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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Acta Neuropathol. 2019 Jan 28;137(3):437–454. doi: 10.1007/s00401-019-01959-4

Multiple system atrophy prions retain strain specificity after serial propagation in two different Tg(SNCA*A53T) mouse lines

Amanda L Woerman 1,2,*, Abby Oehler 1, Sabeen A Kazmi 1, Jisoo Lee 1, Glenda M Halliday 3, Lefkos T Middleton 4, Steve M Gentleman 5, Daniel A Mordes 6, Salvatore Spina 2, Lea T Grinberg 2, Steven H Olson 1,2, Stanley B Prusiner 1,2,7
PMCID: PMC6454887  NIHMSID: NIHMS1519813  PMID: 30690664

Abstract

Previously, we reported that intracranial inoculation of brain homogenate from multiple system atrophy (MSA) patient samples produces neurological disease in the transgenic (Tg) mouse model TgM83+/−, which uses the prion protein promoter to express human α-synuclein harboring the A53T mutation found in familial Parkinson’s disease (PD). In our studies, we inoculated MSA and control patient samples into Tg mice constructed using a P1 artificial chromosome to express wild-type (WT), A30P, and A53T human α-synuclein on a mouse α-synuclein knockout background [Tg(SNCA+/+)Nbm, Tg(SNCA*A30P+/+)Nbm, and Tg(SNCA*A53T+/+)Nbm]. In contrast to studies using TgM83+/− mice, motor deficits were not observed by 330 to 400 days in any of the Tg(SNCA)Nbm mice after inoculation with MSA brain homogenates. However, using a cell-based bioassay to measure α-synuclein prions, we found brain homogenates from Tg(SNCA*A53T+/+)Nbm mice inoculated with MSA patient samples contained α-synuclein prions, whereas control mice did not. Moreover, these α-synuclein aggregates retained the biological and biochemical characteristics of the α-synuclein prions in MSA patient samples. Intriguingly, Tg(SNCA*A53T+/+)Nbm mice developed α-synuclein pathology in neurons and astrocytes throughout the limbic system. This finding is in contrast to MSA-inoculated TgM83+/− mice, which develop exclusively neuronal α-synuclein aggregates in the hindbrain that cause motor deficits with advanced disease. In a crossover experiment, we inoculated TgM83+/− mice with brain homogenate from two MSA patient samples or one control sample first inoculated, or passaged, in Tg(SNCA*A53T+/+)Nbm animals. Additionally, we performed the reverse experiment by inoculating Tg(SNCA*A53T+/+)Nbm mice with brain homogenate from the same two MSA samples and one control sample first passaged in TgM83+/− animals. The TgM83+/− mice inoculated with mouse-passaged MSA developed motor dysfunction and α-synuclein prions, whereas the mouse-passaged control sample had no effect. Similarly, the mouse-passaged MSA samples induced α-synuclein prion formation in Tg(SNCA*A53T+/+)Nbm mice, but the mouse-passaged control sample did not. The confirmed transmission of α-synuclein prions to a second synucleinopathy model and the ability to propagate prions between two distinct mouse lines while retaining strain-specific properties provides compelling evidence that MSA is a prion disease.

Keywords: α-synuclein, neurodegeneration, proteinopathies, transmission models

Introduction

Multiple system atrophy (MSA) is a sporadic neurodegenerative disorder characterized by progressive autonomic and motor dysfunction. The term MSA was introduced in 1969 after Graham and Oppenheimer compared brain tissue from patients with olivopontocerebellar atrophy (OPCA), Shy–Drager syndrome, and striatonigral degeneration (SND), and concluded the three disorders were distinct clinical presentations of the same neuropathogenic process [8]. The subsequent identification of Papp–Lantos bodies, or glial cytoplasmic inclusions (GCIs), in oligodendrocytes of OPCA, Shy–Drager syndrome, and SND patients supported this reclassification [28]. In 1998, two independent groups showed that the protein α-synuclein, which was linked to Parkinson’s disease (PD) one year prior, aggregated to form GCIs in brain sections from deceased MSA patients [37, 39].

In the twenty years that followed this discovery, substantial work focused on developing transgenic (Tg) mouse models of α-synuclein misfolding and accumulation to better understand disease etiology and progression in the synucleinopathies, including PD and MSA. Mouse models overexpressing either wild-type (WT) or mutant human α-synuclein (SNCA) were created using central nervous system (CNS)–specific promoters to localize α-synuclein expression either in neurons to model PD [47, 11, 13, 18, 21, 23, 33, 35, 38, 46] or in oligodendrocytes to model MSA [14, 36, 44]. In the oligodendrocyte-specific models, spontaneous accumulation of α-synuclein was used to investigate neuroinflammation, demyelination, loss of dopaminergic neurons, and motor dysfunction in disease progression. These models were also used to identify and evaluate a vaccine to treat MSA in pre-clinical testing [22]. However, it remains unknown if the conformation of misfolded α-synuclein that develops in these mice is consistent with the conformation present in an MSA patient. Without this critical bridge, drug candidates may fail in clinical trials with MSA patients.

To meet this challenge, several studies have recently shown that brain homogenates from deceased MSA patient samples transmit neurological disease ~120 days post-inoculation (dpi) into the hemizygous TgM83+/− mouse model [31, 4043]. TgM83+/− mice, which use the mouse prion protein promoter to express human α-synuclein with the A53T mutation [5], do not develop spontaneous disease [40]. However, inoculating these animals with MSA patient samples induces the accumulation of neuronal α-synuclein aggregates that are insoluble and hyperphosphorylated [31, 40]. Testing the biological and biochemical properties of the α-synuclein aggregates that develop in TgM83+/− mice showed that the resulting inclusions retain key characteristics of α-synuclein aggregates isolated from human MSA patient samples [41]. These findings argue that in MSA, α-synuclein misfolds into a self-templating conformation and becomes a prion.

In addition to the TgM83+/− mouse model, we report here attempts to transmit MSA to three Tg mouse models that express WT [Tg(SNCA+/+)Nbm] or mutant α-synuclein [Tg(SNCA*A30P+/+)Nbm and Tg(SNCA*A53T+/+)Nbm]. The three Tg(SNCA)Nbm models were created using a P1 artificial chromosome, resulting in more widespread transgene expression, with the mouse α-synuclein gene knocked out [17]. Following inoculation with MSA patient samples, α-synuclein prions were only detected in asymptomatic Tg(SNCA*A53T+/+)Nbm mice terminated 330 dpi. Similar to our previous observations with TgM83+/− mice, these aggregates retained biological and biochemical properties of α-synuclein prions isolated from MSA patient samples, demonstrating Tg(SNCA*A53T+/+)Nbm mice also propagate MSA prions with high fidelity. Notably, α-synuclein pathology in these animals accumulates in neurons and astrocytes throughout the limbic system in contrast to the exclusively neuronal hindbrain aggregates that contribute to autonomic and motor symptoms in TgM83+/− mice.

In serial passaging studies, brain homogenates from Tg(SNCA*A53T+/+)Nbm mice inoculated with two MSA patient samples induced neurological disease in TgM83+/− animals. While serially passaging the control patient sample had no effect in the mice, the passaged MSA samples caused neuronal α-synuclein inclusions to form throughout the hindbrain. In the reverse experiment, brain homogenates from TgM83+/− mice inoculated with the same MSA patient samples induced α-synuclein prion propagation in Tg(SNCA*A53T+/+)Nbm mice. These animals developed α-synuclein inclusions in the limbic system, whereas passaging studies with the control patient sample had no effect. Importantly, α-synuclein prions isolated from both mouse models showed similar biological activities and biochemical stabilities to α-synuclein prions isolated from MSA patient samples. These studies demonstrate that α-synuclein prions not only can propagate in two different Tg mouse models but also can be passaged from one model into the other while retaining MSA-specific strain characteristics. Our studies reported here extend earlier findings arguing that α-synuclein prions cause MSA.

