Table 2.
Adipose expression of candidate genes in loci associated with FSI and their association with clinical and adipose variables
| Chr | Trait | SNP | Gene | BMI | FSI | FPG | Basal lipolysis | Insulin-stimulated lipogenesis | WAT morphology | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p value | R | p value | R | p value | R | p value | R | p value | R | p value | R | ||||
| 1 | FSI | rs2820436 | LYPLAL1 a | 1.2 × 10−4b | – | 1.1 × 10−2b | – | 4.4 × 10−2 | – | 2.6 × 10−2 | – | ||||
| FSIadjBMI | rs4846565 | EPRS | 1.6 × 10−3b | + | 1.5 × 10−5b,c | + | 4.8 × 10−3b | – | |||||||
| IARS2 | 1.3 × 10−4b | – | 4.0 × 10−3b | – | 2.0 × 10−2 | – | |||||||||
| RAB3GAP2 | 8.7 × 10−4b | – | 8.4 × 10−3b | – | 7.1 × 10−3b | – | 1.9 × 10−4b,c | – | 1.0 × 10−2 | – | |||||
| 2 | FSI | rs1530559 | R3HDM1 | 1.5 × 10−5a,c | + | ||||||||||
| UBXN4 | 9.7 × 10−a | – | |||||||||||||
| MCM6 | 2.2 × 10−2 | – | 2.4 × 10−3b,c | – | 3.6 × 10−2 | – | |||||||||
| DARS | 3.6 × 10−12b | – | 3.9 × 10−8b | – | 2.0 × 10−3b | – | 9.3 × 10−4b | – | 6.9 × 10−4b | + | 3.2 × 10−2 | – | |||
| CXCR4 | 6.9 × 10−5b | + | 1.1 × 10−3b | + | 3.3 × 10−2 | + | 2.2 × 10−2 | + | 2.7 × 10−2 | – | 4.4 × 10−3 | + | |||
| 2 | FSI | rs10195252 | GRB14 a | 1.1 × 10−2b | + | 2.9 × 10−2 | + | 3.5 × 10−4b,c | – | ||||||
| FSIadjBMI | CSRNP3 | 1.4 × 10−3b | + | 3.3 × 10−2 | + | 4.0 × 10−2 | – | ||||||||
| GALNT3 | 1.3 × 10−2b | + | |||||||||||||
| TTC21B | 1.3 × 10−8b | – | 1.0 × 10−6b | – | 4.2 × 10−5b,c | – | 7.5 × 10−4b | – | 8.1 × 10−3 | – | |||||
| SCN1A | 3.1 × 10−3b | – | 3.1 × 10−2 | – | 3.6 × 10−2 | – | |||||||||
| 2 | FSI | rs2972143 | IRS1 a | 1.8 × 10−7b | – | 2.8 × 10−5b | – | 2.6 × 10−3b | – | 3.4 × 10−4b | + | 3.3 × 10−2 | – | ||
| FSIadjBMI | rs2943645 | RHBDD1 | 1.3 × 10−2b | + | 3.0 × 10−3b | + | 1.0 × 10−2 | + | |||||||
| MFF | 2.3 × 10−3b | – | |||||||||||||
| AGFG1 | 1.7 × 10−8b | + | 6.9 × 10−8b,c | + | 3.9 × 10−3b | + | 7.9 × 10−4b | + | 2.7 × 10−3b | – | 2.5 × 10−2 | + | |||
| 3 | FSIadjBMI | rs17036328 | PPARG a | 7.1 × 10−15b | – | 3.9 × 10−11b,c | – | 3.5 × 10−3b | – | 8.3 × 10−6b,c | – | 5.7 × 10−6b,c | + | 2.8 × 10−3 | – |
| SYN2 | 1.1 × 10−3b | – | 1.2 × 10−3b | – | |||||||||||
| TSEN2 | 2.8 × 10−5b | – | 1.2 × 10−3b | – | 4.5 × 10−2 | – | 1.3 × 10−3b | – | 2.0 × 10−2 | + | 3.0 × 10−3 | – | |||
