Transcriptome data analyses of prostatic hyperplasia in Esr2 knockout rats

Abstract

Estrogen signaling plays an important role in the pathophysiology of prostatic hyperplasia. While signaling through estrogen receptor alpha (ESR1) increases proliferation of stromal cells, estrogen receptor beta (ESR2) plays an anti-proliferative and differentiating role in glandular epithelium. Disruption of ESR2 signaling resulted in prostatic glandular hyperplasia in the rat. To identify the ESR2-target genes, and the molecular mechanisms involved, we performed RNA-seq analyses in prostate glands of Esr2 knockout (Esr2−/−) and age matched wildtype rats. The raw data were analyzed using CLC genomics workbench. High quality RNA-seq reads were aligned to the Rattus norvegicus genome. Differentially expressed genes were identified based on an absolute fold change of 2 with pValue ≤0.05. Of the total 32,623 genes detected, 824 were differentially expressed in Esr2−/− prostate glands, 550 downregulated and 274 upregulated. Pathway analyses identified altered expression of genes involved in epithelial proliferation and benign tumor formation.

Specifications table

Subject area	Biology, Endocrinology
More specific subject area	Reproductive biology, Estrogen signaling
Type of data	RNA-seq data table in Excel format and figures
How data was acquired	Illumina HiSeq 4000 platform, Ingenuity pathway analysis
Data format	Normalized, filtered and analyzed data; Bioinformatics prediction
Experimental factors	ESR2-target genes in prostate that maintain normal growth and differentiation of glandular epithelium
Experimental features	Prostate tissue collected from 18-20-week-old wildtype, Esr2 knockout rats. Total RNA was isolated and cDNA libraries were prepared for RNA-sequencing. The raw reads of RNA-seq was analyzed by CLC Genomics Workbench. Differentially expressed genes (absolute fold changes of 2, p ≤ 0.05) were subjected to Ingenuity Pathway Analysis. Pathway analysis showed that the differentially expressed genes are involved in proliferation of epithelial cells, and development of the benign tumors.
Data source location	A basic science laboratory at the University of Kansas Medical Center, Kansas City, KS, USA
Data accessibility	Raw data of RNA Seq analysis are available on Sequence Read Archive (SRA) database and connected to BioProject PRJNA513841.

Value of the data •The data show transcriptome profile of Esr2−/− rat ventral prostate. •Differentially expressed genes in Esr2−/− prostate glands are involved in epithelial cell proliferation, and development of benign tumors. •Our data indicate that ESR2 is essential for maintaining normal growth of prostatic glandular epithelium.

1. Data

This article provides RNA-Seq data of wildtype and Esr2−/− rat ventral prostate. The list of differentially expressed genes (Absolute fold change ≥2, pValue ≤0.05) in Esr2−/− vs wildtype rats were shown in Table 1. Ingenuity pathway analysis of differentially expressed genes revealed that these genes were involved in proliferation of epithelial cell lines (Fig. 1). However, these genes also involved in formation of ‘Glandular intraepithelial neoplasm', Prostatic intraepithelial tumor' and Benign tumor (Fig. 2) in Esr2−/− rats.

Table 1.

Differentially expressed genes in prostate glands of Esr2 knockout rats.

Sl. No	Gene Symbol	Chrom No	Region	Max group mean	Log₂ fold change	Fold change	p-value	FDR p-value	Bonferroni
1	A2m	4	154309426..154359137	0.70	−1.11	−2.15	0.00	0.009	1.000
2	A2ml1	4	complement(161866078..161907767)	0.04	−3.46	−11.03	0.01	0.210	1.000
3	AABR07000658.1	1	complement(21145360..21146846)	0.95	1.09	2.12	0.00	0.080	1.000
4	AABR07001064.1	1	complement(36954496..36957300)	0.69	1.15	2.22	0.00	0.002	1.000
5	AABR07001068.1	1	37059412..37162589	2.41	1.20	2.30	0.00	0.020	1.000
6	AABR07001634.1	1	complement(54439977..54441579)	0.36	−1.46	−2.75	0.01	0.190	1.000
7	AABR07001807.1	1	complement(58339947..58345450)	0.14	−2.27	−4.84	0.00	0.020	1.000
8	AABR07001905.1	1	61786900..61818159	0.23	−1.52	−2.87	0.04	0.460	1.000
9	AABR07001926.2	1	complement(62207548..62223307)	0.65	−1.28	−2.44	0.02	0.300	1.000
10	AABR07002010.1	1	complement(64816762..64830953)	0.09	−2.75	−6.71	0.01	0.190	1.000
11	AABR07002247.1	1	69681569..69692765	0.33	1.55	2.93	0.00	0.100	1.000
12	AABR07002677.2	1	80372505..80382703	0.34	−1.48	−2.79	0.03	0.330	1.000
13	AABR07002784.2	1	complement(84953311..84959283)	1.12	1.14	2.20	0.03	0.390	1.000
14	AABR07003241.1	1	99864084..99865244	0.90	−1.10	−2.14	0.05	0.470	1.000
15	AABR07003241.2	1	complement(99866052..99870024)	0.39	−6.59	−96.29	0.04	0.410	1.000
16	AABR07005752.1	1	complement(195864391..195867147)	0.26	−1.00	−2.01	0.04	0.450	1.000
17	AABR07005779.4	1	complement(198162429..198168448)	0.16	1.52	2.88	0.01	0.170	1.000
18	AABR07006774.1	1	257724842..257729237	1.33	3.29	9.78	0.00	0.004	1.000
19	AABR07007121.1	1	282063929..282064741	0.48	−2.65	−6.26	0.00	0.030	1.000
20	AABR07007744.1	2	complement(26812444..26818679)	0.10	−1.85	−3.61	0.00	0.070	1.000
21	AABR07007874.1	2	complement(32297366..32297914)	2.68	−4.84	−28.71	0.00	0.000	0.001
22	AABR07007875.1	2	32310059..32310555	1.27	−3.09	−8.51	0.00	0.030	1.000
23	AABR07008462.1	2	59849941..59901109	0.59	−4.16	−17.93	0.00	0.000	0.000
24	AABR07009221.1	2	89254154..89259657	3.03	−1.38	−2.60	0.00	0.000	0.000
25	AABR07009787.1	2	complement(112358918..112368557)	1.68	−1.14	−2.21	0.00	0.001	0.650
26	AABR07010332.1	2	complement(132490612..132496152)	0.12	−3.70	−12.98	0.01	0.170	1.000
27	AABR07010468.1	2	complement(136903813..136992193)	0.69	−4.12	−17.34	0.00	0.007	1.000
28	AABR07010747.1	2	150013327..150023914	2.10	5.44	43.55	0.00	0.000	0.000
29	AABR07010985.1	2	complement(156302569..156353036)	0.04	−2.32	−4.99	0.03	0.340	1.000
30	AABR07010986.1	2	complement(156399129..156406032)	0.57	1.54	2.90	0.00	0.080	1.000
31	AABR07012065.1	2	complement(184692634..184694181)	0.73	−1.22	−2.32	0.00	0.070	1.000
32	AABR07012775.1	2	complement(208225406..208225888)	0.54	−1.94	−3.83	0.01	0.210	1.000
33	AABR07013425.1	2	complement(236822072..236823145)	0.44	−1.66	−3.16	0.01	0.110	1.000
34	AABR07015040.1	14	46286197..46287505	0.34	−3.74	−13.35	0.00	0.020	1.000
35	AABR07017874.1	15	32136645..32137490	1.19	1.52	2.87	0.00	0.020	1.000
36	AABR07020537.1	13	29839867..29845228	0.11	−1.15	−2.22	0.03	0.360	1.000
37	AABR07020999.1	13	52413241..52413723	0.45	1.69	3.22	0.02	0.300	1.000
38	AABR07024542.1	16	4439102..4446046	0.04	−2.89	−7.42	0.05	0.480	1.000
39	AABR07026143.1	16	complement(65369091..65369876)	4.63	1.16	2.24	0.00	0.020	1.000
40	AABR07026144.1	16	65430000..65522071	3.80	1.17	2.25	0.01	0.170	1.000
41	AABR07027128.1	17	complement(14942001..14949085)	0.08	−6.59	−96.21	0.04	0.410	1.000
42	AABR07027394.1	17	complement(27991458..27995913)	0.78	−1.09	−2.13	0.01	0.150	1.000
43	AABR07027450.1	17	complement(31779334..31780120)	0.83	1.94	3.85	0.00	0.090	1.000
44	AABR07027451.1	17	complement(31813411..31813716)	4.01	3.82	14.12	0.00	0.000	0.000
45	AABR07028349.1	17	66238947..66241440	0.28	1.42	2.67	0.02	0.240	1.000
46	AABR07028998.1	10	1771460..1783495	0.15	−2.53	−5.76	0.01	0.140	1.000
47	AABR07029803.1	10	complement(52023215..52028736)	0.03	−2.08	−4.24	0.05	0.480	1.000
48	AABR07029809.1	10	complement(52478050..52483325)	0.09	1.47	2.77	0.02	0.260	1.000
49	AABR07030563.1	10	93137598..93138165	0.53	−2.08	−4.23	0.02	0.240	1.000
50	AABR07030568.6	10	93266214..93282734	0.18	1.77	3.41	0.03	0.380	1.000
51	AABR07033318.1	11	complement(17281322..17286846)	0.11	−1.51	−2.85	0.01	0.130	1.000
52	AABR07034729.1	11	85263536..85430845	1.39	4.69	25.81	0.00	0.001	0.290
53	AABR07034833.1	12	complement(53872..54885)	7.52	1.20	2.30	0.00	0.001	0.280
