Fig. 3.

FGF21 transiently increases food intake and decreases lean mass in NZO mice. Mice were treated as described in Fig. 2. (a) Daily food intake and (b, c) averaged daily food intake during (b) the first 3 days of FGF21 treatment (day −3 until 0) and (c) days 1–7. Changes in (d) body fat mass, (e) body lean mass and (f) body weight were monitored throughout the study. (g) Body composition of mice before (day −6) and after (day 7) treatment with PBS or FGF21. White circles, −CH:PBS; black squares, +CH:PBS; grey triangles, +CH:FGF21. Data are presented as mean ± SEM (n = 6–7/group). Differences compared with +CH:PBS group were calculated by two-way ANOVA (a, d, e, f) or one-way ANOVA (c, g). Differences between PBS and FGF21 groups were analysed using a two-tailed t test (b). *^,‡ p < 0.05, **^,‡‡ p < 0.01 (*, +CH:PBS vs −CH:PBS; ^‡, +CH:PBS vs +CH:FGF21)