Adv. Sci. 2019, 6, 1802104
In the original published article, the axis in Figure 2G was marked as a logarithmic scale, even though it should be a linear scale. The corrected Figure 2 is presented below. The authors apologize for any confusion this may have caused.
Figure 2. Mesensphere‐derived MSCs exhibit exceptional morphorheological properties. A,B) Representative confocal microscopy maximal projections (Z = 15 μm) of mesensphere cytoskeletal structures and plastic‐adherent MSC cytoskeletal structures via staining of filamentous actin (F‐actin, green) and nuclei (blue). Scale bars: upper panels, 65 μm; lower panels, 15 μm. C) Schematic presentation of atomic force microscopy (AFM) measurement of cell stiffness by indentation at mesenspheres and plastic‐adherent MSCs. The mechanical properties of the cells were quantified using the apparent Young's modulus calculated from the obtained force–indentation curves. D) Histogram bars represent mean ± s.e.m. of two independent atomic force microscopy measurements including nine technical repeats. Statistical significance was determined using an unpaired two‐tailed t‐test. E) In real‐time deformability cytometry (RT‐DC), mesensphere MSCs and plastic‐adherent MSCs were flowed through a 30 μm × 30 μm microfluidic constriction and deformed without contact by shear stress and pressure gradients. F–H) Cellular morphorheological properties of mesensphere MSCs and plastic‐adherent MSCs were quantified as cell deformation, apparent Young's modulus, and cell size (cross‐sectional area; μm2) using automated image analysis. Histogram bars represent mean ± s.d. of four independent experiments. Statistical significance was determined using a 1D linear mixed model and a likelihood ratio test.