54	AABR07036640.1	12	complement(51361186..51366445)	0.16	−3.68	−12.83	0.01	0.150	1.000
55	AABR07037356.1	X	complement(19384166..19386541)	0.46	−1.13	−2.19	0.00	0.090	1.000
56	AABR07037412.2	X	20216587..20225955	0.57	8.00	255.13	0.00	0.080	1.000
57	AABR07040288.1	X	96991658..96992125	1.29	−4.73	−26.61	0.00	0.020	1.000
58	AABR07040944.1	X	complement(115375018..115375492)	0.83	−1.49	−2.81	0.02	0.300	1.000
59	AABR07041096.1	X	complement(119194437..119200775)	1.92	−1.04	−2.06	0.00	0.000	0.000
60	AABR07042859.1	19	complement(12402521..12403635)	0.22	−3.27	−9.68	0.00	0.070	1.000
61	AABR07043772.1	19	39502153..39537050	0.30	−1.53	−2.89	0.01	0.230	1.000
62	AABR07044473.1	20	6945260..6946542	2.08	2.39	5.23	0.00	0.000	0.000
63	AABR07044570.1	20	10727513..10729072	1.02	1.91	3.75	0.00	0.000	0.004
64	AABR07045322.1	20	43142648..43143257	1.21	−2.03	−4.08	0.00	0.040	1.000
65	AABR07047750.2	5	45733655..45734034	0.62	−2.24	−4.74	0.01	0.160	1.000
66	AABR07047771.1	5	complement(46751661..46757181)	0.37	1.41	2.65	0.00	0.000	0.040
67	AABR07047844.1	5	complement(50371732..50373014)	2.16	2.51	5.70	0.00	0.000	0.000
68	AABR07049682.1	5	132773970..132800830	0.38	3.89	14.82	0.00	0.000	0.000
69	AABR07050393.1	5	complement(163713652..163715420)	1.24	1.11	2.15	0.00	0.080	1.000
70	AABR07050530.1	5	168474633..168475915	2.59	2.72	6.59	0.00	0.000	0.000
71	AABR07051532.1	3	complement(16440424..16441030)	0.50	−2.14	−4.40	0.02	0.310	1.000
72	AABR07051535.1	3	16590244..16590851	0.72	−2.69	−6.45	0.00	0.090	1.000
73	AABR07051548.1	3	complement(16749942..16753987)	0.44	5.86	57.94	0.04	0.460	1.000
74	AABR07051565.1	3	17180411..17181239	0.22	2.09	4.24	0.04	0.440	1.000
75	AABR07051583.1	3	17546566..17547291	9.04	4.23	18.78	0.00	0.000	0.000
76	AABR07051592.1	3	17648356..17649526	0.77	−3.48	−11.17	0.00	0.000	0.100
77	AABR07051626.1	3	complement(18244243..18244535)	3.15	8.33	322.19	0.00	0.060	1.000
78	AABR07051642.1	3	18601757..18602324	1.76	3.41	10.62	0.00	0.003	1.000
79	AABR07051658.1	3	18787606..18787893	4.36	8.88	470.90	0.00	0.030	1.000
80	AABR07051670.1	3	19045214..19045737	9.53	3.14	8.80	0.00	0.000	0.000
81	AABR07051673.1	3	19128400..19128929	0.50	1.62	3.08	0.04	0.430	1.000
82	AABR07051675.1	3	19141133..19141662	0.42	2.34	5.07	0.02	0.320	1.000
83	AABR07051678.1	3	19174027..19174559	0.48	2.07	4.21	0.02	0.290	1.000
84	AABR07051684.1	3	19274273..19274824	0.37	3.44	10.85	0.01	0.230	1.000
85	AABR07051689.1	3	19366370..19366657	21.10	7.62	196.50	0.00	0.000	0.000
86	AABR07051692.1	3	19441604..19441888	1.19	3.06	8.35	0.00	0.009	1.000
87	AABR07051716.2	3	20015136..20016146	0.39	−2.35	−5.09	0.02	0.260	1.000
88	AABR07051733.2	3	complement(20479688..20479999)	0.59	1.88	3.67	0.03	0.330	1.000
89	AABR07051741.1	3	20641664..20641978	0.64	1.92	3.78	0.03	0.380	1.000
90	AABR07052519.1	3	complement(59165353..59166356)	0.39	−1.67	−3.19	0.02	0.260	1.000
91	AABR07052664.1	3	complement(68304756..68310230)	0.09	−1.45	−2.73	0.02	0.300	1.000
92	AABR07053179.1	3	95614562..95620081	0.63	−1.52	−2.87	0.00	0.000	0.002
93	AABR07053707.1	3	121490812..121493714	0.05	−2.24	−4.73	0.04	0.450	1.000
94	AABR07053866.1	3	complement(131530330..131531612)	0.19	6.48	89.23	0.03	0.320	1.000
95	AABR07054716.1	3	167513759..167514438	1.31	−1.94	−3.83	0.01	0.200	1.000
96	AABR07055191.1	7	415625..454111	7.88	1.00	2.00	0.00	0.000	0.001
97	AABR07055492.1	7	complement(4126885..4127764)	0.30	−1.74	−3.34	0.02	0.230	1.000
98	AABR07058102.1	7	complement(100330324..100335512)	0.04	−2.99	−7.93	0.01	0.180	1.000
99	AABR07058410.1	7	complement(114944282..114945344)	0.11	−2.89	−7.41	0.04	0.460	1.000
100	AABR07058788.1	7	complement(137515388..137518589)	0.30	−1.72	−3.31	0.02	0.260	1.000
101	AABR07059159.1	4	complement(5721261..5773301)	0.22	−1.97	−3.93	0.01	0.130	1.000
102	AABR07060364.1	4	complement(69970575..69971689)	0.83	1.78	3.43	0.00	0.003	1.000
103	AABR07060886.1	4	98481520..98542683	4.32	2.63	6.20	0.00	0.000	0.000
104	AABR07060963.1	4	101882994..101883460	6.72	3.57	11.85	0.00	0.000	0.000
105	AABR07060979.1	4	complement(102123869..102124609)	0.37	−3.11	−8.61	0.03	0.350	1.000
106	AABR07060994.1	4	102351036..102351819	0.73	−6.54	−93.07	0.04	0.410	1.000
107	AABR07061001.1	4	102489916..102490375	16.67	3.97	15.62	0.00	0.000	0.000
108	AABR07061044.1	4	complement(103115238..103115522)	1.30	3.22	9.29	0.00	0.005	1.000
109	AABR07061048.1	4	complement(103144774..103145058)	1.48	2.62	6.16	0.00	0.010	1.000
110	AABR07061052.1	4	complement(103257811..103258134)	4.84	1.43	2.70	0.00	0.001	0.500
111	AABR07061134.1	4	106323089..106323738	3.09	2.77	6.81	0.00	0.000	0.000
112	AABR07064000.1	6	59757397..59757964	0.46	−4.04	−16.40	0.01	0.130	1.000
113	AABR07064349.1	6	76661416..76661824	0.66	1.24	2.35	0.04	0.430	1.000
114	AABR07064716.1	6	complement(91580777..91581262)	0.28	2.71	6.52	0.03	0.330	1.000
115	AABR07064755.1	6	93875374..93878199	0.15	−1.89	−3.70	0.03	0.360	1.000
116	AABR07065531.5	6	133667975..133676907	0.94	1.66	3.15	0.00	0.060	1.000
117	AABR07065645.1	6	complement(138549564..138550576)	0.80	−4.19	−18.29	0.00	0.060	1.000
118	AABR07065673.1	6	139158334..139158765	0.40	5.75	53.78	0.04	0.430	1.000
119	AABR07065693.3	6	139405966..139406844	10.78	−1.85	−3.61	0.00	0.000	0.001
120	AABR07065714.1	6	complement(139783839..139911839)	1.56	−8.54	−372.10	0.01	0.120	1.000
121	AABR07065750.3	6	complement(140150433..140150729)	7.67	7.55	187.68	0.00	0.000	0.000
122	AABR07065766.1	6	complement(140399800..140400347)	0.48	2.84	7.14	0.02	0.300	1.000
123	AABR07065768.1	6	complement(140485452..140485913)	3.18	2.94	7.69	0.00	0.000	0.000
124	AABR07065768.2	6	complement(140416650..140418831)	0.37	5.47	44.20	0.05	0.490	1.000
125	AABR07065778.2	6	complement(141008140..141008427)	4.37	4.40	21.11	0.00	0.000	0.000
126	AABR07065789.2	6	complement(141364359..141365198)	0.24	−3.34	−10.14	0.02	0.280	1.000
127	AABR07065792.1	6	complement(141487555..141488290)	4.35	3.77	13.63	0.00	0.000	0.000
128	AABR07065814.2	6	complement(142352806..142353308)	0.40	2.27	4.81	0.04	0.400	1.000
129	AABR07065815.1	6	complement(142584754..142585188)	3.59	5.90	59.68	0.00	0.000	0.000
130	AABR07065815.2	6	complement(142635329..142676432)	3.85	2.83	7.11	0.00	0.000	0.000
131	AABR07065823.1	6	complement(142932678..142933110)	1.15	1.79	3.46	0.00	0.100	1.000
132	AABR07065823.2	6	complement(142903147..142903440)	20.31	4.46	22.02	0.00	0.000	0.000
133	AABR07065827.1	6	complement(143065206..143065639)	2.18	2.80	6.98	0.00	0.001	0.610
134	AABR07065837.1	6	complement(143194515..143195445)	37.77	7.25	152.57	0.00	0.000	0.000
135	AABR07065883.2	6	complement(143599063..143605685)	0.44	−2.34	−5.06	0.04	0.410	1.000
136	AABR07065886.1	6	complement(143701600..143702033)	5.28	9.44	693.46	0.00	0.020	1.000
137	AABR07065889.2	6	complement(143749414..143757128)	0.57	1.96	3.89	0.03	0.350	1.000
138	AABR07066792.1	9	complement(16347795..16351503)	0.21	−6.25	−76.11	0.05	0.480	1.000
139	AABR07067082.1	9	complement(29584375..29585448)	0.47	−1.74	−3.34	0.03	0.320	1.000
140	AABR07070161.4	8	complement(55542447..55544874)	0.85	1.19	2.28	0.00	0.040	1.000
141	AABR07070238.3	8	60701256..60702538	2.42	2.72	6.61	0.00	0.000	0.000
142	AABR07072262.1	11	85561460..85562032	0.90	3.11	8.63	0.00	0.090	1.000
143	AABR07072671.1	2	2694480..2695683	1.56	2.88	7.36	0.00	0.000	0.000
144	Aass	4	complement(50152452..50200328)	0.22	−5.25	−38.15	0.00	0.010	1.000
145	Aatk	10	complement(109188112..109224370)	0.19	−1.27	−2.42	0.00	0.030	1.000
146	Abat	10	complement(7093405..7200499)	11.33	1.59	3.02	0.00	0.000	0.000
147	Abca17	10	complement(13630097..13716931)	0.06	−2.49	−5.61	0.00	0.060	1.000
148	Abcd2	7	complement(132294562..132343169)	0.09	−1.66	−3.16	0.01	0.120	1.000
149	Abcg5	6	complement(7935771..7961207)	0.41	−1.22	−2.33	0.00	0.050	1.000
