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. Author manuscript; available in PMC: 2019 Mar 27.
Published in final edited form as: Immunohematology. 2019 Jan;35(1):19–22.
• Prepare soluble substance for use.
• Mix soluble substance with test sample.
• Prepare a dilution control containing equal volumes of test sample and an inert substance, such as albumin or saline.
• Incubate at optimal temperature and time.
• Test the sample plus the substance and the sample plus the inert substance (dilution control) against RBCs that previously gave a positive reaction.

RBCs = red blood cells.