Fig. 3.

Localized expression and translation of Lck-regulated migration transcripts in hGC pseudopodia. a RNA from purified pseudopodia of hGCs from two patients (GSC1) and (GSC2) with glioblastomas was used to perform a qPCR array for migration-specific genes (SA Biosciences). Heat map expression analysis shows that RNA isolated from pseudopodia of hGCs contains various migration-specific transcripts including Lck, Paxillin, CrkII, and Rac1 (arrows). b Polysome profile of hGC pseudopodia following sucrose density gradient fractionation. The presence of 40S, 60S, 80S, and polyribosomes is noted on the profile. c Cycle threshold (Ct) values of Lck, Paxillin, CrkII, and Rac1 transcripts bound to polyribosomes in hGC pseudopodia. The Ct values were obtained from three independent experiments for each hGC sample. The graph shows the individual data points for each experiment and the bars represent the mean Ct values ± standard deviation. d Western blotting for the detection of p(Y394)-Lck, p(Y118)-Paxillin, p(Y221)-CrkII, and the corresponding total proteins as loading controls in protein lysates isolated from pseudopodia of two patients derived hGCs, shows the local presence of the phosphorylated proteins. The experiment was repeated three times for each hGC