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Mycoplasma contamination increased the gene expression and protein secretion of inflammatory factors. a There were strong expression of IL-1β, IL-6, TNF-α, Cox2 and iNOS in BV2-MC by semi-quantitative RT-PCR detection. b, c ELISA analysis showed that secretion of IL-6 and TNF-α increased significantly after mycoplasma contamination. (BV2-N: normal BV2 cell, BV2-MC: mycoplasma-contaminated BV2 cell, BV2-MR: mycoplasma-removed BV2-MC cell). (*P < 0.05)