150	Abo	3	complement(4374602..4394428)	1.30	−3.31	−9.90	0.00	0.000	0.000
151	AC094053.1	2	complement(104996717..105001497)	0.15	−8.02	−259.10	0.01	0.190	1.000
152	AC098008.2	1	167672812..167673926	2.80	−1.16	−2.23	0.00	0.000	0.060
153	AC099449.1	1	complement(215908373..215986959)	0.31	−1.61	−3.06	0.02	0.250	1.000
154	AC107331.1	1	170075617..170081138	0.19	−1.15	−2.21	0.00	0.060	1.000
155	AC109096.1	1	complement(220836092..220836504)	6.14	−1.29	−2.45	0.00	0.009	1.000
156	AC109901.1	11	85618714..85619188	1.29	2.92	7.58	0.00	0.070	1.000
157	AC111231.1	2	150061636..150081281	0.69	3.29	9.81	0.00	0.000	0.100
158	AC114343.1	15	28028521..28035389	0.14	−6.47	−88.45	0.04	0.440	1.000
159	AC114446.1	7	140608434..140609067	0.34	−2.34	−5.08	0.01	0.230	1.000
160	AC126292.2	5	135687538..135687897	0.66	−3.87	−14.65	0.01	0.110	1.000
161	AC127640.1	1	complement(75455599..75462453)	0.18	−1.05	−2.06	0.02	0.240	1.000
162	AC128394.1	1	12906899..12908181	2.05	2.65	6.29	0.00	0.000	0.000
163	AC141028.2	X	complement(5789401..5824651)	0.72	6.93	122.26	0.01	0.200	1.000
164	Acsbg2	9	10172832..10203460	0.06	−2.54	−5.80	0.05	0.470	1.000
165	Adam18	16	72139063..72210900	0.40	1.28	2.43	0.00	0.020	1.000
166	Adam28	15	complement(50358192..50425900)	0.22	−2.03	−4.09	0.01	0.120	1.000
167	Adam32	16	71889235..71999148	0.07	−3.53	−11.52	0.02	0.270	1.000
168	Adamts13	3	5519990..5558166	0.22	−1.65	−3.14	0.00	0.005	1.000
169	Adamts15	8	complement(31977001..32000378)	0.76	−1.70	−3.24	0.00	0.000	0.000
170	Adamts5	11	complement(25410975..25456836)	0.46	−1.09	−2.12	0.00	0.030	1.000
171	Adcy1	14	87312203..87421659	0.42	−1.81	−3.51	0.00	0.000	0.200
172	Adcy2	1	37507276..37698390	5.73	1.42	2.67	0.00	0.000	0.000
173	Adh7	2	243502073..243516532	0.07	−2.24	−4.73	0.05	0.470	1.000
174	Adipoq	11	complement(81330293..81344488)	2.33	−5.43	−43.20	0.00	0.000	0.000
175	Adm2	7	130296897..130298644	1.57	−1.13	−2.20	0.00	0.010	1.000
176	Adrb2	18	complement(57513793..57515834)	32.79	1.00	2.00	0.00	0.000	0.000
177	Agbl2	3	79613171..79646698	0.47	−1.72	−3.28	0.00	0.002	0.970
178	Agmo	6	56846789..57193961	0.09	−2.28	−4.85	0.02	0.270	1.000
179	Agt	19	complement(57321640..57333433)	0.37	−1.56	−2.95	0.00	0.030	1.000
180	Ak5	2	complement(257671772..257864385)	0.08	−1.58	−2.98	0.03	0.400	1.000
181	Akap3	4	159403501..159425629	0.08	−2.55	−5.86	0.01	0.210	1.000
182	Akap4	X	complement(16296735..16306078)	0.10	−6.32	−80.04	0.05	0.480	1.000
183	Akap6	6	73553210..73990534	0.03	−1.63	−3.10	0.03	0.350	1.000
184	Akap7	1	21141970..21263850	2.45	−1.24	−2.36	0.00	0.000	0.060
185	Akr1c12	17	complement(69695550..69711689)	0.48	2.78	6.85	0.00	0.002	1.000
186	Aldh1a3	1	complement(127301128..127337882)	4.56	−1.04	−2.05	0.00	0.000	0.001
187	Aldh1l2	7	26375866..26425108	1.12	−1.39	−2.62	0.00	0.000	0.010
188	Angpt1	7	complement(81342280..81592206)	0.24	−2.08	−4.22	0.00	0.050	1.000
189	Angptl1	13	74410010..74474681	0.66	−2.43	−5.38	0.00	0.003	1.000
190	Ankrd29	18	complement(3673915..3676188)	0.20	−1.60	−3.03	0.01	0.150	1.000
191	Ankrd31	2	27630285..27742903	0.14	1.43	2.69	0.02	0.310	1.000
192	Anlnl1	17	66548818..66551947	0.32	1.70	3.24	0.00	0.007	1.000
193	Ano1	1	complement(217754336..217902473)	4.65	−1.43	−2.70	0.00	0.000	0.000
194	Anxa3	14	complement(14364008..14426437)	2.66	−1.09	−2.13	0.00	0.000	0.002
195	Anxa5	2	complement(123162461..123193130)	18.90	−1.39	−2.62	0.00	0.000	0.000
196	Aox3	9	65013862..65099433	2.05	−3.12	−8.69	0.00	0.000	0.000
197	Apln	X	complement(134856726..134866210)	0.28	−1.02	−2.03	0.01	0.190	1.000
198	Apobec2	9	14529218..14542731	0.30	1.38	2.60	0.02	0.320	1.000
199	Apod	11	72705129..72726301	0.33	−1.69	−3.23	0.01	0.230	1.000
200	App	11	complement(24425005..24641858)	41.13	−1.14	−2.21	0.00	0.000	0.000
201	Aqp7	5	complement(57358327..57372239)	0.35	−3.94	−15.32	0.00	0.008	1.000
202	Arhgef33	6	3012804..3095500	0.48	−1.38	−2.61	0.00	0.010	1.000
203	Armcx6	X	complement(105565747..105568343)	0.32	−1.06	−2.09	0.02	0.310	1.000
204	Arrdc2	16	20352480..20356612	14.04	−1.40	−2.64	0.00	0.000	0.000
205	Asf1b	19	25077918..25092491	0.34	1.31	2.48	0.01	0.200	1.000
206	Aspa	10	complement(59839852..59892960)	0.81	1.30	2.46	0.00	0.001	0.270
207	Aspn	17	complement(14656009..14679409)	0.27	−7.27	−154.48	0.02	0.290	1.000
208	Atp12a	15	36565495..36590171	0.92	−10.47	−1419.79	0.00	0.030	1.000
209	Atp2c2	19	52347580..52404607	0.95	1.62	3.08	0.00	0.000	0.000
210	Atp6v0d2	5	complement(33843519..33892462)	0.88	−1.07	−2.09	0.00	0.070	1.000
211	Atp6v1c2	6	complement(42585934..42630983)	28.58	−1.67	−3.18	0.00	0.000	0.000
212	Atp7b	16	74865516..74945286	0.11	−1.98	−3.94	0.00	0.009	1.000
213	Azin2	5	complement(147120129..147148291)	0.24	−1.69	−3.24	0.01	0.140	1.000
214	Bbs7	2	complement(123283388..123323170)	0.45	−1.12	−2.18	0.00	0.040	1.000
215	Bche	2	complement(171100140..171196395)	0.14	−1.97	−3.92	0.00	0.001	0.680
216	Bcl3	1	complement(80730499..80744831)	0.96	−1.03	−2.04	0.00	0.020	1.000
217	Bmp15	X	17016778..17023418	0.08	−1.52	−2.86	0.04	0.420	1.000
218	Bmp3	14	complement(12359363..12387102)	0.09	−1.80	−3.48	0.00	0.070	1.000
219	Bmp6	17	complement(26955142..27112820)	17.45	−6.26	−76.59	0.00	0.000	0.000
220	Bmp8a	5	complement(141007462..141033511)	0.21	2.41	5.30	0.00	0.010	1.000
221	Bmp8b	5	140803638..140820628	0.13	2.07	4.21	0.03	0.350	1.000
222	Brms1	1	220423426..220426975	1.74	8.90	477.87	0.00	0.040	1.000
223	Btnl2	20	4141878..4154978	0.16	−1.47	−2.78	0.03	0.380	1.000
224	Btnl3	20	4156858..4171887	0.20	−1.51	−2.85	0.01	0.160	1.000
225	C1galt1	4	33890349..33943211	4.51	1.24	2.36	0.00	0.000	0.000
226	C4a_1	20	complement(2651599..2678141)	2.11	−1.15	−2.22	0.00	0.000	0.000
227	C4bpb	13	complement(47387173..47397890)	0.36	1.17	2.26	0.05	0.480	1.000
228	Capn8	13	100980574..101043110	0.03	−2.89	−7.41	0.04	0.460	1.000
229	Car2	2	complement(88097720..88113029)	37.22	−4.51	−22.83	0.00	0.000	0.000
230	Car3	2	complement(88126667..88135410)	11.07	−3.40	−10.59	0.00	0.000	0.000
231	Cbr1_2	11	33845463..33847793	2.56	−4.06	−16.71	0.00	0.000	0.000
232	Cbs	20	complement(10361988..10386751)	30.34	1.02	2.02	0.00	0.000	0.000
233	Ccdc136	4	56674832..56704549	0.62	−1.22	−2.33	0.00	0.002	1.000
234	Ccl19	5	complement(58181026..58183017)	1.66	−1.21	−2.31	0.01	0.130	1.000
235	Ccl20	9	88918433..88921001	0.57	1.04	2.06	0.05	0.470	1.000
236	Ccl21	5	complement(58197680..58198782)	5.58	−1.35	−2.55	0.00	0.000	0.000
237	Ccl9	10	complement(70783424..70788309)	0.10	−3.00	−8.01	0.04	0.410	1.000
238	Ccnj	1	260093641..260111254	0.07	1.98	3.95	0.01	0.140	1.000
239	Ccser1	4	91373942..91972325	0.08	−1.38	−2.61	0.05	0.490	1.000
240	Cct8l1_1	4	complement(6044792..6046477)	0.32	2.40	5.27	0.00	0.050	1.000
241	Cd248	1	220353356..220355650	0.27	7.88	235.95	0.00	0.090	1.000
242	Cd52	5	complement(152322916..152324469)	4.34	−4.79	−27.74	0.00	0.000	0.002
243	Cd69	4	complement(163041141..163049084)	0.47	1.62	3.07	0.00	0.020	1.000
244	Cdh12	2	72006099..72540263	0.36	1.82	3.53	0.00	0.000	0.000
245	Cdkl4	6	complement(3234090..3254779)	0.98	1.62	3.08	0.00	0.001	0.610
246	Cdo1	18	complement(40701984..40716686)	1.36	−1.98	−3.95	0.00	0.000	0.000
247	Ceacam1	1	complement(82327955..82344345)	6.91	−1.90	−3.73	0.00	0.000	0.000
248	Cers3	1	127648234..127781017	0.09	−2.88	−7.35	0.00	0.050	1.000
249	Ces1f	19	15081158..15335002	0.29	2.07	4.19	0.00	0.000	0.000
250	Cfap126	13	89480058..89495949	0.09	−2.89	−7.40	0.05	0.470	1.000
251	Cfap45	13	90909486..90932961	0.14	−1.69	−3.22	0.02	0.280	1.000
252	Cfap77	3	complement(7508496..7632345)	0.16	−4.19	−18.26	0.00	0.060	1.000
253	Cfd	7	complement(12634216..12635943)	7.34	−1.07	−2.10	0.00	0.000	0.000
254	Cga	5	50381244..50393367	0.40	−1.89	−3.70	0.02	0.240	1.000
255	Chchd2	9	complement(101388151..101388833)	2.02	2.36	5.12	0.00	0.050	1.000
