Fig. 2.

Genetic deletion of osteoblastic Plekho1 leads to amelioration of joint inflammation in CIA mice. (a) Time course changes in arthritis score from Plekho1_osx^−/− mice and WT mice (including Plekho1^fl/fl mice, Osx^+/−;Plekho1^fl/− mice, and Osx^+/− mice) after induction with type II chicken collagen. (b) Representative histological images in the ankle joint of hind paws from Plekho1_osx^−/−-CIA mice and WT-CIA mice on day 49 after primary immunization. Scale bars, 50 μm. (c) Histological inflammation score in the ankle joint of hind paws from Plekho1_osx^−/−-CIA mice and WT-CIA mice on day 49 after primary immunization. (d) Time course changes of IL-1β and IL-6 levels in the ankle joint of hind paws from Plekho1_osx^−/−-CIA mice and WT-CIA mice by ELISA examination. (e) A schematic diagram illustrating the design of the co-culture experiments. The CD4 + T lymphocytes or fibroblast-like synoviocytes (FLS) derived from WT mice were co-cultured with the osteoblasts derived from Plekho1_osx^−/− mice or WT mice, with or without the stimulation of TNF-α. (f) IL-1β and IL-6 mRNA levels in CD4 + T lymphocytes after co-culture with osteoblasts from WT mice and Plekho1_osx^−/− mice. (g) IL-1β and IL-6 mRNA levels in FLS after co-culture with osteoblasts from WT mice and Plekho1_osx^−/− mice. (h) Migration of neutrophils after co-culture with osteoblasts from WT mice and Plekho1_osx^−/− mice. All data are the mean ± s.d. n = 10 per group. *P < 0.05, **P < 0.01. For a, Two-way ANOVA with subsequent Bonferroni posttests was performed. For c & d, a Student's t-test was performed. For f-h, a one-way ANOVA with subsequent Tukey's multiple comparisons test was performed.