• Prepare soluble substance for use. |
• Mix soluble substance with test sample. |
• Prepare a dilution control containing equal volumes of test sample and an inert substance, such as albumin or saline. |
• Incubate at optimal temperature and time. |
• Test the sample plus the substance and the sample plus the inert substance (dilution control) against RBCs that previously gave a positive reaction. |
RBCs = red blood cells.