Figure 1.

High expression levels of SIRT2, cMYC, and p-PDHA1 were found in CCA and are associated with poor prognosis.

(A) The expression levels of SIRT2, cMYC, and p-PDHA1 in a normal bile duct epithelial cell line (HIBEpic) and two human CCA cell lines (HuCCT1 and RBE) were determined by western blotting. (B) CCA cells were overexpressed with either SIRT2 plasmids or control vectors; the cells were then collected and lysed and the concentrations of metabolites were measured by MS. (C & D) The SIRT2, cMYC, and p-PDHA1 protein levels in the tumors and adjacent tissues from 60 CCA patients were detected by IHC and quantified. The magnification is ×200. Scale bars, 100 μm. (E) The 3-year survival was evaluated in CCA patients with SIRT2, cMYC, and p-PDHA1 protein expressions, respectively. Data represent mean ± SEM, n ≥ 3. **P < .01.