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. 2019 Mar 7;8(3):223. doi: 10.3390/cells8030223

Figure 8.

Figure 8

CTGF is required for migration and invasion induced by FGFR1 paracrine activation in MDA-MB-231 cells. Transwell assays were used to assess cell migration (a) and invasion (b) in MDA-MB-231 cells transfected for 24 h with control shRNA or shCTGF and then cultured for 8 h in CM from CAFs treated for 18 h with vehicle [CM/CAFs (+vehicle)], 10 nM E2 [CM/CAFs (+E2)] or 100 nM G-1 [CM/CAFs (+G-1)]. Cells were counted in at least 10 random fields at 10× magnification, in three independent experiments performed in triplicate. Scale bar = 200 μm, (**) indicates p < 0.01.