Materials and Methods

Human tissue samples

Frozen brain tissue samples from neuropathologically confirmed cases of MSA and PD were provided by the Sydney Brain Bank, the Parkinson’s UK Tissue Bank at Imperial College, and the Massachusetts Alzheimer’s Disease Research Center. Fixed and frozen brain tissue from two neuropathologically confirmed cases of MSA were provided by the Neurodegenerative Disease Brain Bank at the University of California, San Francisco. Control patient tissue was provided by Dr. Martin Ingelsson (Uppsala University) and Dr. Deborah Mash (Miami Brain Bank). Demographic information about samples used is included in Online Resource, Table S1.

Mice

Animals were maintained in an AAALAC-accredited facility in compliance with the Guide for the Care and Use of Laboratory Animals. All procedures used in this study were approved by the University of California, San Francisco, Institutional Animal Care and Use Committee. All animals were maintained under standard environmental conditions with a 12:12-h light:dark cycle and free access to food and water. The dbl-PAC-Tg(SNCAWT)+/+;Snca−/−, dbl-PAC-Tg(SNCAA30P)+/+;Snca−/−, and dbl-PAC-Tg(SNCAA53T)+/+;Snca−/− [referred to here as Tg(SNCA+/+)Nbm, Tg(SNCA*A30P+/+)Nbm, and Tg(SNCA*A53T+/+)Nbm, respectively] were provided by Robert L. Nussbaum. To improve litter viability, all Tg(SNCA)Nbm mouse pups used in these studies were fostered using CD-1 females purchased from The Jackson Laboratory (USA). TgM83+/− mice were generated by breeding TgM83+/+ mice [5], purchased from The Jackson Laboratory, with B6C3F1 mice.

Inoculations

Fresh frozen human tissue and frozen mouse half-brains were used to create a 10% (wt/vol) homogenate using calcium- and magnesium-free 1× DPBS using the Omni Tissue Homogenizer (Omni International). Homogenates were diluted to 1% using 5% (wt/vol) bovine serum albumin in 1× DPBS.

Eight-week-old Tg(SNCA+/+)Nbm, Tg(SNCA*A30P+/+)Nbm, and Tg(SNCA*A53T+/+)Nbm mice were anesthetized with isoflurane prior to inoculation. Free-hand inoculations with human patient samples were performed using 30 μL of the 1% brain homogenate in the thalamus. Following inoculation, all mice were assessed twice a week for the onset of neurological signs based on standard diagnostic criteria for prion disease [2]. Uninoculated Tg(SNCA+/+)Nbm mice were euthanized at 500 days of age. Uninoculated Tg(SNCA*A30P+/+)Nbm mice were euthanized between 450–500 days of age. Uninoculated Tg(SNCA*A53T+/+)Nbm mice were euthanized between 450–625 days of age. Tg(SNCA+/+)Nbm mice were euthanized 400 dpi, and Tg(SNCA*A30P+/+)Nbm and Tg(SNCA*A53T+/+)Nbm mice were collected 330 dpi. Tg(SNCA*A53T+/+)Nbm mice inoculated with brain homogenate from TgM83+/− mice inoculated with either MSA or control patient samples were euthanized 300 dpi. Ten-week-old TgM83+/− mice inoculated with brain homogenate from Tg(SNCA*A53T+/+)Nbm mice inoculated with MSA patient samples were euthanized after developing progressive neurological signs, or 450 dpi. Additionally, mice inoculated with brain homogenate from Tg(SNCA*A53T+/+)Nbm mice inoculated with a control patient sample were euthanized 450 dpi. Following euthanasia, the brain was removed and bisected down the midline. The left hemisphere was frozen for biochemical analysis, and the right hemisphere was fixed in formalin for neuropathological assessment.

Alpha-synuclein prion quantification assay

A 10% (wt/vol) brain homogenate was prepared using frozen human tissue in calcium- and magnesium-free 1× DPBS using the Omni Tissue Homogenizer with disposable soft tissue tips (Omni International). Aggregated protein was isolated from the patient samples using sodium phosphotungstic acid (PTA) (Sigma) as described [34, 43]. Isolated protein pellets were diluted 1:10 in 1× DPBS before testing in the α-synuclein prion quantification assays.

HEK293T cells expressing α-syn140–YFP, α-syn140*E46K–YFP, α-syn140*A53T–YFP, α-syn140*A30P,A53T–YFP, α-syn140*E46K,A53T–YFP, α-syn95*A53T–YFP, and α-syn97*A53T–YFP were previously reported, and culture and assay conditions were used as described [41]. Images of cells incubated with Tg mouse samples were collected using the IN Cell Analyzer 6000 (GE Healthcare). DAPI and FITC channels were used to collect two images from five different regions in each well. Each set of images was analyzed using the IN Cell Developer software with an algorithm created to detect intracellular aggregates in living cells, quantified as total fluorescence per cell (× 103, arbitrary units, A.U.).

Immunohistochemistry and neuropathology

Mouse brains were fixed in 10% (vol/vol) formalin and cut into four sections prior to processing through graded alcohols, clearing with xylene, infiltrating with paraffin, and embedding. Thin slices (8 μm) were cut, collected on slides, deparaffinized, and exposed to heat-mediated antigen retrieval with citrate buffer (0.1 M, pH 6) for 20 min. Slides were stained using a Thermo Fisher 480S Autostainer with 30 min blocking in 10% (vol/vol) normal goat serum and incubation with primary antibody (2 h) and secondary antibody (2 h). Primary antibodies used include EP1536Y (pS129 α-synuclein; 1:1,000; Abcam), MABN826 (pS129 α-synuclein; 1:250; Millipore), glial fibrillary acidic protein (GFAP; 1:500; Abcam), ionized calcium binding adaptor molecule 1 (Iba1; 1:250; Synaptic Systems), microtubule associated protein 2 (MAP2; 1:500; Abcam), and oligodendrocyte transcription factor 2 (Olig2; 1:500; Millipore). Secondary antibodies conjugated to AlexaFluor 488, 568, or 647 (Thermo Fisher) were used. Staining for co-localization of α-synuclein with neural/glial antigen 2 (NG2)–positive cells was done overnight at room temperature using MABN826 (pS129 α-synuclein; 1:250; Millipore) and NG2 (1:300; Millipore) after autoclaving at 105 ºC for 10 min.

Bielschowsky’s silver staining was done using deparaffinized sections. Slides were incubated in 40 ºC 20% (wt/vol) silver nitrate (Thermo Fisher) for 16 min before 3 washes in distilled H2O. Ammonium hydroxide (Acros Organics) was added to the silver nitrate solution until the precipitate formed was clear. Slides were incubated in the ammonium silver solution for 30 min at 40 ºC before moving to developer working solution for 1 min. Developer working solution was made fresh by adding 8 drops concentrated ammonium hydroxide and 8 drops developer stock solution to 50 mL distilled H2O. Developer stock solution was made fresh by adding 20 mL of 37–40% formaldehyde (Sigma), 0.5 g sodium citrate tribasic dihydrate (Sigma), and 2 drops 70% nitric acid (Sigma) to 100 mL distilled H2O. Slides were dipped in 1% (vol/vol) ammonium hydroxide solution for 1 min before washing 3 times in distilled H2O. The slides were incubated in 5% (wt/vol) sodium thiosulfate solution for 5 min before again washing 3 times in distilled H2O. Finally, slides were dehydrated through graded alcohols and cleared with xylene before mounting.