| RAF1 | 6.9 × 10−4b | + | 1.2 × 10−3b | + | 6.0 × 10−4b,c | – | |||||||||
| TMEM40 | 3.6 × 10−4b,c | + | |||||||||||||
| 4 | FSIadjBMI | rs3822072 | FAM13A a | 2.2 × 10−15b | – | 5.6 × 10−12b,c | – | 6.7 × 10−4b | – | 5.6 × 10−7b,c | – | 1.6 × 10−2 | + | 8.2 × 10−4b | – |
| GPRIN3 | 1.0 × 10−9b | + | 6.3 × 10−6b | + | 3.7 × 10−2 | + | 3.5 × 10−3b | – | |||||||
| SNCA | 5.8 × 10−3b | + | 9.6 × 10−3b | + | 1.4 × 10−3b | + | 8.2 × 10−3 | + | |||||||
| MMRN1 | 1.4 × 10−3b | + | 2.2 × 10−3b | + | 3.0 × 10−2 | + | |||||||||
| CCSER1 | 1.1 × 10−2b | + | 1.6 × 10−2 | – | |||||||||||
| 4 | FSI | rs9884482 | INTS12 | 3.3 × 10−2 | – | 8.8 × 10−3b | – | 3.5 × 10−2 | – | ||||||
| FSIadjBMI | rs974801 | GSTCD | 7.3 × 10−3b | + | |||||||||||
| TBCK | 1.6 × 10−2b | – | 2.8 × 10−2 | – | 5.8 × 10−3b | – | |||||||||
| AIMP1 | 4.2 × 10−5b | – | 7.9 × 10−4b | – | 1.3x10−3b | – | 6.2 × 10−2 | – | 2.7 × 10−2 | + | |||||
| 4 | FSIadjBMI | rs6822892 | FAM198B | 3.3 × 10−8b | + | 1.4 × 10−7b,c | + | 3.8 × 10−3b | + | 2.1 × 10−2 | + | 4.7 × 10−3b | – | 6.1 × 10−3 | + |
| 5 | FSI | rs4865796 | ARL15 a | 1.5 × 10−5b | + | 1.0 × 10−4b | + | 2.4 × 10−2 | + | 1.8 × 10−6b,d | – | 5.2 × 10−3 | + | ||
| FSIadjBMI | ITGA2 | 2.1 × 10−5b | + | 5.1 × 10−5b | + | 1.3 × 10−2 | + | 3.0 × 10−2 | + | 6.4 × 10−3b,c | – | ||||
| MOCS2 | 1.1 × 10−5b | – | 1.3 × 10−3b | – | 5.2 × 10−3b | – | 4.6 × 10−2 | – | |||||||
| NDUFS4 | 2.0 × 10−8b | – | 2.4 × 10−7b | – | 4.0 × 10−2 | – | 2.2 × 10−4b | + | 5.9 × 10−5b | – | |||||
| 5 | FSIadjBMI | rs459193 | MAP3K1 a | 5.8 × 10−9b | + | 2.9 × 10−8b,c | + | 1.0 × 10−3b | + | 2.9 × 10−3b | + | 7.2 × 10−5b | – | 3.7 × 10−2 | + |
| SKIV2L2 | 1.9 × 10−6b | – | 1.5 × 10−5b | – | 3.3 × 10−3 | – | 5.5 × 10−4b | – | 1.4 × 10−2 | – | |||||
| 6 | FSIadjBMI | rs6912327 | UHRF1BP1 a | 1.1 × 10−2 | – | ||||||||||
| NUDT3 | 2.9 × 10−5b | + | 2.0 × 10−8b,c | + | 1.4 × 10−3b | + | 8.9 × 10−3 | + | 2.6 × 10−2 | – | |||||
| RPS10 | 1.0 × 10−2b | – | 4.2 × 10−3 | – | |||||||||||
| C6orf106 | 1.2 × 10−5b | + | 3.4 × 10−4b | + | 3.2 × 10−2 | + | 2.1 × 10−3b | + | 3.1 × 10−2 | + | |||||
| TCP11 | 6.0 × 10−3b | – | 1.8 × 10−2b | – | |||||||||||
| SCUBE3 | 2.4 × 10−3b,c | – | |||||||||||||
| 6 | FSI | rs2745353 | RNF146 | 2.6 × 10−6b | – | 8.7 × 10−4b | – | 6.2 × 10−3b | – | 3.0 × 10−3b | – | 8.6 × 10−3 | – | ||
