Figure 4. CS-evoked freezing in rats utilized for Fos analyses.
(A) Behavioral schematic. (B) Freezing during conditioning and retrieval testing. For conditioning, the left panel depicts mean percentage freezing during the 5 min baseline (BL) and across each conditioning block (each 136 s block is comprised of two trials; conditioning trials consist of freezing during the 10 s CS followed by the 58 s interstimulus interval). For retrieval testing, the center panel shows mean percentage freezing at the 5 min baseline (BL) and across each test block (each 140 s block is comprised of two trials; trials consist of freezing during the 10 s CS followed by the 60 s interval). The right panel shows mean percentage freezing after BL (corresponding to 840 s of behavior). Animals were sacrificed for Fos analyses 90 min after trial 1. All data are represented as means ± s.e.m [FW (n = 8); BW (n = 14); NoCS(Test) (n = 17); NoTest (n = 8)]; * = p < 0.05.