Figure 1.

Regulation of interferon-stimulated gene (ISG) transcript levels following infection with intact or ultraviolet (UV)-irradiated human cytomegalovirus (HCMV) in cells with an abrogated interferon (IFN) response. Primary human foreskin fibroblasts (HFs), nPro expressing HFs (nPro/HFs) and V protein-expressing HFs (V/HFs) were infected in parallel with HCMV or UV-HCMV at an MOI of 3. 6 h.p.i. RNA was extracted, converted to cDNA and the relative levels of various ISG transcripts (normalised to the housekeeping gene GAPDH) were calculated. Individual bars represent the average fold change in transcript level compared to the mock infection for each cell type (set to 1). Error bars indicate the SEM and statistical significance was determined using a Student’s two-tailed t-test, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.