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. 2019 Apr 4;8:e43944. doi: 10.7554/eLife.43944

Figure 4. ANAC044 and ANAC085 are not required for HR-mediated DNA repair.

Figure 4.

(A) Transcriptional response of DNA repair-related genes to bleomycin. Five-day-old seedlings of WT, sog1-101 and anac044-1 anac085-1 were treated with or without 0.6 µg/ml bleomycin for 10 hr. Total RNA was extracted from root tips and subjected to microarray analysis. Purple and green colours indicate up- and down-regulation, respectively, of genes by bleomycin treatment. (B) HR assay. The GUS reporter constructs before and after HR are shown (upper panel). Two-week-old plants of WT, sog1-101 and anac044-1 anac085-1 carrying the GUS reporter construct were irradiated with or without gamma rays (50 Gy), and grown for 3 days. Numbers of blue spots on leaves were counted. Data are presented as mean ± SD (n = 50). Different letters indicate significant differences between samples (Student’s t-test, p<0.05).

Figure 4—source data 1. Source data.
elife-43944-fig4-data1.xlsx (30.6KB, xlsx)
DOI: 10.7554/eLife.43944.017