Slides were imaged using the Zeiss AxioScan.Z1. Digital images were analyzed using the Zen Analysis software package (Zeiss). To quantify α-synuclein neuropathology, a pixel intensity threshold was determined using a positive control slide and was then applied to all slides. Regions of interest were drawn around the caudoputamen, hippocampus and fimbria, piriform cortex and amygdala, sensory cortex, thalamus, hypothalamus, parahippocampal cortex, and pons in the Tg(SNCA)Nbm mice. Regions of interest were drawn around the hippocampus, thalamus, hypothalamus, midbrain, and pons in the TgM83+/− mice. The regions selected for each model were based on the distribution of neuropathology specific to the Tg mouse line. The percentage of pixels positive for staining in each region was determined.

Guanidine hydrochloride denaturation

Guanidine hydrochloride (GdnHCl) denaturation of brain homogenates was performed as previously reported [41]. The resulting protein pellets were resuspended in 40 μL 1× NuPAGE LDS loading buffer and were boiled for 10 min prior to immunoblotting.

Immunoblotting

Brain homogenates from 2-month-old TgM83+/−, Tg(SNCA+/+)Nbm, Tg(SNCA*A30P+/+)Nbm, and Tg(SNCA*A53T+/+)Nbm mice were clarified by centrifuging for 5 min at 1,000 × g. The supernatant was collected and total protein was measured using the bicinchoninic acid assay (Pierce), and samples were adjusted to 1 mg/mL total protein. Samples were loaded onto a 10% Bolt Bis-Tris gel, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed using the MES buffer system. Protein was transferred to a nitrocellulose membrane. The membrane was blocked in Odyssey blocking buffer (LiCor) for 1 h at room temperature. Incubation with anti-vinculin (1:10,000; Abcam) and Clone 42 (1:1,000; BD Biosciences) was in Odyssey block buffer with 0.2% Tween 20 overnight at 4 °C. Membranes were washed three times with 1× Tris-buffered saline with Tween 20 (TBST) before incubating with near-infrared secondary antibodies (IRDye Goat anti-Mouse-680RD and IRDye Goat anti-Rabbit-800CW, respectively; 1:20,000; LiCor) diluted in Odyssey block buffer with 0.2% Tween 20 and 0.1% SDS for 1 h at room temperature. After washing the blot three times in 1× TBST, the membrane was washed a final time in 1× TBS prior to imaging and analysis using the Odyssey Fc (LiCor). Blots were analyzed using Image Studio software (LiCor), using the TgM83+/− bands to standardize the data.

GdnHCl samples were loaded onto a 10% Novex Bis-Tris gel (Thermo Fisher). SDS-PAGE was performed using the MES buffer system. Protein was transferred to a nitrocellulose membrane. Membranes were blocked in blocking buffer [5% (wt/vol) nonfat milk in 1× Tris-buffered saline containing 0.05% (vol/vol) Tween 20 (TBST)] for 1 h at room temperature. Primary antibody incubation (EP1536Y; 1:4,000; Abcam) was in blocking buffer overnight at 4 °C. Membranes were washed three times with 1× TBST before incubating with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (1:10,000; Bio-Rad) diluted in blocking buffer for 1 h at room temperature. After washing blots three times in 1× TBST, membranes were developed using the enhanced chemiluminescent detection system (GE Healthcare) for exposure to X-ray film. Blots were analyzed via ImageJ, using the 0 M guanidine hydrochloride (GnHCl) sample to standardize the data.

Statistical analysis

Data are presented as mean ± standard deviation. Analysis of data collected from the α-syn140*A53T–YFP cell assay measuring spontaneous α-synuclein prion formation was done using a Kruskal–Wallis test with a Dunn’s multiple comparison post-hoc test. Statistical analysis of data collected from the α-syn140*A53T–YFP cell assay comparing control- and MSA-inoculated Tg(SNCA)Nbm mice was done by averaging values from mice inoculated with each sample. The average for each of the five control samples and the five MSA samples used in each Tg mouse line were compared using a Mann–Whitney test. The same analysis was performed comparing Tg(SNCA*A53T+/+)Nbm mice inoculated with control or PD patient samples. Mouse neuropathology was analyzed using a two-tailed Student’s t-test with unequal variance to compare MSA-inoculated mice that contained α-synuclein prions with C2-inoculated mice by brain region. Kaplan–Meier curves in the TgM83+/− mice were analyzed using a log-rank Mantel–Cox test. Comparison of α-syn140*A53T–YFP cell assay data from the serial passaging studies in the TgM83+/− and Tg(SNCA*A53T+/+)Nbm mice was analyzed using a one-way ANOVA with a Dunnett multiple comparison post-hoc test. Significance was determined with a P-value < 0.05.

Results

Tg(SNCA)Nbm mice do not develop spontaneous α-synuclein prions

Tg(SNCA)Nbm mice are homozygous for either WT or mutant (A30P or A53T) α-synuclein and are maintained on an endogenous α-synuclein knockout background (Snca−/−) [17]. These animals were reported to develop robust alterations in the enteric nervous system, including accumulation of α-synuclein aggregates resistant to proteinase K. Interestingly, while we were previously unable to induce neurological disease in Tg(SNCA+/+)Nbm mice following inoculation with brain homogenate from two MSA patient samples [31], others reported that inoculation of sarkosyl-insoluble preparations from MSA patient samples induced widespread α-synuclein neuropathology in these mice 9 months post-inoculation [1]. Comparing the three Tg(SNCA)Nbm mouse models with TgM83+/− mice [5], which develop neurological disease ~120 days after MSA inoculation [31, 40, 42], we measured total α-synuclein expression in the brains of 2-month-old TgM83+/−, Tg(SNCA+/+)Nbm, Tg(SNCA*A30P+/+)Nbm, and Tg(SNCA*A53T+/+)Nbm animals (Online Resource, Figs. S1a, b). Among the four models, hemizygous TgM83 mice express the most human α-synuclein while Tg(SNCA*A53T+/+)Nbm mice express the least. Fixed brain sections from aged Tg(SNCA)Nbm mice (>450 days old) assessed for accumulation of phosphorylated α-synuclein neuropathology showed these animals do not develop spontaneous inclusions (EP1536Y primary antibody; Online Resource, Fig. S1c). Frozen tissue from the same mice was homogenized and tested for α-synuclein prion formation using the α-syn140*A53T–YFP cell assay [43] (Online Resource, Fig. S1d). In this assay, human embryonic kidney (HEK) cells are used to express full-length human α-synuclein with the A53T mutation fused to yellow fluorescent protein (YFP). Samples containing α-synuclein prions infect the cells and induce YFP-positive inclusions, indicated by the dotted line at 10 × 103 arbitrary units (A.U.). None of the three aged Tg(SNCA)Nbm models developed spontaneous α-synuclein prions (WT: 1.6 ± 0.5; A30P: 1.7 ± 0.4; A53T: 1.6 ± 0.5 A.U.).

Tg(SNCA*A53T+/+) mice propagate MSA prions

Eight-week-old Tg(SNCA+/+)Nbm, Tg(SNCA*A30P+/+)Nbm, and Tg(SNCA*A53T+/+)Nbm mice were inoculated with brain homogenate from control (C2, C3, C14, C16, and C17) or MSA (MSA6, MSA13, MSA16, MSA17, and MSA18) patient samples (Table 1; Fig. 1a; and Online Resource, Table S2). Unlike TgM83+/− mice, which develop neurological disease ~120 days after MSA inoculation, the Tg(SNCA)Nbm animals remained asymptomatic for >330 dpi. Tg(SNCA+/+)Nbm mice were euthanized 400 dpi while Tg(SNCA*A30P+/+)Nbm and Tg(SNCA*A53T+/+)Nbm mice were euthanized 330 dpi. The left hemisphere of the brain was collected frozen from each animal, and a 10% brain homogenate was prepared. Alpha-synuclein aggregates were isolated by PTA precipitation [34], and the resulting protein pellets were tested for infectivity using the α-syn140*A53T–YFP cell assay (Table 2). Alpha-synuclein isolated from the Tg(SNCA+/+)Nbm mice inoculated with either control or MSA patient samples did not infect the cells (P > 1). Additionally, only one Tg(SNCA*A30P+/+)Nbm mouse inoculated with patient sample MSA18 infected the α-syn140*A53T–YFP cells (P = 0.31). However, brain homogenate from at least half of the Tg(SNCA*A53T+/+)Nbm mice inoculated with each of the MSA patient samples infected the α-syn140*A53T–YFP cells, demonstrating that MSA prions can propagate in A53T-expressing mice (P < 0.01).

