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Microarray strategy to study OGT substrate preferences. A large number of immobilized peptides or proteins on the chip are incubated with OGT and UDP-GlcNAc (or a sugar donor analogue). Several methods can be used for detection of O-GlcNAcylation, including click chemistry of azido sugar with alkyne-linked biotin and detection by fluorescent streptavidin, anti-O-GlcNAc antibodies, and scintillation counting.
