Table 2.
Oligonucleotide sequences for-qPCR.
| Gene | Forward | Reverse |
|---|---|---|
| SREBP-1c | 5′ actgtcttggttgttgatgagctggagcat 3′ | 5′ atcggcgcggaagctgtcggggtagcgtc 3′ |
| FAS | 5′ tgtcattggcctcctcaaaaagggcgtcca 3′ | 5′ tcaccactgtgggctctgcagagaagcgag 3′ |
| SCD-1 | 5′ ccggagaccccttagatcga 3′ | 5′ tagcctgtaaaagatttctgcaaacc 3′ |
| FATP | 5′ gcttcaacagccgtatcctc 3′ | 5′ tcttcttgttggtggcactg 3′ |
| CD36 | 5′ gcaaaacgactgcaggtcaac 3′ | 5′ tggtcccagtctcatttagcca 3′ |
| CPT-1 | 5′ ggacagagactgtgcgttcct 3′ | 5′ gcgatatccaacagtgcttga 3′ |
| CPT-2 | 5′ caaggccctggctgatgatgtg 3′ | 5′ agtctctgtccgcccctctcg 3′ |
| ACO | 5′ atgaatcccgatctgcgcaaggagc 3′ | 5′ aaaggcatgtaacccgtagcactcc 3′ |
| PPARα | 5′ cgtacggcaatggctttatc 3′ | 5′ aacggcttcctcaggttctt 3′ |
| PPARγ | 5′ cctcaaacttggcaatactc 3′ | 5′- agcaacaacataagcgtcat 3′ |
| Ppargc1b | 5′ cagccagtacagccccgatg 3′ | 5′ ggtgtgtcgccttcatccag 3′ |
| ATGL | 5′ aacaccagcatccagttcaa 3′ | 5′ ggttcagtaggccattcctc 3′ |
| HSL | 5′ agacaccagccaacggatac 3′ | 5′ catcaccctcgaagaagagca 3′ |
| DGAT | 5′ tcctgaattggtgtgtggtg 3′ | 5′ ggcgcttctcaatctgaaat 3′ |
| LCAD | 5′ tcaacagcagttacttgg 3′ | 5′ gacaatatctgagtggag 3′ |
| LPL | 5′ actcatctccgccatgcc 3′ | 5′ ccagctttctcctagcaagg 3′ |
| ACADM | 5′ ggggaggatgacggagcagc 3′ | 5′ cgggtactttaggatctggg 3′ |
| Notch1 | 5′ ccagcatggccagctctgg 3′ | 5′ catccagatctgtggccctgtt 3′ |
| Jag1 | 5′ gtccacggcacctgcaatg 3′ | 5′ caaggtttggcctcgcact 3′ |
| RBPJ | 5′ tggcactgttcaatcgcctt 3′ | 5′ aatcttgggagtgccatgcca 3′ |
| Dll1 | 5′ actccttcagcctgcctga 3′ | 5′ tatcggatgcactcatcgc 3′ |
| Dll3 | 5′ ctggtgtcttcgagctaca 3′ | 5′ acacgtgctagcaggttcc 3′ |
| Hes1 | 5′ aaagacggcctctgagcaca 3′ | 5′ tcatggcgttgatctgggtca 3′ |
| Hes5 | 5′ aagtaccgtggcggtggagatgc 3′ | 5′ cgctggaagtggtaaagcagctt 3′ |
| PEPCK | 5′ agcctgctccagctttga 3′ | 5′ ccctagcctgttctctgtgc 3′ |
| G6Pase | 5′ tgctgtgtctggtaggcaac 3′ | 5′ agaatcctgggtctccttgc 3′ |
| Pdk4 | 5′ gatcctaaccaccgccagcc 3′ | 5′ gcaaaggacgttctttcacag 3′ |
| Pdha1 | 5′ gccgagtgctggttgcttccc 3′ | 5′ gtctgcatcatcctgtagtacttgagcc 3′ |
| Gys1 | 5′ cggttgtcggacttgctagattgg 3′ | 5′ cataggtgaagtggtctggaaaggc 3′ |
| Gys2 | 5′ ccttggggtgtttccatcgtac 3′ | 5′ cggagaggtttgtagtcacactgg 3′ |
| Pik3r1 | 5′ cccactactgtagccaacaacagc 3′ | 5′ gagtgtaatcgccgtgcattttag 3′ |
| IL-6 | 5′-gtactccagaagaccagagg-3′ | 5′-tgctggtgacaaccacggcc-3′ |
| IL-10 | 5′ ctggctcagcactgctat 3′ | 5′ attcatggccttgtagacac 3′ |
| TNFα | 5′ ttgacctcagcgctgagttg 3′ | 5′ cctgtagcccacgtcgtagc 3′ |
| SOCS1 | 5′ gtggttgtggagggtgagat 3′ | 5′ cccagacacaagctgctaca 3′ |
| SOCS3 | 5′ taggaggcgcagccccaagg 3′ | 5′ gcggcgggaaacttgctgtg 3′ |
| STAT3 | 5′ cgacccaggtgctgccccgta 3′ | 5′ atgggggaggtagcacactccga 3′ |
| SIRT1 | 5′ gcaacagcatcttgcctgat 3′ | 5′ gtgctactggtctcactt 3′ |
| PGC-1α | 5′ gactcagtgtcaccaccgaaa-3′ | 5′ tgaacgagagcgcatcctt 3′ |
| TFAM | 5′ ggaatgtggagcgtgctaaaa 3′ | 5′-tgctggaaaaacacttcggaata 3′ |
| UCP1 | 5′ cctgcctctctcggaaacaa 3′ | 5′-tgtaggctgcccaatgaaca 3′ |
| Complex I (20kDa) | 5′ ccagctgcgcagagttcatc 3′ | 5′ gagagagcttggggaccacg 3′ |
| Complex II (Ip) | 5′ tctaccgctgccacaccatc 3′ | 5′ aagccaatgctcgcttctcc 3′ |
