Figure 5.

In Vitro PDT Induced by 3 and 5

(A–D) MTT assays of HeLa (A and C) and MCF-7 (B and D) cancer cells incubated with 2, 3, 4, and 5. Cells were incubated with DPBS dispersion of 2, 3, 4, or 5 (100 μL, 0, 0.2, 0.5, 1.0, 2.0, 4.0 μM, BODIPY equiv.) for 30 min. Then the cells were exposed to green LED (40 mW/cm²) for 0 min (A and B) and 15 min (C and D), respectively. After additional 24-h incubation, the relative cell viabilities were detected by the standard MTT assay. Data were presented as mean ± SD (n = 5).

(E and F) Laser scanning confocal images of HeLa (E) or MCF-7 (F) cells co-stained with calcein-AM (green, live cells) and propidium iodide (red, dead cells) after being incubated with 3 or 5 (2.0 μM, BODIPY equiv.) with green LED irradiation (40 mW/cm²) for 0, 5, 15 min, respectively. Scale bar, 500 μm.

(G and H) In vitro scratch assays. MCF-7 cell monolayer with scratches was incubated with 3 or 5 (500 μL, 0.5 μM, BODIPY equiv.) for 30 min. Then cells were exposed to green LED (40 mW/cm²) for 0 or 5 min, respectively. The cells that were not incubated with 3 and 5 were used as controls. Representative images (G) and scratch width ratio of 0 h and 24 h (H) are shown. Scale bar, 200 μm. Data were presented as mean ± SD (n = 3, **p < 0.01, *p < 0.05).