Table 1.
Antibodies list.
| Antibody | Number | Company | Dilution |
|---|---|---|---|
| α-SMA | A5228 | Sigma, USA | IHC 1:80 |
| β-actin | MAB1501 | Millipore, Germany | WB 1:1000 |
| β-catenin | ab6302 | Abcam, UK | WB 1:500 |
| BSEP | ab217532 | Abcam, UK | IHC 1:80 |
| c-Myc | ab32 | Abcam, UK | WB 1:500 |
| CCR7 | ab1657 | Abcam, UK | WB 1:500; IHC 1:80 |
| CD11b | ab133357 | Abcam, UK | IHC 1:80 |
| CD11c | ab11029 | Abcam, UK | IHC 1:80 |
| CD11c | ab52632 | Abcam, UK | WB 1:500 |
| CD163 | ab182422 | Abcam, UK | WB 1:500; IHC 1:80 |
| CD206 | ab64693 | Abcam, UK | WB 1:500; IHC 1:80 |
| CD34 | ab8158 | Abcam, UK | WB 1:500; IHC 1:80 |
| CD36 | SC-70644 | Santz cruz, USA | WB 1:500; IHC 1:80 |
| CD36, Alexa Fluor 647 | 102609 | BioLegend, USA | IF 1:100 |
| CD90 | ab3105 | Abcam, UK | WB 1:500; IHC 1:80 |
| CYP7A1 | SC-518007 | Santz cruz, USA | WB 1:500; IHC 1:80 |
| F4/80 | 123102 | BioLegend, CA | IHC 1:80 |
| FGFR4 | ab44971 | Abcam, UK | IHC 1:80 |
| FXR | SC-13063 | Santz cruz, USA | WB 1:500; IHC 1:80; IF 1:80 |
| G6Pase | SC-25840 | Santz cruz, USA | WB 1:500 |
| HIF-1α | ab1 | Abcam, UK | IHC 1:60 |
| Histone | SC-56616 | Santz cruz, USA | WB 1:1000 |
| MRP3 | ab3375 | Abcam, UK | IHC 1:80 |
| Neutrophil | ab2557 | Abcam, UK | IHC 1:80 |
| NF-κB | ab16502 | Abcam, UK | WB 1:500; IHC 1:80; IF 1:80 |
| Notch1 | ab52627 | Abcam, UK | WB 1:500 |
| NICD | ab8925 | Abcam, UK | WB 1:500 |
| NTCP | ab131084 | Abcam, UK | IHC 1:80 |
| OATP1 | ab203036 | Abcam, USA | IHC 1:80 |
| OSTβ | orb1964 | Biorbyt, USA | IHC 1:80 |
| PEPCK | SC-377027 | Santz cruz, USA | WB 1:500 |
| PGC-1α | ab54481 | Abcam, UK | WB 1:500; IHC 1:80 |
| SIRT1 | ab110304 | Abcam, UK | WB 1:500; IHC 1:80 |
| SREBP-1c | SC-366 | Santz cruz, USA | WB 1:500; IHC 1:100; IF 1:80 |
| p-STAT3 | 05-485 | Millipore, Germany | WB 1:500 |
| STAT3 | 06-596 | Millipore, Germany | WB 1:500 |
| TGFβ RII | SC-17791 | Santz cruz, USA | WB 1:500; IHC 1:100 |
| TGR5 | ab72608 | Abcam, UK | IHC 1:80 |
| TLR4 | ab13556 | Abcam, UK | IHC 1:80 |
| Tmem26 | ab186640 | Abcam, UK | WB 1:500; IHC 1:80 |
| UCP1 | ab10983 | Abcam, UK | WB 1:500; IHC 1:80 |
| VCAM | SC-8304 | Santz cruz, USA | IHC 1:80 |
| VEGF | SC-80434 | Santz cruz, USA | IHC 1:80 |
| VEGF | ab69479 | Abcam, UK | WB 1:500 |
| VEGFRI | 1303-1 | Epitomics, USA | WB 1:500; IHC 1:80 |
| DAPI | 62248 | Thermo Fisher Scientific, USA | IF 1:10000 |
| Mouse Secondary Antibody | G-21040 | Thermo Fisher Scientific, USA | WB 1:3000; IHC 1:100 |
| Rabbit Secondary Antibody | G-21234 | Thermo Fisher Scientific, USA | WB 1:3000; IHC 1:100 |
| Goat Secondary Antibody | 31402 | Thermo Fisher Scientific, USA | WB 1:3000; IHC 1:100 |
| Rat Secondary Antibody | 31470 | Thermo Fisher Scientific, USA | WB 1:3000; IHC 1:100 |
| Mouse Secondary Antibody, Alexa Fluor 488 | A-11029 | Thermo Fisher Scientific, USA | IF 1:100 |
| Rabbit Secondary Antibody, Alexa Fluor 488 | A11034 | Thermo Fisher Scientific, USA | IF 1:100 |
| Rabbit Secondary Antibody, Alexa Fluor Plus 647 | A32795 | Thermo Fisher Scientific, USA | IF 1:100 |
α-SMA, α-smooth muscle actin; BSEP, bile salt export pump; CCR7, C-C chemokine receptor type 7; CD11b, cluster of differentiation 11b; CYP7A1, cholesterol 7 alpha-hydroxylase; FGFR4, fibroblast growth factor receptor 4; FXR, farnesoid X receptor; G6Pase, glucose 6-phosphatase; HIF-1α, hypoxia-inducible factor-1α; MRP3, multidrug resistance-associated protein 3; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; Notch1, neurogenic locus notch homolog protein 1, NICD, Notch intracellular domain; NTCP, Na+-taurocholate cotransporting polypeptide; OATP1, organic anion-transporting polypeptide 1; OSTβ, organic solute transporter β; PEPCK, phosphoenolpyruvate carboxykinase; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator-1α; SIRT1, NAD-dependent deacetylase sirtuin 1; SREBP-1c, sterol regulatory element-binding protein-1c; STAT3, signal transducer and activator of transcription 3; TGFβ RII, transforming growth factor beta receptor II; TGR5, G-protein-coupled bile acid receptor 1, TLR4, Toll-like receptors 4; Tmem26, transmembrane protein 26; UCP1, uncoupling protein 1; VCAM, vascular cell adhesion molecule; VEGF, vascular endothelial growth factor; VEGFRI, VEGF receptor I; DAPI, 4’,6-diamidino-2-phenylindole; IHC, immunohistochemistry; IF: immunofluorescence; WB, Western blot.