256	Chl1	4	70252366..70330803	0.28	1.16	2.24	0.00	0.040	1.000
257	Chrdl2	1	165008912..165036435	0.29	−1.32	−2.49	0.02	0.300	1.000
258	Chst7	X	complement(2623024..2657155)	0.28	−1.18	−2.26	0.02	0.240	1.000
259	Cidec	4	complement(145377431..145390497)	0.63	−5.08	−33.92	0.00	0.000	0.000
260	Cilp	8	70760922..70775891	1.60	−1.45	−2.74	0.00	0.000	0.000
261	Ckap2	16	74752655..74779184	0.60	1.29	2.45	0.01	0.120	1.000
262	Cks2	17	complement(13587665..13593423)	1.23	1.13	2.19	0.01	0.150	1.000
263	Clca4	2	complement(250843165..250862419)	0.20	−2.06	−4.16	0.00	0.030	1.000
264	Clec4a2	4	complement(155792761..155867708)	0.25	1.37	2.59	0.02	0.310	1.000
265	Clu	15	42640146..42665857	302.28	−2.02	−4.04	0.00	0.000	0.000
266	Cntd1	10	89199880..89209393	0.14	2.45	5.48	0.01	0.100	1.000
267	Col5a3	8	complement(21786324..21831668)	0.63	−1.24	−2.36	0.00	0.000	0.000
268	Col8a1	11	44877859..45007891	0.64	−1.20	−2.30	0.00	0.001	0.530
269	Colec12	18	867048..1052606	22.35	−1.20	−2.30	0.00	0.000	0.000
270	Cpn1	1	complement(263733519..263762785)	0.47	−1.80	−3.48	0.00	0.005	1.000
271	Cpne5	20	complement(6419033..6500523)	0.06	−2.58	−6.00	0.01	0.190	1.000
272	Cpz	14	80403001..80426245	0.42	−1.57	−2.97	0.00	0.010	1.000
273	Crabp1	8	59344083..59352132	0.13	−2.89	−7.40	0.05	0.470	1.000
274	Crim1	6	788548..960703	3.18	−1.43	−2.69	0.00	0.000	0.000
275	Crisp2	9	23503236..23527496	0.32	−7.30	−157.78	0.02	0.310	1.000
276	Crnkl1_2	6	complement(18349167..18351536)	0.84	1.34	2.52	0.00	0.000	0.130
277	Crym	1	complement(189944895..189960073)	0.48	−1.15	−2.22	0.02	0.300	1.000
278	Cryz	2	260884337..260911792	0.23	−1.19	−2.28	0.04	0.400	1.000
279	Csap1	10	13230158..13230575	20.90	−1.65	−3.13	0.00	0.000	0.000
280	Cst5	3	144569502..144574112	3.38	−3.13	−8.78	0.00	0.000	0.000
281	Cxcl6	14	complement(18860264..18862407)	0.11	−3.54	−11.63	0.01	0.190	1.000
282	Cyp11a1	8	62779875..62809893	0.40	−2.63	−6.18	0.00	0.000	0.090
283	Cyp2b2	1	83103925..83119193	0.30	7.40	168.93	0.01	0.130	1.000
284	Cyp2c24	1	258074860..258139176	0.50	1.88	3.68	0.00	0.000	0.100
285	Cyp2d5	7	complement(123616596..123621102)	0.13	1.60	3.04	0.04	0.400	1.000
286	Cyp4a8	5	complement(133978954..134008255)	0.54	1.92	3.78	0.00	0.000	0.050
287	Cyp7b1	2	complement(102701903..102871257)	28.09	1.26	2.40	0.00	0.000	0.000
288	Cyss	3	complement(144427207..144431806)	4.72	−1.37	−2.58	0.01	0.200	1.000
289	Dbil5	10	64375924..64376938	0.36	−1.77	−3.41	0.03	0.370	1.000
290	Dcdc2	17	complement(41838201..42031265)	0.25	−2.76	−6.76	0.00	0.000	0.000
291	Dclk3	8	119691388..119744506	0.08	−1.47	−2.78	0.03	0.380	1.000
292	Defb1	16	complement(75294376..75309176)	1.27	−3.78	−13.69	0.00	0.008	1.000
293	Defb50	16	complement(75249704..75262002)	116.91	−7.77	−217.65	0.00	0.000	0.000
294	Dgki	4	complement(64374342..64831473)	0.25	−1.09	−2.13	0.00	0.100	1.000
295	Dhrs9	3	55623634..55648325	0.12	1.04	2.06	0.05	0.480	1.000
296	Dkk2	2	237148941..237241350	0.72	−1.65	−3.14	0.00	0.000	0.000
297	Dlg2	1	156552328..157269747	0.07	−1.75	−3.35	0.04	0.460	1.000
298	Dnah17	10	complement(107029466..107158997)	0.18	1.55	2.94	0.00	0.000	0.000
299	Dner	9	complement(91965866..92291220)	0.04	−1.89	−3.71	0.05	0.490	1.000
300	Dok6	18	complement(86420361..86878142)	1.97	2.05	4.13	0.00	0.000	0.000
301	Dpep3	19	complement(37946722..37952501)	0.13	−2.23	−4.70	0.01	0.220	1.000
302	Dpysl5	6	complement(26939697..27024129)	0.02	−3.00	−8.01	0.04	0.420	1.000
303	Dscaml1	8	49737798..50055560	0.06	−2.50	−5.67	0.00	0.030	1.000
304	Dsel	13	complement(1942499..1946508)	3.36	1.47	2.77	0.00	0.000	0.000
305	Dusp15	3	complement(148417993..148428494)	0.05	−2.08	−4.24	0.04	0.430	1.000
306	Dusp26	16	complement(64798778..64806050)	0.30	−1.03	−2.05	0.03	0.380	1.000
307	E2f8	1	complement(104187100..104202591)	0.10	1.74	3.33	0.01	0.200	1.000
308	Edn1	17	complement(22137324..22143324)	0.71	−1.70	−3.24	0.00	0.010	1.000
309	Efcab12	4	complement(147721045..147742114)	0.36	−1.64	−3.13	0.00	0.030	1.000
310	Efcab3	10	93305969..93332702	0.92	−1.02	−2.03	0.00	0.100	1.000
311	Efhc2	X	5825711..6026398	2.06	4.62	24.67	0.00	0.000	0.000
312	Efnb2	16	86631064..86675049	1.45	−1.06	−2.09	0.00	0.000	0.040
313	Elmod1	8	complement(58486078..58542844)	0.15	−7.00	−128.09	0.03	0.350	1.000
314	Enam	14	complement(21194664..21219454)	7.66	1.33	2.51	0.00	0.000	0.000
315	Eno2	4	complement(157285179..157294047)	0.87	−1.16	−2.23	0.01	0.160	1.000
316	Entpd7_1	1	262987957..263000254	0.47	−1.66	−3.17	0.03	0.330	1.000
317	Epb41l4a	18	complement(26570647..26658892)	0.31	−1.13	−2.18	0.02	0.240	1.000
318	Epha4	9	complement(83111222..83253458)	0.10	−1.08	−2.12	0.02	0.280	1.000
319	Ephx2	15	complement(42757235..42794279)	0.74	−1.31	−2.49	0.00	0.007	1.000
320	Erich5	7	73222730..73242899	0.22	−1.36	−2.57	0.05	0.480	1.000
321	Espl1	7	143897014..143923868	0.17	1.28	2.43	0.00	0.030	1.000
322	Esr2	6	complement(99164357..99214251)	12.22	−1.42	−2.68	0.00	0.000	0.000
323	Esrrg	13	106463368..106683436	0.14	−1.03	−2.04	0.01	0.140	1.000
324	Etnk2	13	complement(50481163..50499140)	1.06	−1.06	−2.08	0.00	0.003	1.000
325	Exoc7	10	complement(105163754..105182834)	0.35	2.19	4.55	0.01	0.170	1.000
326	Eya4	1	23409408..23610164	0.11	−1.58	−2.99	0.05	0.470	1.000
327	Fabp4	2	93792601..93797305	10.87	−1.43	−2.69	0.00	0.000	0.000
328	Fam111a	1	229003961..229019527	54.01	−7.51	−182.83	0.00	0.000	0.000
329	Fam189a2	1	complement(241822626..241875864)	0.38	−1.34	−2.53	0.01	0.170	1.000
330	Fam227b	3	complement(118268739..118427851)	0.25	−1.16	−2.23	0.03	0.400	1.000
331	Fam229b	20	complement(44207478..44220702)	0.33	−3.88	−14.73	0.01	0.210	1.000
332	Fam25a	16	complement(10702263..10706073)	5.04	−2.14	−4.40	0.00	0.000	0.000
333	Fam3d	15	18322327..18355818	0.64	−1.93	−3.82	0.00	0.002	1.000
334	Fam71f1	4	56591715..56615158	0.06	−3.20	−9.21	0.03	0.340	1.000
335	Fancd2	4	145489869..145551479	0.19	1.18	2.26	0.00	0.070	1.000
336	Fbxl7	2	complement(79067915..79078258)	0.16	−1.05	−2.07	0.02	0.300	1.000
337	Fcamr	13	47539231..47552848	0.07	1.50	2.84	0.04	0.430	1.000
338	Fcgbpl1	1	85058548..85096346	0.16	−1.94	−3.84	0.01	0.130	1.000
339	Fhl4	7	complement(26139664..26144466)	0.22	−1.27	−2.41	0.04	0.430	1.000
340	Fosl1	1	220826560..220835066	0.36	−1.18	−2.27	0.02	0.270	1.000
341	Foxi2	1	207654487..207656138	0.15	−2.60	−6.04	0.02	0.280	1.000
342	Frzb	3	complement(67635607..67668772)	0.23	−1.26	−2.39	0.01	0.150	1.000
343	G0s2	13	complement(112004140..112005052)	0.64	−1.32	−2.49	0.02	0.270	1.000
344	Gapdhs	1	complement(89180063..89194602)	0.31	−4.06	−16.69	0.00	0.020	1.000
345	Garem2	6	complement(27631364..27643076)	0.13	1.24	2.36	0.05	0.470	1.000
346	Gata6	18	2416552..2446338	0.15	−1.41	−2.65	0.03	0.370	1.000
347	Gnal	18	62805410..62944630	11.03	−1.58	−2.99	0.00	0.000	0.150
348	Gng4	17	complement(90266794..90315492)	0.65	2.50	5.66	0.00	0.000	0.000
349	Gp2	1	complement(189166399..189182306)	0.04	2.61	6.10	0.02	0.290	1.000
350	Gpr137c	15	19547871..19614805	0.12	−1.89	−3.71	0.05	0.480	1.000
351	Gpr150	2	complement(2812554..2814690)	1.80	1.79	3.45	0.00	0.000	0.000
352	Gpr179	10	complement(85274057..85289777)	0.36	−2.25	−4.76	0.00	0.000	0.000
353	Gpr27	4	132137793..132138926	0.09	−2.89	−7.40	0.05	0.470	1.000
354	Gpr37	4	complement(51822153..51844331)	0.05	−2.06	−4.17	0.03	0.360	1.000
355	Gpx3	10	40247436..40255422	32.31	−1.14	−2.21	0.00	0.000	0.000
356	Grhl3	5	complement(153893039..153924896)	0.50	−2.76	−6.79	0.00	0.001	0.260
357	Grid2	4	94696965..95442778	0.03	−2.24	−4.73	0.04	0.450	1.000
358	Gsg1	4	complement(169020683..169036950)	0.54	−4.12	−17.36	0.00	0.006	1.000
359	Gspt2	X	complement(63954727..63961613)	0.52	−1.96	−3.89	0.00	0.000	0.000
360	Gstm6	2	complement(210733594..210738378)	13.48	1.17	2.25	0.00	0.000	0.000
361	Gucy2c	4	complement(170659998..170740274)	0.05	−3.58	−11.92	0.02	0.250	1.000
