales cp genomes were separately performed using the maximum likelihood method in RAxML v7.2.6 with 1,000 BS replicates59.

PCR amplification of mtDNA insertions in Nolinoideae species

In order to detect mtDNA insertions in the cp genomes of the Nolinoideae, DNA samples of Aspidistra spp., Reineckea carnea (Andrews) Kunth, Rohdea japonica (Thunb.) Roth, Speirantha gardenii (Hook.) Baill. and Tupistra aurantiaca (Baker) Wall ex Hook.f. were obtained from the DNA Bank of the Royal Botanic Gardens, Kew (http://data.kew.org/dnabank/DnaBankForm.html) and used as templates, and gene-specific primers that were designed based on the plastid genes between ycf2 and trnL (1F and 1R) were used. Further, to confirm the presence of mtDNA in the C. keiskei cp genome, three different primer sets were designed (Fig. 8a; Supplementary Table S4). The first primer was between rpl2 and the mtDNA region (2F and 2R), the second was between the mtDNA region and rrn16 (3F and 3R), and the third was between rpl2 and rrn16 (4F and 4R). All of these primers were designed using Primer3 in Geneious v7.1.8. Long PCR products were amplified using gene-specific primers, and the PCR mixture (25 μL) contained 10 pM of primer, 250 ng of genomic DNA, 1X PrimeSTAR GXL buffer with 1 mM MgCl2, 0.2 mM of each deoxynucleotide triphosphate (dNTP), and 0.625 U/μL of PrimeSTAR GXL DNA Polymerase (Takara Bio Inc., CA, USA). A two-step PCR amplification was performed in an Arktik thermal cycler (Thermo Fisher Scientific, MA, USA) programmed with an initial denaturation at 98 °C for 2 min, 30 cycles at 98 °C for 10 s, 68 °C for 10 min and 68 °C for 10 min. The 2-μL PCR product was separated using 1.5% agarose gel stained with ethidium bromide staining solution in 1X tris acetate ethylenediaminetetraacetic acid. The image of the gel was digitized using the UVITEC Cambridge system (Cleaver Scientific Ltd., Warwickshire, UK).

Molecular evolution tree

A total of 68 protein-coding genes from 27 Asparagales cp genomes were used for the molecular divergence analyses. A molecular clock tree was constructed using BEAST v2.1 (Centre for Computational Evolution, University of Auckland, New Zealand)60. A relaxed clock log-normal model was implemented using Markov Chain Monte Carlo chains that were run for 300 million generations with a 10% burn-in and were sampled every 1,000 generations. A GTR nucleotide substitution model was used with a gamma distribution and four rate categories. A Yule tree prior was used to estimate divergence times and creditability intervals. The sample size was evaluated using Tracer v1.6 analysis software (Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, UK)61, and tree data were summarised using TreeAnnotator v2.1.2 (Centre for Computational Evolution, University of Auckland, New Zealand)60. Multiple calibration points were set for the divergence of the Amaryllidaceae at 51.2 ± 6.0 mya (42–61.8 mya), for the divergence of the Asparagaceae at 56.4 ± 5.3 mya (48.1–65.5 mya), and for the divergence of the Xanthorrhoeaceae at 55.6 ± 5.5 mya (48–66.0 mya)62, and implemented with a log-normal distribution.

Supplementary information

Supplementary files (1,003.8KB, pdf)

Acknowledgements

This work was supported by the 2017 Yeungnam University Grant (217A380095), South Korea.

Author Contributions

S.J.P., G.R., and S.P. conceived and designed the project. S.J.P. supervised the project. G.R., S.P., and E.M.L. performed the experiments. G.R. and S.P. analysed the data. G.R. prepared a draft of the manuscript and figures. All authors edited and approved the final version of the manuscript for submission.

Competing Interests

The authors declare no competing interests.

Footnotes

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Supplementary information accompanies this paper at 10.1038/s41598-019-41377-w.

