Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 16;10(2):e00446-19. doi: 10.1128/mBio.00446-19

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Crown copyright 2019.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 5 — tcdB receptor binding domain (RBD) diversity. (A) Organization of the four functional domains of the 2,366-amino-acid TcdB protein. The 597-bp variable region within the C-terminus receptor binding domain (RBD) is indicated (horizontal red bar). The figure was adapted from Dingle et al. (21). (B) Neighbor-joining phylogeny for 21 currently described tcdB RBD alleles. Tips are colored according to the MLST clade (see the color key). Novel alleles identified in this study (open green circles) are clustered with known MLST clade 5 allele type 1 (reported to date in the RT078 and RT126 lineages). Each sequence is 198 amino acid residues in length. Sequences were aligned using MUSCLE, and the tree was generated in MEGA6 with evolutionary distances calculated using the Tajima-Nei model. The scale bar shows the number of amino acid substitutions per site. The tree is midpoint rooted and supported by 500 bootstrap replicates. (C) Summary of nucleotide and amino acid changes in novel tcdB RBD variants.