362	Gucy2g	1	complement(276187242..276228574)	1.66	−2.27	−4.83	0.00	0.000	0.000
363	Gys2	4	complement(176638629..176679815)	0.24	3.94	15.34	0.00	0.000	0.070
364	Gzmb	15	complement(35413793..35417316)	0.11	−1.83	−3.55	0.02	0.320	1.000
365	Habp2	1	277068761..277104566	0.45	1.24	2.36	0.00	0.060	1.000
366	Haus1	18	complement(74141481..74151950)	0.70	2.71	6.54	0.00	0.060	1.000
367	Havcr1	10	31813814..31848379	0.06	−2.22	−4.65	0.01	0.220	1.000
368	Hemgn	5	complement(62005071..62025152)	0.06	−2.34	−5.05	0.04	0.430	1.000
369	Hhat	13	complement(111236466..111474411)	0.62	−1.12	−2.17	0.01	0.120	1.000
370	Hhipl1	6	132219088..132240356	0.14	−1.21	−2.31	0.04	0.460	1.000
371	Hist1h2bk_2	17	complement(44527421..44527801)	1.08	−4.64	−24.92	0.00	0.020	1.000
372	Hjurp	9	complement(95349086..95362014)	0.23	1.11	2.16	0.01	0.190	1.000
373	Hmgb1	12	7081895..7094002	1.56	1.43	2.69	0.00	0.009	1.000
374	Hmgn5	X	complement(80134557..80142902)	0.41	−8.06	−267.65	0.01	0.170	1.000
375	Hnrnpa3_2	10	80953006..80954332	1.57	−1.84	−3.59	0.00	0.060	1.000
376	Hoga1	1	261291870..261318984	10.28	−1.15	−2.22	0.00	0.000	0.000
377	Hoxb9	10	84119884..84123390	0.54	−4.06	−16.66	0.00	0.007	1.000
378	Hpgd	16	complement(37457135..37495758)	0.77	−1.14	−2.21	0.01	0.120	1.000
379	Hprt1	X	complement(158197149..158228749)	0.32	6.30	78.80	0.03	0.360	1.000
380	Hrasls	11	complement(75125952..75144903)	0.12	−2.37	−5.16	0.01	0.180	1.000
381	Hrasls5	1	222907159..222937633	0.15	−6.33	−80.29	0.05	0.490	1.000
382	Hsd11b2	19	37476095..37481307	0.10	−1.52	−2.86	0.04	0.430	1.000
383	Hsd3b2	2	complement(200585748..200762492)	0.18	−4.95	−30.90	0.00	0.010	1.000
384	Hsf2bp	20	complement(10757854..10844178)	4.20	1.76	3.39	0.00	0.000	0.000
385	Hsp90aa1	6	complement(135045363..135049728)	0.89	−9.46	−703.35	0.00	0.070	1.000
386	Hspa12a	1	complement(279946811..280015358)	0.17	−1.06	−2.08	0.01	0.120	1.000
387	Hspa1b	20	complement(4877324..4879779)	84.26	1.19	2.28	0.00	0.000	0.000
388	Hspb9	10	88620655..88621300	0.47	−1.56	−2.96	0.04	0.450	1.000
389	Htr5b	13	complement(37479758..37492680)	2.90	1.42	2.67	0.00	0.000	0.000
390	Hyal1	8	116332796..116335435	2.12	1.12	2.17	0.00	0.000	0.000
391	Icam5	8	22050222..22057209	0.09	−2.25	−4.75	0.01	0.160	1.000
392	Ifitm2	1	complement(213750192..213751405)	93.40	−1.21	−2.31	0.00	0.000	0.000
393	Igh-6	6	complement(138092131..138093643)	53.32	1.09	2.13	0.00	0.000	0.000
394	Ighg	6	complement(139140679..139142218)	118.66	1.41	2.66	0.00	0.000	0.000
395	Ighv12-3	6	complement(142177345..142178771)	0.48	1.77	3.41	0.03	0.390	1.000
396	Ighv8-4	6	complement(143590014..143590448)	2.29	3.50	11.33	0.00	0.000	0.050
397	Il12rb2	4	complement(98052842..98141482)	0.46	−1.84	−3.57	0.00	0.000	0.230
398	Il15ra	17	complement(70451411..70481750)	1.69	−1.57	−2.97	0.00	0.000	0.000
399	Il1rapl2	X	108287068..109851047	0.04	2.61	6.10	0.02	0.290	1.000
400	Il23r	4	complement(98203958..98305173)	0.13	1.59	3.00	0.03	0.380	1.000
401	Il36rn	3	1385654..1392275	0.08	−2.30	−4.91	0.01	0.130	1.000
402	Inhba	17	complement(51898217..51912496)	0.20	−1.80	−3.48	0.00	0.060	1.000
403	Insrr	2	187162017..187181395	0.14	−1.32	−2.49	0.00	0.050	1.000
404	Ipcef1	1	complement(43567842..43638161)	0.12	1.63	3.09	0.04	0.410	1.000
405	Irak1bp1	8	90343154..90360086	0.89	−1.59	−3.01	0.00	0.030	1.000
406	Irgc	1	complement(81291765..81295442)	0.37	−1.71	−3.27	0.00	0.080	1.000
407	Jph2	3	complement(159712325..159775643)	1.08	−1.08	−2.11	0.00	0.003	1.000
408	Ka11	10	complement(88141667..88144625)	0.30	1.13	2.19	0.04	0.450	1.000
409	Kcna7	1	101397828..101403320	0.24	1.48	2.80	0.00	0.005	1.000
410	Kcnc3	1	100593680..100607874	0.31	1.00	2.00	0.00	0.010	1.000
411	Kcng1	3	complement(165020230..165039707)	0.10	−2.83	−7.13	0.00	0.050	1.000
412	Kcng3	6	complement(6794808..6842758)	0.70	1.78	3.43	0.00	0.001	0.250
413	Kcnh1	13	110920737..111232269	0.08	−1.25	−2.39	0.04	0.410	1.000
414	Kcnk3	6	complement(27151612..27190132)	0.33	−1.04	−2.05	0.00	0.030	1.000
415	Kcnq1	1	216293087..216630339	0.27	−1.24	−2.36	0.00	0.050	1.000
416	Kcns3	6	complement(36764044..36819821)	0.30	−1.19	−2.28	0.01	0.140	1.000
417	Kcnt1	3	3310954..3365340	0.06	−1.26	−2.39	0.05	0.480	1.000
418	Kif17	5	156628432..156666284	0.11	−1.21	−2.31	0.05	0.490	1.000
419	Kif27	17	6701315..6772349	0.15	−1.11	−2.16	0.01	0.130	1.000
420	Klf8	X	complement(18835827..18890090)	0.31	−2.30	−4.92	0.00	0.000	0.000
421	Klk1c2	1	complement(99981045..99985422)	21.24	−3.42	−10.70	0.00	0.000	0.000
422	Knstrn	3	110618298..110638046	0.51	1.15	2.22	0.01	0.190	1.000
423	Krt23	10	complement(87391623..87407634)	0.71	−1.83	−3.55	0.00	0.005	1.000
424	Krt4	7	complement(143518010..143523503)	0.15	−6.53	−92.71	0.04	0.410	1.000
425	Krt7	7	143059764..143075907	3.43	−1.54	−2.90	0.00	0.000	0.000
426	Lamc2	13	complement(70566643..70626252)	13.12	1.08	2.11	0.00	0.000	0.000
427	Ldhc	1	102915191..102931839	0.16	−2.85	−7.20	0.02	0.250	1.000
428	Lep	4	56337695..56351818	0.29	−8.31	−318.23	0.01	0.140	1.000
429	Lgals4	1	87019975..87027691	0.37	1.34	2.53	0.03	0.340	1.000
430	Lingo3	7	11737293..11746703	0.34	1.01	2.02	0.02	0.300	1.000
431	Lipo1	1	complement(252102219..252116971)	0.05	2.07	4.21	0.02	0.300	1.000
432	Lmtk3	1	101783621..101792496	0.18	1.12	2.18	0.05	0.480	1.000
433	LOC100271845	1	196737627..196740027	0.17	−4.69	−25.82	0.00	0.040	1.000
434	LOC100359539	19	25391700..25394229	2.10	1.26	2.39	0.00	0.000	0.060
435	LOC100359951	9	complement(1012091..1012450)	1.20	2.00	4.01	0.00	0.060	1.000
436	LOC100360117	7	117967818..117969850	8.02	−3.78	−13.70	0.00	0.000	0.000
437	LOC100360218	X	122724129..122781479	0.29	−2.25	−4.75	0.00	0.060	1.000
438	LOC100360581	6	139560028..139560489	2.47	−2.78	−6.89	0.00	0.003	1.000
439	LOC100360690	1	complement(252154616..252167752)	18.32	1.89	3.71	0.00	0.000	0.000
440	LOC100361105	6	complement(141146719..141147264)	1.56	1.92	3.78	0.00	0.005	1.000
441	LOC100361706_1	11	86092468..86092779	23.64	1.91	3.77	0.00	0.000	0.000
442	LOC100361706_2	11	86094567..86096397	3.05	3.41	10.65	0.00	0.000	0.000
443	LOC100362684	5	161889342..161889701	1.29	2.54	5.82	0.00	0.020	1.000
444	LOC100362814	3	complement(94064234..94182714)	0.32	−1.01	−2.01	0.00	0.070	1.000
445	LOC100363452	3	103192683..103193333	1.24	−4.85	−28.75	0.00	0.006	1.000
446	LOC100366231	1	146976975..146989858	0.19	3.00	7.98	0.00	0.040	1.000
447	LOC100909485	X	complement(17962909..17964888)	0.17	−1.96	−3.88	0.02	0.300	1.000
448	LOC100909505	7	63467216..63499353	0.32	7.81	224.65	0.01	0.130	1.000
449	LOC100909657	4	149908375..149908980	2.00	−1.00	−2.00	0.01	0.110	1.000
450	LOC100909840	2	complement(147955196..148050438)	0.57	−1.30	−2.47	0.01	0.170	1.000
451	LOC100909856	5	142332607..142376370	0.30	2.50	5.67	0.00	0.020	1.000
452	LOC100909913	X	6791136..6815583	0.13	−1.57	−2.97	0.02	0.310	1.000
453	LOC100910237	1	complement(22757012..22758012)	1.85	−4.80	−27.85	0.00	0.000	0.000
454	LOC100910438	2	28370373..28381311	0.62	1.15	2.22	0.04	0.420	1.000
455	LOC100910528	1	complement(32570296..32570589)	0.71	6.30	78.80	0.03	0.360	1.000
456	LOC100910650	11	71211768..71216983	1.61	−1.48	−2.79	0.00	0.070	1.000
457	LOC100910732	11	72829238..72865902	0.15	−6.71	−104.47	0.04	0.410	1.000
458	LOC100910792	17	47721977..47817248	0.22	−1.11	−2.16	0.01	0.180	1.000
459	LOC100910979_1	18	55463308..55464632	0.25	−2.08	−4.22	0.02	0.230	1.000
460	LOC100911186	1	complement(212570195..212579057)	12.19	−1.49	−2.80	0.00	0.000	0.000
461	LOC100911225	1	complement(212563714..212568224)	0.12	−1.38	−2.61	0.04	0.410	1.000
462	LOC100911337_3	4	complement(120413653..120414118)	21.11	−2.98	−7.88	0.00	0.050	1.000
463	LOC100911374	11	complement(72786730..72814850)	0.26	6.84	114.17	0.02	0.230	1.000
464	LOC100911453	2	208420206..208586356	0.09	2.51	5.71	0.04	0.430	1.000