References

  • 1.Archibald JM. Origin of eukaryotic cells: 40 years on. Symbiosis. 2011;54:69–86. doi: 10.1007/s13199-011-0129-z. [DOI] [Google Scholar]
  • 2.Martin W, et al. Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus. Proceedings of the National Academy of Sciences of the United States of America. 2002;99:12246–12251. doi: 10.1073/pnas.182432999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Timmis JN, Ayliffe MA, Huang CY, Martin W. Endosymbiotic gene transfer: organelle genomes forge eukaryotic chromosomes. Nature reviews. Genetics. 2004;5:123–135. doi: 10.1038/nrg1271. [DOI] [PubMed] [Google Scholar]
  • 4.Bock R. The give-and-take of DNA: horizontal gene transfer in plants. Trends in plant science. 2010;15:11–22. doi: 10.1016/j.tplants.2009.10.001. [DOI] [PubMed] [Google Scholar]
  • 5.Keeling PJ, Palmer JD. Horizontal gene transfer in eukaryotic evolution. Nature reviews. Genetics. 2008;9:605–618. doi: 10.1038/nrg2386. [DOI] [PubMed] [Google Scholar]
  • 6.Renner, S. S. & Bellot, S. In Genomics of Chloroplasts and Mitochondria (eds Ralph Bock & Volker Knoop) 223–235 (Springer Netherlands, 2012).
  • 7.Richardson AO, Palmer JD. Horizontal gene transfer in plants. Journal of experimental botany. 2007;58:1–9. doi: 10.1093/jxb/erl148. [DOI] [PubMed] [Google Scholar]
  • 8.Smith DR. Extending the limited transfer window hypothesis to inter-organelle DNA migration. Genome biology and evolution. 2011;3:743–748. doi: 10.1093/gbe/evr068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Alverson AJ, Rice DW, Dickinson S, Barry K, Palmer JD. Origins and recombination of the bacterial-sized multichromosomal mitochondrial genome of cucumber. The Plant cell. 2011;23:2499–2513. doi: 10.1105/tpc.111.087189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Alverson AJ, et al. Insights into the evolution of mitochondrial genome size from complete sequences of Citrullus lanatus and Cucurbita pepo (Cucurbitaceae) Molecular biology and evolution. 2010;27:1436–1448. doi: 10.1093/molbev/msq029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Iorizzo M, et al. Against the traffic: The first evidence for mitochondrial DNA transfer into the plastid genome. Mobile genetic elements. 2012;2:261–266. doi: 10.4161/mge.23088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Goremykin VV, Salamini F, Velasco R, Viola R, Mitochondrial DNA. of Vitis vinifera and the issue of rampant horizontal gene transfer. Molecular biology and evolution. 2009;26:99–110. doi: 10.1093/molbev/msn226. [DOI] [PubMed] [Google Scholar]
  • 13.Iorizzo M, et al. De novo assembly of the carrot mitochondrial genome using next generation sequencing of whole genomic DNA provides first evidence of DNA transfer into an angiosperm plastid genome. BMC plant biology. 2012;12:61. doi: 10.1186/1471-2229-12-61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Straub SC, Cronn RC, Edwards C, Fishbein M, Liston A. Horizontal transfer of DNA from the mitochondrial to the plastid genome and its subsequent evolution in milkweeds (apocynaceae) Genome biology and evolution. 2013;5:1872–1885. doi: 10.1093/gbe/evt140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Rabah, S. O. et al. Plastome Sequencing of Ten Nonmodel Crop Species Uncovers a Large Insertion of Mitochondrial DNA in Cashew. The plant genome10, 10.3835/plantgenome2017.03.0020 (2017). [DOI] [PubMed]
  • 16.Ma PF, Zhang YX, Guo ZH, Li DZ. Evidence for horizontal transfer of mitochondrial DNA to the plastid genome in a bamboo genus. Scientific reports. 2015;5:11608. doi: 10.1038/srep11608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Kim HT, Kim JS, Kim JH. The complete plastid genome sequence of Eustrephus latifolius (Asparagaceae: Lomandroideae) Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis. 2016;27:1549–1551. doi: 10.3109/19401736.2014.953132. [DOI] [PubMed] [Google Scholar]