| Complex III (Core II) | 5′ ccattggaaatgcagaggca 3′ | 5′ ggctggtgacttcctttggc 3′ |
| Complex IV (Cox2) | 5′ tcatgagcagtcccctccct 3′ | 5′ gccatagaataaccctggtcgg 3′ |
| Complex V (F1α) | 5′ atctatgcgggtgtacgggg 3′ | 5′ agggactggtgctggctgat 3′ |
| p22phox | 5′ tggcctgattctcatcactgg 3′ | 5′ gggacaactccacagaaactc 3′ |
| p47phox | 5′ acatcacaggccccatcatccttc 3′ | 5′-atggattgtcctttgtgcc 3′ |
| NOX2 | 5′ actccttgggtcagcactgg 3′ | 5′ gttcctgtccagttgtcttcg 3′ |
| NOX4 | 5′ tgaactacagtgaagatttccttgaac 3′ | 5′ gacacccgtcagaccaggaa 3′ |
| Oatp1 | 5′ gtcttacgagtgtgctccagat 3′ | 5′ ggaatactgcctctgaagtggatt 3′ |
| Ntcp | 5′ caccatggagttcagcaaga 3′ | 5′ agcactgaggggcatgatac 3′ |
| Mrp2 | 5′ gcttcccatggtgatctctt 3′ | 5′ atcatcgcttcccaggtact 3′ |
| Mrp4 | 5′ ttagatgggcctctggttct ’ | 5′ gcccacaattccaaccttt 3′ |
| Sult2a1 | 5′ ggaaggaccacgactcataac 3′ | 5′ gattcttcacaaggtttgtgttacc 3′ |
| ABCA1 | 5′ tggacatcctgaagccag 3′ | 5′ ttcttcccacatgccct 3′ |
| ABCG1 | 5′ gctgggaagtccacactc 3′ | 5′ gatacggcacgagattgg 3′ |
| HMG Co S | 5′ tatgatggtgtagatgctgggaagtatacc 3′ | 5′ taagttcttctgtgcttttcatccac 3′ |
| HMG Co R | 5′ gggacggtgacacttaccatctgtatgatg 3′ | 5′ atcatcttggagagataaaactgcca 3′ |
| Tmem26 | 5′ accctgtcatcccacagag 3′ | 5′ tgtttggtggagtcctaaggtc 3′ |
| CD137 | 5′ cgtgcagaactcctgtgataac 3′ | 5′ gtccacctatgctggagaagg 3′ |
| Prdm16 | 5′ cagcacggtgaagccattc | 5′ gcgtgcatccgcttgtg 3′ |
| GAPDH | 5′ tcaccaccatggagaaggc 3′ | 5′ gctaagcagttggtggtgca 3′ |
SREBP-1c, sterol regulatory element-binding protein 1c; FAS, fatty acid synthase, SCD-1, stearoyl-CoA desaturase-1, FATP, fatty acid transport protein; CD36, cluster of differentiation 36; CPT-1, carnitine palmitoyltransferase 1; ACO, acyl-CoA oxidase; PPAR, peroxisome proliferator-activated receptor; ATGL, adipose triglyceride lipase; HSL, hormone-sensitive lipase; DGAT, diacylglycerol acyltransferases; LCAD, Long-chain acyl-CoA dehydrogenase; LPL, lipoprotein lipase; ACADM, medium-chain acyl-CoA dehydrogenase (MCAD); Jag1, Jagged1; RBPJ, recombining binding protein suppressor of hairless; Dll, Delta-like protein; Hes, hairy and enhancer of split; PEPCK, phosphoenolpyruvate carboxykinase; G6Pase, glucose 6-phosphatase; Pdk4, pyruvate dehydrogenase lipoamide kinase isozyme 4; Pdha1, pyruvate dehydrogenase alpha 1; Gys, glycogen synthase; Pik3r1, phosphatidylinositol 3-kinase regulatory subunit alpha; IL-6, interleukin-6; TNFα, tumor necrosis factor α; SOCS1, suppressor of cytokine signaling 1; STAT3, signal transducer and activator of transcription 3; SIRT1, NAD-dependent deacetylase sirtuin-1; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; TFAM, mitochondrial transcription factor A; UCP1, uncoupling protein 1; NOX1, NADPH oxidase 1; Oatp, organic anion-transporting polypeptide; Ntcp, Na+-taurocholate cotransporting polypeptide; Mrp2, multidrug resistance-associated protein 2; Sult2a1, sulfotransferase family 2A member 1; ABCA1, ATP-binding cassette transporter; ABCG1, ATP-binding cassette sub-family G member 1; HMG Co S, HMG-CoA Synthase; HMG Co R; HMG-CoA Reductase; Tmem26, transmembrane protein 26; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.