465	LOC100911515	9	20213588..20217176	0.36	−1.29	−2.44	0.01	0.220	1.000
466	LOC100911615	1	213997709..214002815	1.43	−4.85	−28.75	0.00	0.000	0.030
467	LOC100911625	9	20251521..20260505	0.23	7.67	203.82	0.01	0.140	1.000
468	LOC100911727	1	75233703..75264293	0.06	5.77	54.61	0.05	0.470	1.000
469	LOC100911769	3	complement(153843206..153893847)	0.59	−1.09	−2.13	0.00	0.003	1.000
470	LOC100911837	11	complement(85802605..85895613)	0.11	−7.53	−184.45	0.02	0.240	1.000
471	LOC100911881	1	complement(214039481..214074663)	0.09	6.13	70.14	0.03	0.360	1.000
472	LOC100912034	4	31387420..31392856	0.15	−2.19	−4.57	0.01	0.150	1.000
473	LOC100912498	11	complement(82875627..82884660)	0.54	5.92	60.74	0.00	0.000	0.130
474	LOC100912611	5	146318568..146321925	0.13	1.98	3.96	0.01	0.190	1.000
475	LOC100912707	3	16413080..16413632	2.25	1.40	2.64	0.05	0.490	1.000
476	LOC102549061	17	43627930..43628310	1.20	2.02	4.07	0.00	0.060	1.000
477	LOC102549115	1	88451958..88453578	0.65	8.61	389.63	0.00	0.050	1.000
478	LOC102550734	8	complement(104155401..104155775)	0.42	2.34	5.07	0.02	0.250	1.000
479	LOC102553099	11	complement(61466962..61470348)	0.67	−6.94	−122.54	0.03	0.380	1.000
480	LOC102553715_1	10	47765432..47768483	13.32	−1.12	−2.18	0.00	0.000	0.000
481	LOC102554500	3	4988097..4989205	0.19	5.93	61.08	0.04	0.410	1.000
482	LOC102555392	18	55466373..55467629	6.37	−1.34	−2.52	0.00	0.000	0.000
483	LOC103689941	5	142303636..142330854	0.08	6.18	72.27	0.03	0.380	1.000
484	LOC103689993	X	complement(45244038..45259492)	1201.74	−2.95	−7.71	0.00	0.000	0.000
485	LOC103690005	1	91042635..91048483	0.97	−2.32	−4.99	0.00	0.003	1.000
486	LOC103690016	1	197659187..197681006	0.22	8.36	328.71	0.00	0.080	1.000
487	LOC103690020	4	14212925..14241272	0.14	2.10	4.30	0.00	0.070	1.000
488	LOC103690114	1	87790104..87801872	0.10	6.80	111.31	0.01	0.210	1.000
489	LOC103690137	3	50656125..50658546	0.10	−2.17	−4.51	0.03	0.400	1.000
490	LOC103690354	15	28074953..28075411	0.50	−2.95	−7.71	0.01	0.130	1.000
491	LOC103691013	12	21767606..21773959	0.14	−1.89	−3.69	0.01	0.230	1.000
492	LOC103692167_1	4	113910685..113913687	0.59	1.07	2.10	0.01	0.190	1.000
493	LOC103694381	20	5184515..5186503	0.28	7.11	137.98	0.01	0.170	1.000
494	LOC108348067	1	220362064..220364358	0.20	7.45	174.93	0.01	0.130	1.000
495	LOC108348083	1	complement(259641676..259674425)	6.86	−1.44	−2.71	0.00	0.000	0.000
496	LOC108348085	1	220322940..220324430	0.64	3.97	15.70	0.00	0.000	0.030
497	LOC108348114	5	129043675..129082442	1.69	2.09	4.25	0.01	0.220	1.000
498	LOC108348137	X	105911925..105915821	0.84	−1.07	−2.10	0.00	0.010	1.000
499	LOC108348142	7	118507224..118509256	8.27	−3.82	−14.13	0.00	0.000	0.000
500	LOC108348189	7	117963740..117964414	1.93	−1.97	−3.91	0.00	0.040	1.000
501	LOC108352650	13	91334004..91334306	1.08	−3.47	−11.06	0.00	0.080	1.000
502	LOC292543	1	64618506..64632131	0.74	−1.08	−2.12	0.00	0.020	1.000
503	LOC298795	6	23757225..23758496	0.36	−2.26	−4.80	0.00	0.030	1.000
504	LOC300308_1	7	20262680..20480453	0.03	−3.03	−8.18	0.04	0.450	1.000
505	LOC360919	14	complement(19028810..19072677)	0.22	−1.00	−2.00	0.05	0.490	1.000
506	LOC365778	2	complement(127770767..127778350)	0.14	−2.79	−6.93	0.00	0.030	1.000
507	LOC500712	6	127941526..128080889	1.00	−1.89	−3.71	0.00	0.000	0.000
508	LOC679794	10	complement(36501996..36502313)	0.29	2.94	7.66	0.05	0.470	1.000
509	LOC682793	14	104452917..104453129	1.74	−7.03	−131.11	0.03	0.350	1.000
510	LOC685963	8	63119395..63119607	1.64	−6.95	−123.84	0.03	0.370	1.000
511	LOC687679	4	118167294..118174842	10.03	−1.43	−2.69	0.00	0.000	0.000
512	LOC687707	10	57239993..57240947	0.51	−2.10	−4.28	0.00	0.090	1.000
513	LOC687880	1	complement(99299011..99303048)	3.56	4.00	16.05	0.00	0.000	0.000
514	LOC688754	18	complement(51613210..51614057)	0.33	−1.84	−3.57	0.01	0.190	1.000
515	LOC689899	14	24129592..24130122	0.24	2.61	6.10	0.02	0.290	1.000
516	LOC689986	14	complement(3248080..3288017)	0.09	1.91	3.76	0.04	0.430	1.000
517	LOC690468_1	3	164002482..164002694	1.38	−6.71	−104.47	0.04	0.410	1.000
518	LOC690507	3	149802621..149812183	0.11	−3.38	−10.44	0.02	0.270	1.000
519	LOC690700	14	94590765..94602088	0.34	−1.98	−3.94	0.04	0.410	1.000
520	LOC691352	10	complement(44238354..44243402)	0.35	−7.22	−148.81	0.02	0.300	1.000
521	LOC691931	2	complement(232214331..232245319)	2.77	−1.07	−2.09	0.00	0.004	1.000
522	Lpar5	4	157594436..157595972	0.24	−1.24	−2.36	0.04	0.430	1.000
523	Lpl	16	complement(22537056..22561496)	2.21	−2.35	−5.11	0.00	0.000	0.000
524	Lrrc15	11	74050166..74051902	0.39	−1.35	−2.55	0.00	0.090	1.000
525	Lrrc34	2	116372519..116391959	0.40	−1.21	−2.32	0.00	0.100	1.000
526	Lrrc74b	11	complement(87418205..87434482)	0.22	−1.57	−2.97	0.03	0.320	1.000
527	Lta	20	4852496..4854677	0.08	2.61	6.10	0.02	0.290	1.000
528	Ltbp2	6	complement(108500112..108596569)	1.53	−1.45	−2.73	0.00	0.000	0.000
529	Ltc4s	10	complement(35737664..35739625)	0.69	−1.28	−2.44	0.04	0.410	1.000
530	Ly6g6e	20	5067330..5068624	0.99	−1.08	−2.11	0.04	0.410	1.000
531	M6pr	4	155088317..155092827	0.35	−7.01	−128.69	0.03	0.360	1.000
532	Mageb16	X	complement(46977655..47078128)	0.14	−1.84	−3.57	0.02	0.240	1.000
533	March10	10	complement(93591188..93679974)	0.07	−6.37	−82.70	0.05	0.480	1.000
534	Mas1	1	48077033..48108216	0.27	1.13	2.19	0.02	0.310	1.000
535	Masp1	11	80736576..80803382	0.71	−1.09	−2.12	0.00	0.003	1.000
536	Mb21d1	8	complement(85789763..85803433)	0.14	−1.89	−3.70	0.03	0.340	1.000
537	Mboat1	17	complement(36061775..36177457)	0.84	−1.05	−2.07	0.00	0.090	1.000
538	Mc5r	18	64114933..64116524	1.29	1.62	3.07	0.00	0.000	0.001
539	Mcoln2	2	252018597..252068271	2.43	−3.51	−11.38	0.00	0.000	0.010
540	Mcoln3	2	251983339..252007560	0.49	−1.48	−2.79	0.01	0.140	1.000
541	Mcpt2	15	34862266..35034622	0.42	2.05	4.14	0.00	0.000	0.050
542	Mdh1b	9	complement(70404093..70450170)	0.07	−2.06	−4.17	0.03	0.360	1.000
543	Meiob	10	14174732..14206025	0.14	−1.65	−3.14	0.04	0.410	1.000
544	Meltf	11	complement(72135668..72157522)	0.05	−2.53	−5.76	0.01	0.130	1.000
545	Mest	4	58053041..58063138	0.27	−1.89	−3.70	0.02	0.260	1.000
546	MGC105567	18	55685613..55704904	1.68	1.15	2.23	0.00	0.000	0.020
547	MGC94199_2	5	24146171..24165463	1.34	−1.50	−2.84	0.05	0.470	1.000
548	Mgmt	1	209237233..209464190	9.97	−1.18	−2.26	0.00	0.000	0.000
549	Mgst1	4	172119331..172134607	22.14	−1.23	−2.34	0.00	0.000	0.000
550	Miga1	2	complement(257493875..257546799)	0.42	−1.25	−2.37	0.00	0.050	1.000
551	Mip	7	2635743..2642848	0.08	−1.65	−3.13	0.05	0.470	1.000
552	Mmd	10	77755216..77783779	13.93	−1.37	−2.59	0.00	0.000	0.000
553	Mmp7	8	5893249..5901049	7.09	−2.87	−7.30	0.00	0.000	0.000
554	Morn4	1	complement(261323083..261333383)	2.86	−1.93	−3.81	0.00	0.000	0.000
555	Mpo	10	75087892..75098260	0.13	−6.99	−126.89	0.03	0.330	1.000
556	Mpz	13	89524329..89530068	1.36	−1.08	−2.11	0.00	0.003	1.000
557	Mr1	13	complement(72771984..72789841)	0.41	−1.02	−2.03	0.03	0.390	1.000
558	Mrap	11	30904733..30915225	0.19	−3.21	−9.22	0.02	0.320	1.000
559	Mroh2a	9	95309759..95347384	0.04	−1.63	−3.10	0.03	0.370	1.000
560	Ms4a14	1	complement(227471436..227486976)	0.08	−1.38	−2.60	0.03	0.350	1.000
561	Msi1	12	complement(46895447..46920952)	0.12	−1.45	−2.73	0.03	0.360	1.000
562	Msln	10	complement(15119663..15125408)	0.67	−2.67	−6.36	0.00	0.000	0.000
563	Msmb	16	8280275..8300852	115.92	−8.43	−344.01	0.00	0.000	0.000
564	Mt1	17	78793336..78793724	25.04	−3.17	−8.99	0.00	0.000	0.000
565	Mt2A	19	complement(11307967..11308740)	20.87	−2.53	−5.76	0.00	0.000	0.002
566	Mt3	19	complement(11324698..11326139)	3.06	−6.33	−80.32	0.00	0.001	0.670
567	Mtcl1	9	complement(114491419..114619711)	0.53	−1.63	−3.09	0.00	0.000	0.000
568	Muc1	2	188543137..188547874	0.39	−1.13	−2.19	0.00	0.080	1.000