  • 18.McKain MR, et al. Timing of rapid diversification and convergent origins of active pollination within Agavoideae (Asparagaceae) American journal of botany. 2016;103:1717–1729. doi: 10.3732/ajb.1600198. [DOI] [PubMed] [Google Scholar]
  • 19.Mardanov AV, et al. Complete sequence of the duckweed (Lemna minor) chloroplast genome: structural organization and phylogenetic relationships to other angiosperms. Journal of molecular evolution. 2008;66:555–564. doi: 10.1007/s00239-008-9091-7. [DOI] [PubMed] [Google Scholar]
  • 20.Sheng W, Chai X, Rao Y, Tu X, Du S. Complete chloroplast genome sequence of Asparagus (asparagus officinalis l.) and its phylogenetic position within asparagales. Journal of Plant Breeding and Genetics. 2017;5:8. [Google Scholar]
  • 21.Wang W, Messing J. High-throughput sequencing of three Lemnoideae (duckweeds) chloroplast genomes from total DNA. PloS one. 2011;6:e24670. doi: 10.1371/journal.pone.0024670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Kimura, M. The Neutral Theory of Molecular Evolution. (Cambridge University Press, 1983).
  • 23.Piot A, Hackel J, Christin P-A, Besnard G. One-third of the plastid genes evolved under positive selection in PACMAD grasses. Planta. 2018;247:255–266. doi: 10.1007/s00425-017-2781-x. [DOI] [PubMed] [Google Scholar]
  • 24.Morton BR. Selection on the codon bias of chloroplast and cyanelle genes in different plant and algal lineages. Journal of molecular evolution. 1998;46:449–459. doi: 10.1007/PL00006325. [DOI] [PubMed] [Google Scholar]
  • 25.Cummings MP, Nugent JM, Olmstead RG, Palmer JD. Phylogenetic analysis reveals five independent transfers of the chloroplast gene rbcL to the mitochondrial genome in angiosperms. Current genetics. 2003;43:131–138. doi: 10.1007/s00294-003-0378-3. [DOI] [PubMed] [Google Scholar]
  • 26.Fang Y, et al. A complete sequence and transcriptomic analyses of date palm (Phoenix dactylifera L.) mitochondrial genome. PloS one. 2012;7:e37164. doi: 10.1371/journal.pone.0037164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Leon P, Walbot V, Bedinger P. Molecular analysis of the linear 2.3 kb plasmid of maize mitochondria: apparent capture of tRNA genes. Nucleic acids research. 1989;17:4089–4099. doi: 10.1093/nar/17.11.4089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Lin CS, et al. The location and translocation of ndh genes of chloroplast origin in the Orchidaceae family. Scientific reports. 2015;5:9040. doi: 10.1038/srep09040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.Nakazono M, Hirai A. Identification of the entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice. Molecular & general genetics: MGG. 1993;236:341–346. doi: 10.1007/BF00277131. [DOI] [PubMed] [Google Scholar]
  • 30.Notsu Y, et al. The complete sequence of the rice (Oryza sativa L.) mitochondrial genome: frequent DNA sequence acquisition and loss during the evolution of flowering plants. Molecular genetics and genomics: MGG. 2002;268:434–445. doi: 10.1007/s00438-002-0767-1. [DOI] [PubMed] [Google Scholar]
  • 31.Zheng D, Nielsen BL, Daniell H. A 7.5-kbp region of the maize (T cytoplasm) mitochondrial genome contains a chloroplast-like trnI (CAT) pseudo gene and many short segments homologous to chloroplast and other known genes. Current genetics. 1997;32:125–131. doi: 10.1007/s002940050256. [DOI] [PubMed] [Google Scholar]
