Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (S. cerevisiae) |
BY4741 | (Huh et al., 2003) | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | |
| Strain, strain background (S. cerevisiae) |
BY4742 | (Huh et al., 2003) | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | |
| Strain, strain background (S. cerevisiae) |
BY4741-Reb1 | Constructed in this study |
MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 REB1–GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4741-HHT2 | Constructed in this study |
MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 HHT2–GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4741–Sth1 | Constructed in this study |
MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 STH1–GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4741–NHP6A | Constructed in this study |
MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NHP6A–GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4742–Reb1 | Constructed in this study |
MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 REB1–GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4742-HHT2 | Constructed in this study |
MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 HHT2–GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4742–Sth1 | Constructed in this study |
MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 Sth1–GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4742–NHP6a | Constructed in this study |
MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 NHP6A–GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4743–Reb1 | Constructed in this study |
MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 REB1–GFP::His3MX/Factor-GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4743–HHT2 | Constructed in this study |
MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 HHT2–GFP::His3MX/Factor-GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4743–Sth1 | Constructed in this study |
MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 Sth1–GFP::His3MX/Factor-GFP::His3MX | See 'Materials and methods: FRAP assay' |
| Strain, strain background (S. cerevisiae) |
BY4743–NHP6A | Constructed in this study |
MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 NHP6A–GFP::His3MX/Factor-GFP::His3MX | See 'Materials and methods' |