Draft Genome Sequence of Lactobacillus plantarum CRL681, Isolated from Argentinean Artisanal Fermented Sausages

Lactobacillus plantarum CRL681 was isolated from Argentinean artisanal fermented sausages. Here, the draft genome sequence of the CRL681 strain is described.

ABSTRACT

Lactobacillus plantarum CRL681 was isolated from Argentinean artisanal fermented sausages. Here, the draft genome sequence of the CRL681 strain is described. The reads were assembled into contigs with a total estimated size of 3,370,224 bp. A total of 3,300 open reading frames (ORFs) were predicted, including 3,126 protein-coding sequences. The draft genome sequence of L. plantarum CRL681 will be useful for understanding the organism’s metabolic activities and for biotechnological applications.

ANNOUNCEMENT

Lactobacillus plantarum CRL681, a strain originally isolated from an artisanal Argentinean fermented sausage, has an efficient acidogenic activity that guarantees safety and texture development during ripening of fermented sausages (1–7). The CRL681 strain possesses different aminopeptidases, and it has been demonstrated to have the ability to contribute to meat protein degradation by promoting the activity of muscle proteolytic enzymes (2, 3, 5, 8). Detailed peptidomic studies confirmed its peptidogenic ability and its capacity to increase free amino acid contents when inoculated into raw meat or fermented-meat models (6, 8, 9). On the other hand, L. plantarum CRL681 is capable of degrading biogenic amines in vitro and lacks the ability to produce them from amino acids, indicating the absence of amino oxidase and amino decarboxylase activities, respectively (4). In addition, the CRL681 strain has remarkable bioprotective potential due to the high inhibitory activity toward Escherichia coli O157:H7 (10).

L. plantarum CRL681 was grown for 12 h at 30°C (final log phase) in Man-Rogosa-Sharpe (MRS) agar (Oxoid, Cambridge, UK). A single colony was picked for DNA isolation. Library preparation was performed using a Nextera XT DNA library prep kit following the manufacturer’s protocol. Briefly, 1 ng of DNA (5 μl of the sample normalized to 0.2 ng/μl) was submitted to enzymatic fragmentation by transposons and end labeling, followed by adapter ligation, amplification, and purification of DNA fragments. The L. plantarum CRL681 genomic DNA was sequenced with the 2 × 150-bp paired-end read length sequencing protocol of the Illumina MiSeq platform. The quality of the reads was controlled using FastQC (11), and the generated sequencing reads were filtered to remove low-quality reads using Prinseq (12) with the following parameters: Min_len, 150; Trim_left, 15; Trim_right, 10; and Min_qual_mean, 25. SPAdes v3.11.1 (13) was used for de novo assembly with an N₅₀ value of 449,362 bp. The sequencing protocol generated 191× mean coverage of the genome. The CRL681 draft genome sequence contains 28 contigs with an average GC content of 44.3% and a total estimated size of 3,370,224 bp.

The Rapid Annotations using Subsystems Technology (RAST) server was used for functional annotation of predicted genes (14). A total of 3,300 open reading frames (ORFs) were predicted, including 3,126 protein-coding sequences, 61 tRNAs, 17 rRNAs, and 4 noncoding RNAs (ncRNAs). No clustered regularly interspaced short palindromic repeats (CRISPRs) were found in the genome by using CRISPRFinder (15). Default parameters were used in all of the bioinformatic analyses.

Fifteen putative peptidases were detected when the genome of L. plantarum CRL681 was analyzed by RAST (12) and submitted to the online BLAST search tool on the MEROPS peptidase database (16), demonstrating the peptidolytic potential of this strain. Additionally, a choloylglycine hydrolase gene for bile hydrolysis was found in L. plantarum CRL681 that could be involved in its ability to survive in the gastrointestinal tract (17). Clusters of genes involved in the biosynthesis of folate and riboflavin were also found in the CRL681 genome.

The draft genome sequence of L. plantarum CRL681 will be useful for understanding its metabolic activities and biotechnological applications.

Data availability.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number QOSF00000000. The version described here is QOSF01000000. The SRA/DRA/ERA accession number is ERP111695. The BioSample and BioProject numbers are SAMN09649875 and PRJNA480792, respectively.