  • 32.Alverson AJ, Zhuo S, Rice DW, Sloan DB, Palmer JD. The mitochondrial genome of the legume Vigna radiata and the analysis of recombination across short mitochondrial repeats. PloS one. 2011;6:e16404. doi: 10.1371/journal.pone.0016404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Hao W, Palmer JD. Fine-scale mergers of chloroplast and mitochondrial genes create functional, transcompartmentally chimeric mitochondrial genes. Proceedings of the National Academy of Sciences of the United States of America. 2009;106:16728–16733. doi: 10.1073/pnas.0908766106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Marienfeld J, Unseld M, Brennicke A. The mitochondrial genome of Arabidopsis is composed of both native and immigrant information. Trends in plant science. 1999;4:495–502. doi: 10.1016/S1360-1385(99)01502-2. [DOI] [PubMed] [Google Scholar]
  • 35.Nakazono M, Nishiwaki S, Tsutsumi N, Hirai A. A chloroplast-derived sequence is utilized as a source of promoter sequences for the gene for subunit 9 of NADH dehydrogenase (nad9) in rice mitochondria. Molecular & general genetics: MGG. 1996;252:371–378. doi: 10.1007/BF02173001. [DOI] [PubMed] [Google Scholar]
  • 36.Rice DW, et al. Horizontal transfer of entire genomes via mitochondrial fusion in the angiosperm Amborella. Science (New York, N.Y.) 2013;342:1468–1473. doi: 10.1126/science.1246275. [DOI] [PubMed] [Google Scholar]
  • 37.Rodriguez-Moreno L, et al. Determination of the melon chloroplast and mitochondrial genome sequences reveals that the largest reported mitochondrial genome in plants contains a significant amount of DNA having a nuclear origin. BMC genomics. 2011;12:424. doi: 10.1186/1471-2164-12-424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Tomohiko K, Tetsuo M. Organization and variation of angiosperm mitochondrial genome. Physiologia Plantarum. 2007;129:6–13. doi: 10.1111/j.1399-3054.2006.00768.x. [DOI] [Google Scholar]
  • 39.Veronico P, Gallerani R, Ceci LR. Compilation and classification of higher plant mitochondrial tRNA genes. Nucleic acids research. 1996;24:2199–2203. doi: 10.1093/nar/24.12.2199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Gantt, J. S., Baldauf, S. L., Calie, P. J., Weeden, N. F. & Palmer, J. D. Transfer of rpl22 to the nucleus greatly preceded its loss from the chloroplast and involved the gain of an intron. The EMBO journal10, 3073-3078 (1991). [DOI] [PMC free article] [PubMed]
  • 41.Millen RS, et al. Many parallel losses of infA from chloroplast DNA during angiosperm evolution with multiple independent transfers to the nucleus. The Plant cell. 2001;13:645–658. doi: 10.1105/tpc.13.3.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 42.Stupar RM, et al. Complex mtDNA constitutes an approximate 620-kb insertion on Arabidopsis thaliana chromosome 2: implication of potential sequencing errors caused by large-unit repeats. Proceedings of the National Academy of Sciences of the United States of America. 2001;98:5099–5103. doi: 10.1073/pnas.091110398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Clifton SW, et al. Sequence and comparative analysis of the maize NB mitochondrial genome. Plant physiology. 2004;136:3486–3503. doi: 10.1104/pp.104.044602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 44.Kubo T, et al. The complete nucleotide sequence of the mitochondrial genome of sugar beet (Beta vulgaris L.) reveals a novel gene for tRNA(Cys)(GCA) Nucleic acids research. 2000;28:2571–2576. doi: 10.1093/nar/28.13.2571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Ogihara Y, et al. Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome. Nucleic acids research. 2005;33:6235–6250. doi: 10.1093/nar/gki925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 46.Adams KL, Qiu YL, Stoutemyer M, Palmer JD. Punctuated evolution of mitochondrial gene content: high and variable rates of mitochondrial gene loss and transfer to the nucleus during angiosperm evolution. Proceedings of the National Academy of Sciences of the United States of America. 2002;99:9905–9912. doi: 10.1073/pnas.042694899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 47.Liu SL, Zhuang Y, Zhang P, Adams KL. Comparative analysis of structural diversity and sequence evolution in plant mitochondrial genes transferred to the nucleus. Molecular biology and evolution. 2009;26:875–891. doi: 10.1093/molbev/msp011. [DOI] [PubMed] [Google Scholar]