569	Muc20	11	71222600..71236223	0.18	−2.69	−6.45	0.00	0.080	1.000
570	Mug1	4	154391647..154729310	0.06	1.96	3.88	0.02	0.240	1.000
571	Myh6	15	complement(33605654..33629699)	0.06	−3.13	−8.73	0.00	0.020	1.000
572	Mzb1	18	complement(28436548..28438654)	1.09	1.30	2.46	0.00	0.030	1.000
573	Ncam1	8	complement(53839098..53901358)	0.55	−1.40	−2.64	0.00	0.000	0.050
574	Nckap5	13	complement(42265806..42885440)	0.04	−2.14	−4.39	0.01	0.200	1.000
575	Nek5	16	74810938..74848113	0.10	−3.54	−11.63	0.01	0.190	1.000
576	NEWGENE_1565644	X	144807392..144807829	19.07	3.00	8.00	0.00	0.000	0.000
577	Nfe2l3_2	4	81205404..81232813	0.39	−2.08	−4.22	0.00	0.006	1.000
578	Nkx3-1	15	51065316..51068421	5.25	−3.44	−10.84	0.00	0.000	0.001
579	Nlrc3	10	11810926..11842482	0.16	1.01	2.01	0.04	0.430	1.000
580	Nlrc4	6	22167919..22194250	0.28	−1.43	−2.70	0.00	0.020	1.000
581	Nme5	18	complement(27357413..27374603)	0.28	−1.60	−3.02	0.01	0.150	1.000
582	Nnat	3	154043873..154046330	3.60	−1.33	−2.51	0.00	0.000	0.000
583	Nnmt	8	complement(52925175..52937972)	0.46	−1.44	−2.71	0.01	0.220	1.000
584	Nov	7	94375020..94383024	4.94	2.05	4.15	0.00	0.000	0.000
585	Nptx1	10	complement(108682638..108691367)	0.18	−1.34	−2.52	0.00	0.040	1.000
586	Nqo2	17	complement(32132347..32158538)	1.45	−3.78	−13.73	0.00	0.000	0.000
587	Nr1i2	11	65022100..65058545	0.68	1.07	2.10	0.00	0.020	1.000
588	Nrg1	16	63837216..64057434	0.10	−1.62	−3.06	0.01	0.100	1.000
589	Nrgn	8	complement(40015049..40023193)	0.36	1.51	2.85	0.01	0.120	1.000
590	Nt5c1b	6	36089433..36106849	0.08	−3.41	−10.65	0.02	0.310	1.000
591	Ntf3	4	complement(158636884..158705886)	0.28	−1.16	−2.23	0.04	0.410	1.000
592	Ntsr1	3	175885894..176046345	0.07	−1.97	−3.92	0.01	0.140	1.000
593	Nubpl	6	72891725..73148536	111.41	1.01	2.01	0.00	0.000	0.000
594	Nuggc	15	49010492..49055237	0.08	1.82	3.52	0.02	0.290	1.000
595	Nupr1l1	1	complement(198043073..198045154)	0.99	−7.86	−233.01	0.01	0.230	1.000
596	Oas1g	12	41155497..41167505	2.11	1.04	2.06	0.00	0.000	0.010
597	Oas1i	12	42343123..42355131	0.42	−7.91	−240.29	0.01	0.220	1.000
598	Oaz3	2	complement(195676048..195678848)	0.88	−4.46	−21.94	0.00	0.020	1.000
599	Ocm2	12	12624723..12634472	49.33	−1.56	−2.95	0.00	0.000	0.000
600	Odf1	7	77066955..77074711	0.78	−5.55	−46.82	0.00	0.010	1.000
601	Ogn	17	complement(15447716..15467359)	3.38	−2.07	−4.20	0.00	0.000	0.000
602	Olr1029	7	complement(7753040..7753978)	0.14	5.62	49.17	0.05	0.460	1.000
603	Olr657	3	complement(77388589..77389521)	0.26	1.66	3.16	0.02	0.250	1.000
604	Olr956	7	complement(6753411..6754349)	0.20	3.09	8.49	0.01	0.110	1.000
605	Osbp2	14	complement(83943977..84106997)	4.12	2.15	4.45	0.00	0.000	0.000
606	Oscar	1	64182422..64188658	0.08	2.45	5.47	0.02	0.250	1.000
607	Otx1	14	complement(106861676..106864892)	3.38	1.04	2.05	0.00	0.000	0.160
608	P2rx5	10	59725398..59737126	0.10	−1.74	−3.33	0.02	0.250	1.000
609	Parpbp	7	complement(28654707..28715224)	0.06	1.36	2.56	0.04	0.410	1.000
610	Pbld1	20	complement(27103317..27117663)	1.08	1.37	2.59	0.00	0.000	0.008
611	Pbsn	X	complement(45178446..45187149)	76.89	−2.82	−7.06	0.00	0.000	0.000
612	Pcdh10	2	136993208..137014492	19.34	−4.17	−17.99	0.00	0.000	0.000
613	Pcdhga5	18	30840868..30843291	0.06	−1.89	−3.71	0.05	0.480	1.000
614	Pcdhgb6	18	30890869..30893286	0.29	−1.04	−2.05	0.01	0.140	1.000
615	Pck1	3	171213936..171219871	0.48	−8.76	−432.81	0.01	0.100	1.000
616	Pcsk2	3	137618898..137925605	0.03	5.83	56.87	0.04	0.440	1.000
617	Pcsk9	5	complement(126031368..126053726)	2.48	2.37	5.16	0.00	0.000	0.000
618	Pde1b	7	145117951..145145376	0.17	−1.67	−3.18	0.02	0.250	1.000
619	Pde1c	4	complement(86359820..86630543)	0.12	−1.42	−2.68	0.01	0.210	1.000
620	Pde5a	2	226900619..227041576	2.10	−1.07	−2.10	0.00	0.000	0.000
621	Pde8b	2	complement(24719976..24923128)	0.07	−3.21	−9.23	0.03	0.330	1.000
622	Pdia5	11	68493035..68578995	18.95	−1.67	−3.19	0.00	0.000	0.000
623	Peli2	15	24942218..25083931	0.28	−1.34	−2.53	0.00	0.020	1.000
624	Pidd1	1	complement(214419240..214423881)	0.14	7.20	147.41	0.01	0.190	1.000
625	Pla2g2a	5	157282669..157285328	11.27	−1.12	−2.18	0.00	0.000	0.002
626	Pla2g2d	5	157222636..157228791	1.81	1.13	2.18	0.00	0.008	1.000
627	Plcl1	9	62291405..62349309	0.19	−1.04	−2.06	0.01	0.130	1.000
628	Plekhs1	1	277261681..277291370	1.39	−1.77	−3.42	0.00	0.000	0.000
629	Plet1	8	54925607..54937177	1.84	−1.10	−2.14	0.00	0.010	1.000
630	Plin1	1	complement(141458181..141471010)	0.77	−6.28	−77.93	0.00	0.000	0.000
631	Plin4	9	10963723..10971277	0.68	−1.60	−3.03	0.00	0.000	0.000
632	Pllp	19	10731855..10752641	1.09	−1.20	−2.30	0.00	0.050	1.000
633	Plppr4	2	220298245..220341866	0.04	−6.37	−82.70	0.05	0.480	1.000
634	Plxna3	X	complement(156363405..156379189)	0.32	−1.04	−2.06	0.00	0.006	1.000
635	Plxnc1	7	complement(35850398..36000906)	1.13	1.54	2.90	0.00	0.000	0.000
636	Pmfbp1	19	complement(42132527..42180981)	0.17	−1.32	−2.50	0.01	0.180	1.000
637	Pon3	4	complement(30311982..30338679)	0.76	−2.35	−5.08	0.00	0.000	0.050
638	Popdc3	20	50394650..50422560	8.24	1.23	2.34	0.00	0.000	0.000
639	Pparg	4	147274107..147399380	0.16	−1.61	−3.05	0.01	0.200	1.000
640	Prelid2	18	complement(36194778..36285444)	0.09	−1.23	−2.35	0.04	0.410	1.000
641	Prm1	10	4945911..4946397	1.29	−7.49	−179.61	0.02	0.280	1.000
642	Prox1	13	108382399..108443294	0.04	−1.40	−2.65	0.03	0.380	1.000
643	Prr19	1	82169620..82172636	0.14	−2.67	−6.38	0.01	0.220	1.000
644	Prr30	6	26780352..26782864	0.10	−3.60	−12.15	0.02	0.260	1.000
645	Prss39	9	41006800..41013965	0.14	−1.53	−2.88	0.02	0.300	1.000
646	Psapl1	14	complement(79711694..79714254)	0.26	1.35	2.56	0.00	0.080	1.000
647	Ptma	13	complement(89873243..89874008)	4.23	−1.47	−2.77	0.00	0.001	0.600
648	Qpct	6	1657331..1689559	10.26	−1.16	−2.24	0.00	0.000	0.000
649	Rab15	6	complement(99845954..99870024)	29.80	−1.00	−2.00	0.00	0.000	0.000
650	Rab17	9	complement(98118908..98131883)	0.50	−1.11	−2.17	0.01	0.130	1.000
651	Rab37	10	103703404..103710527	0.17	2.15	4.44	0.01	0.160	1.000
652	Rab3c	2	complement(41570597..41785792)	6.27	1.67	3.17	0.00	0.000	0.000
653	Rab6b	8	111600532..111668808	3.20	−1.27	−2.42	0.00	0.000	0.000
654	Rasd1	10	complement(46330368..46332172)	11.29	−1.28	−2.43	0.00	0.000	0.000
655	Rasl11a	12	complement(9985928..9990284)	0.42	−1.05	−2.08	0.03	0.380	1.000
656	Rassf6	14	18983853..19025971	0.30	−1.08	−2.11	0.00	0.050	1.000
657	Rbm12_2	3	complement(152176095..152179193)	0.10	6.86	116.47	0.02	0.240	1.000
658	Rbp4	1	complement(256806472..256813711)	1.01	−5.84	−57.47	0.00	0.001	0.590
659	Rbp7	5	complement(166278187..166282831)	0.39	−2.08	−4.23	0.01	0.220	1.000
660	Rbpms2	8	71167305..71200536	0.38	−1.56	−2.94	0.00	0.006	1.000
661	Rec114	8	complement(63445644..63533546)	0.31	−3.32	−10.02	0.00	0.050	1.000
662	Retn	12	2201891..2204249	0.34	−7.20	−147.21	0.02	0.300	1.000
663	Rfesd	2	complement(2769400..2779109)	28.33	1.54	2.91	0.00	0.000	0.000
664	Rfx4	7	25808419..25907849	0.96	1.41	2.65	0.00	0.000	0.150
665	Rgcc	15	complement(61551861..61564695)	2.67	−1.43	−2.70	0.00	0.000	0.020
666	RGD1305184	18	55666027..55667668	1.83	1.44	2.71	0.00	0.000	0.002
667	RGD1306227	2	52333396..52355042	0.40	−1.09	−2.12	0.00	0.100	1.000
668	RGD1308065	11	88131960..88145481	0.06	−2.89	−7.40	0.05	0.470	1.000
669	RGD1309170	2	complement(243447168..243475639)	0.06	−3.11	−8.61	0.03	0.360	1.000
670	RGD1309362	18	55505993..55508704	3.00	−1.35	−2.55	0.00	0.000	0.000
671	RGD1311084	3	complement(9629842..9636058)	0.29	−1.45	−2.73	0.01	0.210	1.000
672	RGD1559441	5	24860153..24908397	0.10	−1.07	−2.10	0.04	0.450	1.000
673	RGD1559972	11	complement(82366074..82366505)	1.21	−4.23	−18.71	0.00	0.020	1.000
674	RGD1560281	12	20667601..20677689	0.04	−2.75	−6.72	0.01	0.200	1.000
675	RGD1560821	X	77885586..77886170	0.56	−6.60	−96.80	0.04	0.420	1.000