Table 1.

Efficiency of MSA transmission to Tg(SNCA) mice.

Patient Sample TgM83+/− Tg(SNCA+/+)Nbm Tg(SNCA*A30P+/+)Nbm Tg(SNCA*A53T+/+)Nbm
F M n/n0 F M n/n0 F M n/n0 F M n/n0
C2 8 4 0/12 4 3 0/7 4 4 0/8 4 3 0/7
C3 - - n.d. 4 4 0/8 4 4 0/8 4 4 0/8
C14 0 8 0/8 3 4 0/7 4 4 0/8 4 4 0/8
C16 - - n.d. 4 4 0/8 4 4 0/8 4 4 0/8
C17 - - n.d. 4 4 0/8 4 4 0/8 4 4 0/8
MSA6 4 4 8/8 4 4 0/8 4 4 0/8 4 3 7/7
MSA13 3 5 8/8 3 4 0/7 4 4 0/8 4 4 7/8
MSA16 - - n.d. 2 4 0/6 4 3 0/7 3 4 4/7
MSA17 6 3 9/9 4 4 0/8 4 4 0/8 4 4 2/8
MSA18 4 4 8/8 4 4 0/8 4 4 1/8 4 4 3/8
[C2]* 8 8 0/16 - - n.d. - - n.d. 6 5 0/11
[MSA6]* 8 8 15/16 - - n.d. - - n.d. 6 5 5/11
[MSA13]* 8 7 13/15 - - n.d. - - n.d. 7 8 2/15

F, number of female mice inoculated; M, number of male mice inoculated; n, number of affected mice; n0, number of inoculated mice; n.d., not determined.

*

[Samples] indicate human patient samples passaged either in TgM83+/− mice and inoculated in Tg(SNCA*A53T+/+) animals or passaged in Tg(SNCA*A53T+/+) mice prior to inoculating in TgM83+/− animals.

Fig. 1. MSA prions retain biological and biochemical properties after passaging in Tg(SNCA*A53T+/+) mice.

Fig. 1.

(a) Kaplan–Meier plot of disease onset in TgM83+/− (dotted lines) and Tg(SNCA*A53T+/+)Nbm mice (solid lines) inoculated with either control sample C2 or MSA patient samples MSA6 and MSA13. TgM83+/− mice inoculated with MSA6 and MSA13 developed neurological disease (incubation times in Online Resource, Table S2; data previously reported in [31]). Tg(SNCA*A53T+/+)Nbm did not develop overt motor signs by 330 dpi. (b–d) Brain homogenate from Tg(SNCA*A53T+/+)Nbm mice that propagated MSA prions, as well as mice inoculated with control sample C2, were assessed for (b) biological and (c, d) biochemical properties of MSA prions. (b) Alpha-synuclein prions were isolated from brain homogenates via PTA precipitation and were incubated with α-syn–YFP cells for 4 d. Homogenates from MSA-inoculated mice showed poor infectivity in cells expressing the E46K mutation alone or in combination with the A53T mutation, and MSA prions were not able to propagate well in cells truncated at residue 95. Homogenates from mice inoculated with sample C2 showed no infectivity. Cell lines express WT α-syn–YFP, single mutations [A30P (P), E46K (K), and A53T (T)], double mutations [A30P,A53T (PT) and E46K,A53T (KT)] or A53T truncated at residue 95 (1-95) or 97 (1-97). (c, d) Alpha-synuclein prions in MSA-inoculated mice were denatured using increasing concentrations of GnHCl. Representative blots using the EP1536Y primary antibody are shown in (c). No insoluble protein was detected in aged Tg(SNCA*A53T+/+)Nbm mice or animals inoculated with C2 homogenate.

Table 2.

MSA prion propagation in Tg(SNCA) mice.

Patient Sample Mean cell infection ± SD, fluorescence/cell (103 A.U.)*
Tg(SNCA+/+)Nbm Tg(SNCA*A30P+/+)Nbm Tg(SNCA*A53T+/+)Nbm
C2 2.4 ± 1.0 1.6 ± 0.3 1.8 ± 0.8
C3 2.4 ± 1.0 2.0 ± 0.9 2.1 ± 1.0
C14 1.4 ± 0.3 1.9 ± 0.5 3.3 ± 3.5
C16 2.4 ± 1.0 1.9 ± 0.5 2.0 ± 0.6
C17 1.3 ± 0.4 1.7 ± 0.5 2.1 ± 0.4
MSA6 1.9 ± 0.4 3.3 ± 1.8 78 ± 12
MSA13 2.3 ± 0.6 1.8 ± 0.4 39 ± 22
MSA16 1.8 ± 0.3 2.3 ± 1.1 29 ± 26
MSA17 1.6 ± 0.7 1.7 ± 0.5 13 ± 16
MSA18 1.3 ± 0.1 4.0 ± 6.2 9.2 ± 10
*

Measurements made from five images per well, n = 6 wells. PTA-precipitated samples were diluted in DPBS 1:10 before testing on α-syn140*A53T–YFP cells.

MSA prions propagate in Tg(SNCA*A53T+/+)Nbm mice with high fidelity

Previously, we built a panel of HEK cells expressing α-synuclein containing various mutations and truncations [41]. Using these cells, we showed that MSA prions are unable to replicate in the presence of the PD-causing E46K mutation. Additionally, truncating α-syn*A53T at amino acid residue 95 also blocked in vitro propagation of MSA prions. To confirm the misfolded conformation, or strain, of α-synuclein in MSA patients propagates with high fidelity in Tg(SNCA*A53T+/+)Nbm mice, we tested brain homogenates from mice positive for α-synuclein infection in each of the seven cell lines represented in our panel: WT (α-syn140–YFP), E46K (α-syn140*E46K–YFP), A53T (α-syn140*A53T–YFP), A30P and A53T (α-syn140*A30P,A53T–YFP), E46K and A53T (α-syn140*E46K,A53T–YFP), A53T truncated at residue 95 (α-syn95*A53T–YFP), and A53T truncated at residue 97 (α-syn97*A53T–YFP). Homogenates from mice inoculated with control sample C2 did not infect any of the α-syn–YFP cell lines (Fig. 1b). In contrast, homogenates from MSA-inoculated mice that were positive for α-synuclein prions did infect α-syn140*–YFP, α-syn140*A53T–YFP, α-syn140*A30P,A53T–YFP, and α-syn97*A53T–YFP cells, but did not infect the α-syn140*E46K–YFP, α-syn140*E46K,A53T–YFP, or the α-syn95*A53T–YFP cell lines. These data demonstrate that MSA prions retain their biological activity after passaging in Tg(SNCA*A53T+/+)Nbm mice.

We biochemically characterized α-synuclein prions isolated from Tg(SNCA*A53T+/+)Nbm mice using GnHCl denaturation. Previously, we reported that MSA prions are ~50% denatured by 1.5 M GnHCl and completely denatured by 3 M GnHCl [41]. Here, we also found that α-synuclein aggregates from MSA-inoculated Tg(SNCA*A53T+/+)Nbm mice are fully denatured by 3 M GnHCl and ~50% denatured in 1.5 M GnHCl (Figs. 1c, d). Notably, neither aged Tg(SNCA*A53T+/+)Nbm nor Tg(SNCA*A53T+/+)Nbm mice inoculated with control patient samples developed insoluble α-synuclein inclusions (Fig. 1c).