| ECHDC1 | 2.5 × 10−2 | – | 1.7 × 10−3a | + | |||||||||||
| THEMIS | 5.1 × 10−5b | + | 7.0 × 10−5b | + | 7.5 × 10−3b | + | 5.6 × 10−3b | – | |||||||
| 7 | FSI | rs1167800 | GTF2I | 8.5 × 10−6b | – | 9.0 × 10−4b | – | 3.2 × 10−2 | – | 2.4 × 10−4b | – | ||||
| NCF1 | 5.2 × 10−4b | + | 2.0 × 10−3b | + | 2.9 × 10−3b | + | 1.8 × 10−2 | + | |||||||
| STAG3L2 | 2.3 × 10−4b | – | 2.0 × 10−2b | – | 3.3 × 10−4b | + | 1.3 × 10−2 | – | |||||||
| GATSL2 | 4.3 × 10−9b | + | 2.5 × 10−10b,c | + | 3.8 × 10−3b | + | 1.7 × 10−3b | – | 2.1 × 10−3 | + | |||||
| SPDYE8P | 3.5 × 10−3b | – | 2.4 × 10−4b,c | – | 1.0 × 10−3b | – | |||||||||
| TRIM73 | 1.5 × 10−2b | – | |||||||||||||
| POM121C | 1.1 × 10−3b | – | 2.0 × 10−2b | – | 3.4 × 10−3b | + | 2.6 × 10−2 | – | |||||||
| PMS2P3 | 8.0 × 10−3 | – | 4.8 × 10−2 | – | |||||||||||
| 8 | FSIa | rs983309 | PPP1R3B a | 2.9 × 10−3b | – | ||||||||||
| FSIadjBMI | rs2126259 | CLDN23 | 4.9 × 10−3b | – | 1.0 × 10−2b | – | 2.9 × 10−3b | – | 1.2 × 10−2 | – | |||||
| MFHAS1 | 3.0 × 10−11b | + | 5.1 × 10−6b | + | 3.7 × 10−2 | + | 2.4 × 10−4b | – | |||||||
| ERI1 | 4.1 × 10−5b | + | 1.4 × 10−3b | + | 2.1 × 10−2 | – | |||||||||
| TNKS | 1.1 × 10−2b | – | 6.2 × 10−2 | – | |||||||||||
| 10 | FSI | rs7903146 | TCF7L2 a | 1.2 × 10−3b | – | 4.2 × 10−2 | – | 2.1 × 10−2 | – | 2.4 × 10−2 | – | 3.6 × 10−3 | – | ||
| ABLIM1 | 1.7 × 10−5b | + | 1.2 × 10−3b | + | 3.3 × 10−2 | + | 3.1 × 10−2 | + | 2.4 × 10−6b | – | |||||
| 16 | FSI | rs1421085 | FTO c | 7.2 × 10−3b | + | ||||||||||
| RBL2 | 2.7 × 10−5b | – | 4.0 × 10−2 | – | 3.3 × 10−2 | – | 2.8 × 10−4b | – | 9.8 × 10−3 | – | |||||
| RPGRIP1L | 2.4 × 10−5b | + | 1.5 × 10−6b,c | + | 3.4 × 10−2 | + | |||||||||
| IRX3 | 1.0 × 10−3b | – | 9.4 × 10−3b | – | 1.1 × 10−2 | – | |||||||||
| 19 | FSI | rs731839 | PEPD a | 1.6 × 10−6b | + | 1.5 × 10−3b | + | 2.4 × 10−2 | + | 1.3 × 10−2 | + | ||||
| FSIadjBMI | KCTD15 | 7.1 × 10−3b | + | ||||||||||||
Microarray results from the clinical cohort were analysed by regression in QLUCORE, adjusting for array batch, and for WAT morphology, adjusting for age. Only results with p < 0.05 are shown
aOriginal candidate gene from Scott et al [11]
bFDR <5%
cNominally significant after adjustment for BMI in QLUCORE
R, regression coefficient, which can be either positive (+) or negative (−)