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. 2019 Mar 5;41:670–682. doi: 10.1016/j.ebiom.2019.02.057

Fig. 3.

Fig. 3

miR-4739 mediates MMT and collagen-I synthesis in human PMCs. (a-c) Human PMCs were transducted with recombinant lentivirus vector containing miR-4739 or scrambled negative control (vector control) plasmids. (a) After 72 h, relative mRNA levels of miR-4739 were determined by RT-qPCR. n = 6, #P < 0.05 versus vector control (Student's t-test). (b) Changes of protein levels of collagen-I, α-SMA, vimentin, E-cadherin and cytokeratin-8 were measured by Western blotting after 96 h. n = 3, #P < 0.05 versus vector control (Student's t-test). (c) Changes in cell proliferation determined by CCK-8 kit after 72 h. n = 12, #P < 0.05 versus vector control (Student's t-test). (d-e) After 72 h, Representative image of cells migration and line graph depicting changes in recovery area in percentage of the initial scratched area. n = 5, #P < 0.05 versus vector control (Student's t-test).