MSA-inoculated Tg(SNCA*A53T+/+)Nbm mice develop α-synuclein neuropathology in the limbic system

Fixed half-brains from inoculated Tg(SNCA)Nbm mice were also analyzed for the presence of phosphorylated α-synuclein aggregates via immunofluorescence. Eight micron sections were cut and stained with the EP1536Y primary antibody for α-synuclein phosphorylated at residue Ser129. Consistent with the previous results, neither Tg(SNCA +/+)Nbm nor Tg(SNCA*A30P+/+)Nbm mice inoculated with MSA developed α-synuclein neuropathology (Online Resource, Figs. S2a, b). However, fixed tissue from Tg(SNCA*A53T+/+)Nbm mice that were positive for MSA prions in the cell assay did develop α-synuclein aggregates in the hippocampus and fimbria (Hc), the piriform cortex and amygdala (Pir), and the parahippocampal cortex (PHC; Fig. 2a–h). This predominantly limbic pathology was absent in mice inoculated with control sample C2. Notably, the distribution of α-synuclein inclusions in Tg(SNCA*A53T+/+)Nbm mice is in stark contrast to the hindbrain neuropathology that develops in MSA-inoculated TgM83+/− mice (Fig. 2i–o). Whereas TgM83+/− mice developed inclusions in the thalamus (Fig. 2m) and brainstem (Fig. 2o), these regions were unaffected in Tg(SNCA*A53T+/+)Nbm mice (Figs. 2f & h, respectively). Moreover, the Hc (Fig. 2j), fimbria (Fig. 2k), Pir (Fig. 2l), and PHC (Fig. 2n) were also unaffected in the TgM83+/− mice. These findings suggest that the difference in neurological presentation between the two models (Fig. 1a) is likely due to differences in the brain regions affected by MSA inoculation.

Fig. 2. Tg(SNCA*A53T+/+)Nbm mice inoculated with MSA develop α-synuclein neuropathology.

Fig. 2.

(ah) Tg(SNCA*A53T+/+)Nbm mice received an intracranial inoculation of 1% brain homogenate from one control or five MSA patient samples. Mice were collected 330 dpi, and fixed half-brains were cut coronally into four sections to allow analysis of phosphorylated α-synuclein inclusions in the caudoputamen (Cd), hippocampus and fimbria (Hc), piriform cortex and amygdala (Pir), sensory cortex (SCtx), thalamus (Thal), hypothalamus (HTH), parahippocampal cortex (PHC), and pons. (a) Tg(SNCA*A53T+/+)Nbm mice positive for MSA prions developed phosphorylated α-synuclein aggregates in the limbic system, whereas control inoculated mice did not. Pathology was measured from one slide per animal; C2: n=7; MSA6: n=6; MSA13: n=5; MSA16: n=4; MSA17: n=2; MSA18: n=3. (bh) Representative images of α-synuclein pathology in Tg(SNCA*A53T+/+)Nbm mice. (b) Full section including Hc, Pir, SCtx, Thal, and HTH shown with white boxes indicating magnified regions in (cf). For comparison, (io) representative images of pathology in a terminal TgM83+/− mouse inoculated with MSA. (i) Full section reveals a distinct staining pattern from (b). Higher magnification images show Hc (c, j), fimbria (d, k), Pir (e, l), Thal (f, m), PHC (g, n), and brainstem (h, o). Phosphorylated α-synuclein in green; DAPI in blue. Scale bars in (b, i), 500 μm; all others, 50 μm. * = P < 0.05; ** = P < 0.01; *** = P < 0.001.

Tg(SNCA*A53T+/+)Nbm mice were also inoculated with brain homogenate from five PD patient samples. Mice collected 330 dpi showed no phosphorylated α-synuclein pathology (data from mice inoculated with sample PD6 shown in Online Resource, Fig. S2c). Similarly, no α-synuclein prions were detected in PD-inoculated mice using the α-syn140*A53T–YFP cell assay (Online Resource, Table S3).

Tg(SNCA*A53T+/+) mice inoculated with MSA develop neuronal and glial pathology

Bielschowsky’s silver staining on Tg(SNCA*A53T+/+)Nbm mice inoculated with MSA patient samples revealed silver-positive aggregates in the Hc and Pir (Fig. 3). Given this distinct distribution of inclusions, compared to the TgM83+/− mice, we were interested in determining which cell type(s) developed α-synuclein aggregates. We performed co-localization studies with the neuronal marker MAP2 in both Tg(SNCA*A53T+/+)Nbm and TgM83+/− mice inoculated with MSA patient samples (Fig. 4). As expected, TgM83+/− mice, which use the mouse prion protein promoter to drive transgene expression, developed inclusions that co-localized with MAP2 staining (Figs. 4d, e; arrows in bottom panel of Fig. 4e identify co-labeling). We also observed MAP2 co-localization with α-synuclein in the Hc and Pir of Tg(SNCA*A53T+/+)Nbm mice (Figs. 4a–c; arrows in bottom panels of Figs. 4b, c show co-labeling). However, a number of α-synuclein aggregates did not co-localize with MAP2 (arrowheads in the bottom panels of Figs. 4b, c), indicating α-synuclein inclusions form in multiple cell types in Tg(SNCA*A53T+/+)Nbm mice.

Fig. 3. Tg(SNCA*A53T+/+)Nbm mice develop silver-positive aggregates.

Fig. 3.

One slide from four MSA-inoculated Tg(SNCA*A53T+/+)Nbm mice with phosphorylated α-synuclein neuropathology were analyzed for the presence of silver-positive aggregates. Representative full section (a) shows magnified regions of the hippocampus (b) and piriform cortex (c) in black boxes, which contain labeled aggregates. Scale bar in (a), 500 μm. Scale bars in (b, c), 50 μm.

Fig. 4. Tg(SNCA*A53T) mice inoculated with MSA develop neuronal pathology.

Fig. 4.

Formalin-fixed half-brains from (ac) Tg(SNCA*A53T+/+)Nbm (n=21) and (de) TgM83+/− mice (n=2) inoculated with MSA brain homogenate were analyzed for co-localization of α-synuclein (green) with the neuronal marker MAP2 (purple). One slide per animal was assessed. (a, d) White boxes in low-magnification images identify regions of the hippocampus (i) and piriform cortex (ii) from Tg(SNCA*A53T+/+)Nbm mice and the brainstem (iii) from TgM83+/− mice. All regions are shown in higher magnification in the panels below as well as in Figs. 56. (b, c, e) Alpha-synuclein pathology (top panels) and MAP2 immunostaining (middle panels) co-localized in both mouse models (white, bottom panels). Arrows point to α-synuclein aggregates that co-localize with MAP2. Arrowheads identify α-synuclein aggregates that are not neuronal. Images shown from the (b) hippocampus, (c) piriform cortex, and (e) brainstem. DAPI in blue. Scale bars in (a, d), 500 μm. Scale bars in (b, c, e), 50 μm.