  • 48.Park S, et al. Dynamic evolution of Geranium mitochondrial genomes through multiple horizontal and intracellular gene transfers. The New phytologist. 2015;208:570–583. doi: 10.1111/nph.13467. [DOI] [PubMed] [Google Scholar]
  • 49.Doyle JJ. Isolation of plant DNA from fresh tissue. Focus. 1990;12:13–15. [Google Scholar]
  • 50.Zerbino DR, Birney E. Velvet: Algorithms for de novo short read assembly using de Bruijn graphs. Genome research. 2008;18:821–829. doi: 10.1101/gr.074492.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 51.Wyman SK, Jansen RK, Boore JL. Automatic annotation of organellar genomes with DOGMA. Bioinformatics (Oxford, England) 2004;20:3252–3255. doi: 10.1093/bioinformatics/bth352. [DOI] [PubMed] [Google Scholar]
  • 52.Schattner P, Brooks AN, Lowe TM. The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs. Nucleic acids research. 2005;33:W686–689. doi: 10.1093/nar/gki366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 53.Lohse M, Drechsel O, Bock R. OrganellarGenomeDRAW (OGDRAW): a tool for the easy generation of high-quality custom graphical maps of plastid and mitochondrial genomes. Current genetics. 2007;52:267–274. doi: 10.1007/s00294-007-0161-y. [DOI] [PubMed] [Google Scholar]
  • 54.Frazer KA, Pachter L, Poliakov A, Rubin EM, Dubchak I. VISTA: computational tools for comparative genomics. Nucleic acids research. 2004;32:W273–279. doi: 10.1093/nar/gkh458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 55.Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics (Oxford, England) 2009;25:1451–1452. doi: 10.1093/bioinformatics/btp187. [DOI] [PubMed] [Google Scholar]
  • 56.Babicki S, et al. Heatmapper: web-enabled heat mapping for all. Nucleic acids research. 2016;44:W147–153. doi: 10.1093/nar/gkw419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 57.Wang Y, et al. Complete Chloroplast Genome Sequence of Aquilaria sinensis (Lour.) Gilg and Evolution Analysis within the Malvales Order. Frontiers in plant science. 2016;7:280. doi: 10.3389/fpls.2016.00280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 58.Posada D. jModelTest: phylogenetic model averaging. Molecular biology and evolution. 2008;25:1253–1256. doi: 10.1093/molbev/msn083. [DOI] [PubMed] [Google Scholar]
  • 59.Stamatakis A, Hoover P, Rougemont J. A rapid bootstrap algorithm for the RAxML Web servers. Systematic biology. 2008;57:758–771. doi: 10.1080/10635150802429642. [DOI] [PubMed] [Google Scholar]
  • 60.Bouckaert R, et al. BEAST 2: a software platform for Bayesian evolutionary analysis. PLoS computational biology. 2014;10:e1003537. doi: 10.1371/journal.pcbi.1003537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 61.Rambaut, A., Drummond, A. J., Xie, D., Baele, G. & Suchard, M. A. Posterior Summarization in Bayesian Phylogenetics Using Tracer 1.7. Systematic biology, syy032–syy032, 10.1093/sysbio/syy032 (2018). [DOI] [PMC free article] [PubMed]
  • 62.Chen S, Kim DK, Chase MW, Kim JH. Networks in a large-scale phylogenetic analysis: reconstructing evolutionary history of Asparagales (Lilianae) based on four plastid genes. PloS One. 2013;8:e59472. doi: 10.1371/journal.pone.0059472. [DOI] [PMC free article] [PubMed] [Google Scholar]

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