To determine which additional cells contained α-synuclein aggregates, we performed co-localization studies using antibodies specific for three glial cell types (Figs. 5 & 6). The neuronal α-synuclein aggregates in TgM83+/− mice were proximal to reactive astrocytes (GFAP; Fig. 5c) and microglia (Iba1; Fig. 5f), but as expected, none of the α-synuclein co-localized with either cell type in these mice (bottom panels). Alternatively, while some of the phosphorylated α-synuclein aggregates in the Tg(SNCA*A53T+/+)Nbm animals co-localized with microglia (indicated by arrows in Figs. 5d, e), the vast majority were found in astrocytes (indicated by arrows in Figs. 5a, b). Given that MSA is defined by the presence of GCIs in oligodendrocytes, we also assessed the co-localization of α-synuclein with Olig2, a marker for oligodendrocytes (Fig. 6). Alpha-synuclein inclusions in TgM83+/− mice did not co-localize with Olig2 immunostaining (Fig. 6c), but a small number of inclusions in the Hc and Pir of Tg(SNCA*A53T+/+)Nbm mice did (arrows in Figs. 5a, b).

Fig. 5. Tg(SNCA*A53T+/+)Nbm mice inoculated with MSA develop glial pathology.

Fig. 5.

Fixed tissue from both Tg(SNCA*A53T+/+)Nbm (n=21) and TgM83+/− mice (n=2) inoculated with MSA patient samples was analyzed for α-synuclein co-localization with (ac) astrocytes and (df) microglia. One slide per animal was assessed. (ac) Immunostaining for phosphorylated α-synuclein (green, top panels) and astrocytes (GFAP; purple, middle panels) shown from the (a) hippocampus and (b) piriform cortex of Tg(SNCA*A53T+/+)Nbm mice and the (c) brainstem of TgM83+/− mice, as identified in Figs 4a, d. Merged images (bottom panels) show co-localization of α-synuclein with GFAP in Tg(SNCA*A53T+/+)Nbm mice (arrows identify co-labeling in white), whereas no co-staining was seen in TgM83+/− mice. (df) The same sections were also analyzed for α-synuclein (top panels) co-localization with microglia (Iba1; red, middle panels). Some co-labeling (white, bottom panels) was seen in Tg(SNCA*A53T+/+)Nbm mice (white arrows), but no co-localization was observed in TgM83+/− mice. DAPI in blue. Scale bars, 50 μm.

Fig. 6. Tg(SNCA*A53T+/+)Nbm mice inoculated with MSA develop oligodendroglial inclusions.

Fig. 6.

Fixed tissue from both Tg(SNCA*A53T+/+)Nbm (n=10) and TgM83+/− mice (n=2) inoculated with MSA patient samples were analyzed for α-synuclein (green, top panels) co-localization with oligodendrocytes (Olig2; purple, middle panels). Some oligodendroglial inclusions (bottom panels show merged images) were seen in the (a) hippocampus and (b) piriform cortex of Tg(SNCA*A53T+/+)Nbm mice, but none were seen in the (c) brainstem of TgM83+/− mice. One slide per animal was assessed. Scale bars, 50 μm.

In addition to immunostaining results from the two Tg mouse models, we also analyzed fixed tissue from two MSA patient samples (MSA46 and MSA47; Online Resource, Fig. S3). In line with reports of neuronal α-synuclein inclusions in MSA patients [12, 25, 29], we observed some co-localization of α-synuclein with MAP2 (Online Resource, Fig. S3a). Looking at glial cells, we also identified a number of α-synuclein inclusions in astrocytes and microglia (Online Resource, Fig. S3b); however, as expected, α-synuclein predominantly aggregated in oligodendrocytes (Online Resource, Fig. S3c).

Intrigued by the finding that α-synuclein pathology in Tg(SNCA*A53T+/+)Nbm mice is frequently found in astrocytes, as well as a handful of oligodendrocytes, we analyzed both Tg mouse models and human samples for co-localization with NG2 immunostaining (Online Resource, Fig. S4). NG2 cells are polydendrocytes and are the fourth major glial cell type in the brain constituting 2–8% of cells in the adult CNS [26]. While NG2 cells were initially thought to only give rise to oligodendrocytes [3, 16, 32, 45, 48], it is now known they can also differentiate into astrocytes [10, 47, 48]. Recently, two groups reported co-localization of α-synuclein neuropathology with NG2 cells in MSA patient samples [15, 24]. Using immunofluorescence with an NG2-specific antibody to determine co-localization with α-synuclein, we found that neither Tg mouse model developed α-synuclein inclusions in NG2 cells (Online Resource, Fig. S4a). However, both MSA patient samples showed co-localization of phosphorylated α-synuclein with NG2 immunolabeling, though more co-staining was observed in patient MSA46 than MSA47, which may be due to differences in the clinical presentation between the two patients (MSA46 was diagnosed with the cerebellar phenotype while MSA47 was diagnosed with the parkinsonian phenotype; Online Resource, Fig. S4b). Confirming that the two MSA patient samples used for the immunofluorescence studies were consistent with the frozen tissue used to inoculate the Tg(SNCA*A53T+/+)Nbm mice, we isolated α-synuclein prions from fresh frozen tissue via PTA-precipitation from the same brain region from both samples. We then tested the α-synuclein prions for biological activity by incubating the samples with our panel of α-syn–YFP cell lines (Online Resource, Fig. S5). Consistent with our previous findings [41] and additional data reported here, the samples were able to infect the α-syn–YFP cells, with the exception of lines expressing the E46K mutation or truncation of the protein at residue 95.

MSA prions propagated in Tg(SNCA*A53T+/+)Nbm mice transmit disease to TgM83+/− animals

Observing that MSA prions retain their biological and biochemical properties upon inoculation, or passaging, in Tg(SNCA*A53T+/+)Nbm mice, we proposed that the brain homogenates from these animals would transmit disease to TgM83+/− mice. To address this hypothesis, we inoculated 10-week-old TgM83+/− mice with 1% brain homogenate from Tg(SNCA*A53T+/+)Nbm mice originally inoculated with control patient sample C2 or an MSA patient sample (MSA6 or MSA13). TgM83+/− mice inoculated with passaged C2 brain homogenate remained asymptomatic 450 dpi (Fig. 7a); however, mice inoculated with brain homogenate from either MSA6 or MSA13 patient samples passaged in Tg(SNCA*A53T+/+)Nbm mice developed neurological disease (MSA6: 119 ± 24 dpi; MSA13: 245 ± 85 dpi; P < 0.0001). Flash-frozen half-brains from terminal animals were homogenized, and the α-synuclein prion concentration was measured using the α-syn140*A53T–YFP cell assay (Fig. 7b). TgM83+/− mice inoculated with passaged C2 patient sample did not contain α-synuclein prions (2.9 ± 1.2 × 103 A.U.), but animals inoculated with the passaged MSA patient samples were positive for α-synuclein prions (MSA6: 71 ± 30 A.U.; MSA13: 54 ± 38 × 103 A.U.; P < 0.001). These α-synuclein prions retained biological characteristics of MSA prions. Testing for infectivity in the α-syn–YFP cell panel revealed α-synuclein isolated from TgM83+/− mice inoculated with passaged MSA patient samples infected α-syn140–YFP, α-syn140*A53T–YFP, α-syn140*A30P,A53T–YFP, and α-syn97*A53T–YFP cells, but it did not infect cells expressing the E46K mutation or α-synuclein truncated at residue 95 (Fig. 7c). These data argue MSA prions propagate with high fidelity between two Tg(SNCA*A53T) mouse models.

Fig. 7. MSA prions transmit disease to TgM83+/− mice after passaging in Tg(SNCA*A53T+/+)Nbm mice.

Fig. 7.

Brain homogenate from Tg(SNCA*A53T+/+)Nbm mice inoculated with either control (C2) or MSA (MSA6 or MSA13) patient samples was inoculated into TgM83+/− mice. (a) Mice inoculated with mouse-passaged MSA samples developed neurological disease, whereas mice inoculated with passaged C2 homogenate remained asymptomatic through 450 dpi. (b) Frozen half-brains from inoculated TgM83+/− mice were tested for MSA prions in the α-syn140*A53T–YFP cell assay (× 103 A.U.). Inoculation with passaged C2 homogenate did not induce α-synuclein prions (all samples below the dotted line), whereas mouse-passaged MSA samples induced α-synuclein prion formation. (c) Alpha-synuclein prions in TgM83+/− mice were analyzed for infectivity in additional α-syn–YFP cell lines. Samples infected cells expressing WT and mutant α-synuclein (A53T – T; A30P,A53T – PT; and A53T truncated at residue 97 – 1–97), but they did not infect cells expressing the E46K mutation alone (K) or in combination with the A53T mutation (KT). Additionally, they did not infect A53T-expressing cells truncated at residue 95 (1-95). (d, e) Fixed half-brains from the same mice were analyzed for phosphorylated α-synuclein neuropathology. Mice inoculated with the mouse-passaged C2 patient sample (n=11) did not develop α-synuclein aggregates, whereas inoculation of passaged MSA patient samples (MSA6: n=13; MSA13: n=9) induced widespread α-synuclein accumulation (green) and reactive astrocytes (GFAP; purple) and microglia (Iba1; red) in the hindbrain. Quantification of α-synuclein in the hippocampus (Hc), thalamus (Thal), hypothalamus (HTH), midbrain (Mid), and pons is shown in (d). Analysis was performed on one slide containing all brain regions from each animal. Representative images from the brainstem shown in (e). DAPI in blue. Scale bar, 50 μm. * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001.

We also assessed the formalin-fixed half-brains from the same animals. Consistent with our cell assay data, we found that serial passaging of the two MSA patient samples induced α-synuclein inclusions in the hindbrain of TgM83+/− mice (Figs. 7d, e). However, serial passaging with the control sample C2 did not.

MSA prions from symptomatic TgM83+/− mice propagate in Tg(SNCA*A53T+/+)Nbm animals

In previous studies, TgM83+/− mice inoculated with brain homogenate from MSA patient samples MSA6 and MSA13 induced neurological disease in the animals [42]. Brain homogenates from these mice, as well as from C2-inoculated animals terminated 450 dpi, were used to inoculate Tg(SNCA*A53T+/+)Nbm mice. These animals were collected 300 dpi, and flash-frozen half-brains were tested for the presence of MSA prions using the α-syn140*A53T–YFP cell assay (Fig. 8a). Mice inoculated with the passaged C2 patient sample did not develop α-synuclein prions (1.7 ± 0.5 × 103 A.U.). However, five mice inoculated with the mouse-passaged MSA6 patient sample (13 ± 13 × 103 A.U., P < 0.01) and two mice inoculated with the mouse-passaged MSA13 patient sample (4.5 ± 6.2 × 103 A.U., P = 0.57) did contain α-synuclein prions (samples above dotted line). The seven positive samples from serially passaging MSA and the homogenates from the control mice were further tested in the panel of α-syn–YFP cell lines (Fig. 8b). Consistent with the previous passaging data, homogenates from mice inoculated with mouse-passaged MSA patient samples infected the α-syn140–YFP, α-syn140*A53T–YFP, α-syn140*A30P,A53T–YFP, and α-syn97*A53T–YFP cells, but were unable to infect cells expressing the E46K mutation or α-synuclein truncated at residue 95. None of the samples from the mice inoculated with mouse-passaged C2 patient tissue infected the α-syn–YFP cells. These findings demonstrate the biological activity of MSA prions was retained during passaging in both TgM83+/− and Tg(SNCA*A53T+/+)Nbm animals.

Fig. 8. MSA prions passaged in TgM83+/− mice propagate in Tg(SNCA*A53T+/+)Nbm mice.

Fig. 8.

Brain homogenates from control (C2) or MSA patient samples (MSA6 or MSA13) first passaged in TgM83+/− mice were used to inoculate Tg(SNCA*A53T+/+)Nbm animals. Mice were collected 300 days post-inoculation. (a) Frozen half-brains were homogenized and assessed for the presence of α-synuclein prions using the α-syn140*A53T–YFP cell assay (× 103 A.U.). Passaged C2 homogenate did not induce α-synuclein prion formation, but seven mice inoculated with either passaged MSA6 or MSA13 patient samples propagated α-synuclein prions. (b) Alpha-synuclein prions in the Tg(SNCA*A53T+/+)Nbm mice were analyzed for infectivity in additional α-syn–YFP cell lines. Samples infected cells expressing wild-type (WT) and mutant α-synuclein (A53T – T; A30P,A53T – PT; and A53T truncated at residue 97 – 1-97), but they did not infect cells expressing the E46K mutation alone (K) or in combination with the A53T mutation (KT). Additionally, they did not infect A53T-expressing cells truncated at residue 95 (1-95). Homogenates from mice inoculated with passaged C2 patient sample did not infect any of the cells. (c,d) Fixed half-brains from the same mice were analyzed for phosphorylated α-synuclein neuropathology in the caudoputamen (Cd), hippocampus and fimbria (Hc), piriform cortex and amygdala (Pir), sensory cortex (SCtx), thalamus (Thal), hypothalamus (HTH), parahippocampal cortex (PHC), and pons. Mice inoculated with passaged C2 patient sample (n=11) did not develop α-synuclein aggregates, whereas inoculation of mouse-passaged MSA samples (MSA6: n=5; MSA13: n=2) induced α-synuclein accumulation in the Pir and PHC. Quantification performed using one slide containing all analyzed brain regions per animal (c). Representative images (d) show that the passaged MSA patient samples induced α-synuclein inclusions (green) that co-localized with astrocytes (GFAP; purple) and reactive microglia (Iba1; red). DAPI in blue. Scale bar, 50 μm. * = P < 0.01; ** = P < 0.01.

Finally, we analyzed the formalin-fixed half-brains from the seven mice positive for MSA prions and the control animals for α-synuclein aggregation via immunostaining (Figs. 8c, d). The control animals did not develop phosphorylated α-synuclein neuropathology, but the mice inoculated with the passaged MSA patient samples developed inclusions in the Pir and PHC. Notably, the hindbrain regions affected in TgM83+/− mice (Fig. 7d) were absent of pathology in the Tg(SNCA*A53T+/+)Nbm animals (Fig. 8c).

Discussion

Over the last five years, several studies have demonstrated that TgM83+/− mice inoculated with brain homogenate from MSA patients develop neurological disease ~120 dpi [31, 4043]. Alternatively, one study using Tg(SNCA+/+)Nbm mice found the animals remained asymptomatic for >12 months post-inoculation with MSA homogenates [31], whereas a second study in these animals found insoluble fractions from MSA patient samples induced α-synuclein inclusions within 9 months [1]. To investigate this difference, we inoculated Tg(SNCA+/+)Nbm mice, as well as animals expressing mutant α-synuclein [Tg(SNCA*A30P+/+)Nbm and Tg(SNCA*A53T+/+)Nbm], with brain homogenate from five MSA patient samples and five control samples. In the absence of neurological signs, mice were terminated either 400 [Tg(SNCA+/+)Nbm] or 330 dpi [Tg(SNCA*A30P+/+)Nbm and Tg(SNCA*A53T+/+)Nbm]. Using a cell-based assay to detect α-synuclein prions in brain homogenates from the mice [43], we found MSA prions had propagated in the Tg(SNCA*A53T+/+)Nbm mice, but not in the other two Tg(SNCA)Nbm lines. Mice containing MSA prions developed phosphorylated α-synuclein inclusions mainly in neurons and astrocytes throughout the limbic system. Additionally, the α-synuclein aggregates in these mice retained biological and biochemical characteristics of α-synuclein in MSA patient samples [41], indicating faithful propagation of MSA prions in vivo.

Intriguingly, SNCA transgene expression is lowest in Tg(SNCA*A53T+/+)Nbm mice, but it was the only line to propagate MSA prions. This finding suggests that the A53T mutation disfavors the native conformation of α-synuclein [27], stabilizing the misfolded conformation in MSA, or reducing the energy barrier between the two. Three groups have recently used cryo-electron microscopy (cryo-EM) to solve the structure of recombinant α-synuclein fibrils, and all three identified a pair of protofilaments forming a steric zipper involving the alanine at residue 53 [9, 19, 20]. In addition to this “rod polymorph,” a second paired protofilament (the “twister polymorph”) assigned the R group of residue 53 to the exposed surface of the protein where it does not appear to contribute to the protofilament structure [19]. Analysis of both conformations by Li et al. showed that the A53T mutation destabilizes the steric zipper in the rod polymorph, whereas the same mutation has little impact on the energy requirements for the twister polymorph. The ability of MSA patient samples to propagate in mice expressing SNCA*A53T argues that α-synuclein does not misfold into the rod polymorph in MSA patients. However, it is not clear from our data whether or not MSA arises from α-synuclein misfolding into the twister conformation or another, still undiscovered, conformation.

Transmission of MSA to a second Tg(SNCA*A53T) mouse model provides additional support for the hypothesis that MSA is caused by α-synuclein prions. In MSA-inoculated TgM83+/− mice, neurological disease is thought to arise from the neuropathological accumulation of α-synuclein prions in the hindbrain where they disrupt autonomic and motor function. Perplexingly, we detected α-synuclein prions in MSA-inoculated Tg(SNCA*A53T+/+)Nbm mice in the absence of gross neurological signs. This discrepancy was explained when immunostaining of phosphorylated α-synuclein inclusions in the animals revealed that the hindbrain is unaffected by MSA prions. Instead, α-synuclein aggregates were widespread in the hippocampus and fimbria, piriform cortex and amygdala, and parahippocampal cortex. Inclusions in these brain regions would not be expected to induce autonomic or motor deficits.

Transgene expression in TgM83+/− mice is driven by the prion protein promoter [5], resulting in neuronal SNCA*A53T expression and, consequentially, neuronal pathology in mice inoculated with MSA patient samples. Alternatively, the Tg(SNCA)Nbm lines were established using a P1 artificial chromosome, which contains the full human SNCA gene, its normal intron and exon structure, and 35 kb of upstream regulatory elements, meaning transgene expression is not limited to a single cell type or the CNS [17]. Previous studies using Tg(SNCA+/+)Nbm mice found MSA-induced α-synuclein aggregates were predominantly localized to neurons, with occasional inclusions seen in astrocytes or microglia [1]. This is similar to our observation that α-synuclein inclusions in MSA-inoculated Tg(SNCA*A53T+/+)Nbm mice primarily co-localize with neurons and astrocytes, with infrequent aggregates seen in microglia and oligodendrocytes.

The work reported here is the first demonstration that MSA prions can be transmitted between two Tg mouse models. Notably, the biological and biochemical characteristics of the α-synuclein prions that cause MSA were retained after primary passaging in both the TgM83+/− [41] and Tg(SNCA*A53T+/+)Nbm animals. Based on these observations, we proposed that the α-synuclein prions in MSA-inoculated Tg(SNCA*A53T+/+)Nbm mice would transmit neurological disease to TgM83+/− mice. We investigated this hypothesis using a crossover experiment in which two MSA patient samples and one control sample were first passaged in either TgM83+/− or Tg(SNCA*A53T+/+)Nbm mice (Fig. 9). Brain homogenates from those mice were subsequently used as inoculum in secondary passage experiments between the two Tg mouse models. Serial passaging with the control patient sample had no effect in either SNCA*A53T mouse line, indicating that the crossover inoculations alone were not responsible for inducing α-synuclein prion formation. Remarkably, we found that MSA prions first passaged in Tg(SNCA*A53T+/+)Nbm mice transmitted neurological disease to TgM83+/− animals, and we could quantify α-synuclein prions in brain homogenates from the symptomatic mice using the α-syn140*A53T–YFP cell assay. In parallel, we found that we could also detect α-synuclein prions in Tg(SNCA*A53T+/+)Nbm mice 300 days after inoculating them with MSA patient samples that had first been passaged in TgM83+/− mice. Despite the unequivocal neuropathological differences between TgM83+/− and Tg(SNCA*A53T+/+)Nbm mice after both primary and secondary passaging studies (both cell type(s) and brain regions affected), the α-synuclein prions transmitted between the two models remained unchanged from the initial MSA prions inoculated. Consistent with recent cryo-EM data, this finding argues that the conformation of the misfolded protein (rather than cellular milieu [30], mouse strain, or varied transgene expression) is responsible for determining the α-synuclein prion strain present in MSA patients. Altogether, these observations provide increasing evidence to support the hypothesis that MSA is a prion disease.

Fig. 9. Crossover studies show MSA prions can be passaged between two Tg(SNCA*A53T) mouse models.

Fig. 9.

Schematic showing the primary and secondary inoculations of MSA patient samples in TgM83+/− and Tg(SNCA*A53T+/+)Nbm mice. Patient samples MSA6 and MSA13 were first inoculated into either TgM83+/− or Tg(SNCA*A53T+/+)Nbm mice. TgM83+/− mice developed motor deficits, and their brains contained neuronal α-synuclein prions that accumulated throughout the hindbrain. Tg(SNCA*A53T+/+)Nbm mice did not exhibit motor deficits, but their brains did contain α-synuclein prions that formed inclusions in multiple cell types throughout the limbic system. Secondary passaging with these brain homogenates across the two Tg mouse models showed that TgM83+/− still developed motor deficits, α-synuclein prions, and neuronal hindbrain pathology, whereas Tg(SNCA*A53T+/+)Nbm mice remained asymptomatic, developed α-synuclein prions, and exhibited limbic pathology. Alpha-synuclein prions isolated from the Tg mouse models after the primary and secondary passages retained the same biological activity as the α-synuclein prions in MSA6 and MSA13 patient samples.

Supplementary Material

1

ACKNOWLEDGMENTS

We thank Robert Nussbaum for providing the Tg(SNCA)Nbm mice and the Hunters Point animal facility staff for breeding and caring for the animals used in this study. We also thank Eric Huang for his helpful discussion of the manuscript, and Martin Ingelsson (Uppsala University) and Deborah Mash (Miami Brain Brank) for providing control tissue. This work was supported by grants from the National Institutes of Health (AG002132 and AG031220), as well as by support from the Glenn Foundation and Daiichi Sankyo. The Sydney Brain Bank is supported by Neuroscience Research Australia and the University of New South Wales. Glenda M. Halliday is a National Health and Medical Research Council of Australia Senior Principal Research Fellow (1079679). The Parkinson’s UK Brain Bank at Imperial College London is funded by Parkinson’s UK, a charity registered in England and Wales (948776) and in Scotland (SC037554). The Massachusetts Alzheimer’s Disease Research Center is supported by the National Institutes of Health (AG005134), and the Neurodegenerative Disease Brain Bank at the University of California, San Francisco, receives funding support from NIH grants P01AG019724 and P50AG023501, the Consortium for Frontotemporal Dementia Research, and the Tau Consortium.

Footnotes

Conflict of interest

The Institute for Neurodegenerative Diseases has a research collaboration with Daiichi Sankyo (Tokyo, Japan). S.B.P. is the chair of the Scientific Advisory Board of Alzheon, Inc., and a member of the Scientific Advisory Board of ViewPoint Therapeutics, neither of which has contributed financial or any other support to these studies.

COMPLIANCE WITH ETHICAL STANDARDS

Ethical approval

Animals were maintained in an AAALAC-accredited facility in compliance with the Guide for the Care and Use of Laboratory Animals. All procedures used in this study were approved by the University of California, San Francisco, Institutional Animal Care and Use Committee.

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