676	RGD1561143	12	complement(21866077..21923281)	0.29	1.41	2.66	0.03	0.340	1.000
677	RGD1561777	20	complement(45042770..45053640)	0.18	2.66	6.32	0.01	0.140	1.000
678	RGD1563231	3	complement(16999132..16999720)	11.30	1.55	2.92	0.00	0.000	0.000
679	RGD1563570	14	43694183..43694611	0.47	−3.76	−13.52	0.01	0.180	1.000
680	RGD1563705	8	complement(118378029..118378460)	0.41	−2.72	−6.60	0.03	0.340	1.000
681	RGD1565170	15	37418885..37419358	0.38	3.92	15.10	0.00	0.100	1.000
682	RGD1565355	4	14071507..14113255	0.18	−2.37	−5.17	0.00	0.050	1.000
683	RGD1565617_1	3	complement(19320304..19320915)	34.85	8.03	261.64	0.00	0.000	0.000
684	Rgs1	13	complement(61066153..61070599)	0.74	1.30	2.47	0.00	0.030	1.000
685	Rln1	1	complement(247483302..247486202)	0.45	1.24	2.36	0.04	0.410	1.000
686	Rmt1	1	complement(218369204..218374619)	0.27	−8.70	−416.30	0.01	0.110	1.000
687	Rn50_10_0891.3	10	89173843..89208961	0.06	−1.52	−2.88	0.03	0.340	1.000
688	Rn50_11_0160.1	11	12275964..12277727	2.03	−1.78	−3.42	0.00	0.000	0.000
689	Rn50_2_2279.2	2	complement(208555595..208557785)	0.61	−6.48	−89.40	0.04	0.420	1.000
690	Rn50_2_2279.4	2	complement(208397955..208400158)	1.26	1.22	2.32	0.02	0.300	1.000
691	Rn50_4_1360.2	4	complement(71275061..71286669)	0.07	−6.18	−72.32	0.05	0.490	1.000
692	Rn60_3_0143.1	3	14192061..14202271	0.56	6.08	67.86	0.03	0.400	1.000
693	Rn60_5_1207.2	5	120498890..120682283	0.07	−3.16	−8.94	0.00	0.090	1.000
694	Rnf144a	6	complement(45509787..45629168)	0.24	−1.15	−2.21	0.00	0.060	1.000
695	Rnf150	19	24044103..24265444	0.46	−1.75	−3.35	0.00	0.001	0.310
696	Rnf157	10	complement(105293490..105368242)	0.91	1.43	2.70	0.00	0.000	0.030
697	Romo1	3	151688454..151689711	17.14	1.55	2.93	0.00	0.000	0.000
698	Rpl10l	6	complement(88231611..88232252)	0.69	−4.00	−15.96	0.00	0.008	1.000
699	Rpl3l	10	14094754..14105308	0.13	−3.30	−9.83	0.02	0.290	1.000
700	Rps4x_1	4	complement(35729628..35730599)	4.24	1.32	2.49	0.00	0.050	1.000
701	RT1-CE9-ps1	20	5351611..5354095	7.06	−1.12	−2.18	0.00	0.000	0.000
702	RT1-DMa	20	complement(5240975..5244386)	0.26	−1.56	−2.95	0.05	0.490	1.000
703	RT1-M2	20	complement(1337149..1339488)	0.27	−2.62	−6.13	0.00	0.050	1.000
704	Runx1t1	5	27312928..27442841	0.09	−1.17	−2.25	0.02	0.250	1.000
705	Runx2	9	18564927..18773092	3.72	1.09	2.13	0.00	0.000	0.000
706	S100a11	2	193866951..193868502	24.65	−1.36	−2.56	0.00	0.000	0.001
707	S100b	20	complement(13130636..13142856)	2.08	−1.95	−3.86	0.00	0.000	0.000
708	Sarm1	10	complement(65742343..65766050)	0.17	1.03	2.04	0.02	0.240	1.000
709	Sat2	10	56227692..56229850	0.91	−1.16	−2.24	0.00	0.060	1.000
710	Scara3	15	complement(42605473..42638392)	1.10	−1.45	−2.74	0.00	0.000	0.001
711	Sccpdh	13	97941757..97963824	1.52	5.75	53.73	0.00	0.000	0.010
712	Scube3	20	7718282..7746331	0.08	−1.58	−2.98	0.03	0.390	1.000
713	Sel1l3	14	60123169..60229734	0.21	−1.23	−2.35	0.00	0.080	1.000
714	Selenow	1	complement(77533148..77535681)	3.58	−1.72	−3.30	0.03	0.380	1.000
715	Sema5b	11	complement(68306659..68349238)	0.05	−2.43	−5.39	0.03	0.350	1.000
716	Semg1	3	complement(160627610..160630457)	610.95	−8.95	−495.61	0.00	0.000	0.000
717	Serpina1	6	complement(127610243..127632265)	0.23	−2.00	−3.99	0.01	0.140	1.000
718	Serpinb7	13	27312498..27354052	0.92	−1.35	−2.55	0.00	0.020	1.000
719	Sh2d4b	16	17611115..17708387	0.24	1.21	2.31	0.04	0.440	1.000
720	Sh3gl3	1	144069638..144200397	0.11	−2.34	−5.08	0.01	0.210	1.000
721	Siglec5	1	complement(98562346..98570949)	0.21	−1.61	−3.04	0.00	0.080	1.000
722	Six2	6	complement(8952572..8956276)	1.50	1.05	2.07	0.00	0.004	1.000
723	Slc13a3	3	complement(162084315..162147393)	0.30	1.07	2.10	0.00	0.100	1.000
724	Slc13a4	4	complement(62797677..62840357)	0.20	1.73	3.31	0.01	0.110	1.000
725	Slc18b1	1	complement(22705916..22748422)	3.81	−3.61	−12.18	0.00	0.000	0.000
726	Slc22a25	1	complement(224596887..224698514)	0.17	1.81	3.51	0.00	0.070	1.000
727	Slc25a21	6	complement(77624385..77848434)	0.10	−1.46	−2.75	0.05	0.490	1.000
728	Slc25a45	1	221236773..221244563	0.47	−1.37	−2.58	0.00	0.010	1.000
729	Slc28a3	17	6459056..6509799	0.22	−1.26	−2.40	0.02	0.240	1.000
730	Slc29a2	1	220400855..220407189	0.86	−8.72	−422.32	0.01	0.110	1.000
731	Slc2a12	1	complement(24003625..24056373)	1.59	−1.61	−3.05	0.00	0.000	0.000
732	Slc2a5	5	167141875..167174310	0.64	2.10	4.27	0.00	0.000	0.140
733	Slc30a2	5	152559577..152571799	8.21	−4.21	−18.55	0.00	0.000	0.000
734	Slc38a11	3	complement(51371923..51416646)	1.49	−5.63	−49.45	0.00	0.000	0.009
735	Slc44a4	20	4593389..4609631	30.06	1.74	3.34	0.00	0.000	0.000
736	Slc4a4	14	complement(20479323..20920286)	0.06	−1.97	−3.92	0.01	0.160	1.000
737	Slc5a11	1	193187972..193226649	4.20	−6.58	−95.60	0.00	0.000	0.000
738	Slc6a4	10	complement(63153651..63176463)	0.36	−1.06	−2.09	0.00	0.090	1.000
739	Slc7a10	1	91433030..91448564	0.11	−2.75	−6.72	0.01	0.210	1.000
740	Slc7a13	5	33784715..33805616	0.40	−1.57	−2.98	0.00	0.010	1.000
741	Slc9a2	9	47386626..47491907	0.94	1.22	2.33	0.00	0.002	1.000
742	Slfnl1	5	139468546..139473017	0.15	−1.43	−2.70	0.04	0.410	1.000
743	Slit2	14	complement(66831848..67170361)	0.25	−1.30	−2.47	0.00	0.002	1.000
744	Smad9	2	144191846..144212138	0.03	−3.38	−10.42	0.02	0.240	1.000
745	Smcp	2	complement(192775437..192780631)	0.64	−7.56	−189.32	0.02	0.270	1.000
746	Sncg	16	complement(10722106..10726707)	4.36	−3.57	−11.84	0.00	0.000	0.000
747	Snrpn	1	complement(195074330..195096460)	0.35	−1.05	−2.07	0.05	0.470	1.000
748	Snx31	7	complement(75309255..75364166)	0.17	−1.83	−3.55	0.02	0.300	1.000
749	Sostdc1	6	55812747..55817066	0.15	−1.42	−2.67	0.04	0.440	1.000
750	Spag6	17	85382116..85437975	0.08	−2.24	−4.73	0.04	0.460	1.000
751	Spata18	14	complement(37081427..37108341)	0.17	−7.01	−128.69	0.03	0.360	1.000
752	Spata6	5	131719922..131803853	1.93	−1.06	−2.09	0.00	0.000	0.010
753	Spata9	2	2729829..2766853	5.10	1.31	2.47	0.00	0.000	0.000
754	Spc25	3	complement(55438536..55451798)	0.35	1.25	2.38	0.03	0.360	1.000
755	Spp1	14	complement(6673686..6679901)	0.58	−1.31	−2.47	0.00	0.030	1.000
756	Sptb	6	complement(99659651..99783047)	0.81	−1.06	−2.08	0.00	0.000	0.003
757	Srm	5	165405168..165408320	8.26	−1.55	−2.93	0.00	0.000	0.000
758	Srsf12	5	48456757..48485912	12.93	3.62	12.29	0.00	0.000	0.000
759	St18	5	complement(12437525..12563429)	0.07	−1.74	−3.33	0.01	0.200	1.000
760	St3gal1	7	complement(107895045..107963142)	3.29	1.96	3.89	0.00	0.000	0.000
761	Stag3	12	19599834..19630780	0.05	−3.10	−8.55	0.01	0.150	1.000
762	Sult5a1	19	complement(55952853..55967836)	0.80	−1.26	−2.40	0.00	0.040	1.000
763	Susd4	13	101181994..101307908	0.26	−1.46	−2.74	0.00	0.020	1.000
764	Syne4l1	1	88772904..88776999	0.71	−1.48	−2.78	0.01	0.160	1.000
765	Syngr1	7	121311024..121334437	6.79	−1.20	−2.29	0.00	0.000	0.000
766	Syt1	7	complement(50084060..50638798)	4.85	3.66	12.67	0.00	0.000	0.000
767	Tbxa2r	7	complement(11253180..11257977)	6.35	1.05	2.08	0.00	0.000	0.000
768	Tcaf2	4	complement(71206876..71229575)	1.76	−1.62	−3.06	0.00	0.000	0.120
769	Tcerg1l	1	complement(210550668..210739600)	10.38	1.73	3.31	0.00	0.000	0.000
770	Tcf23	6	complement(26762761..26771164)	0.08	−1.25	−2.37	0.04	0.450	1.000
771	Tcp10b	1	53531076..53554113	0.11	−6.44	−86.70	0.04	0.450	1.000
772	Tcp11	20	complement(7645626..7654428)	0.19	−7.00	−128.09	0.03	0.350	1.000
773	Tdrd1	1	277641512..277685257	0.03	−1.98	−3.94	0.04	0.440	1.000
774	Tdrd6	9	19917603..19932920	0.03	−6.37	−82.43	0.05	0.470	1.000
775	Tfrc	11	complement(71397383..71419223)	8.25	1.03	2.04	0.00	0.000	0.000
776	tGap1	2	92549479..92625948	0.08	−1.52	−2.87	0.04	0.400	1.000
777	Thrsp	1	complement(162381253..162385575)	1.77	−3.70	−12.97	0.00	0.000	0.000
778	Tle2	7	complement(10988532..11003785)	0.54	−1.55	−2.93	0.00	0.003	1.000
779	Tmc5	1	188278469..188337308	0.07	−2.18	−4.53	0.01	0.100	1.000
780	Tmeff2	9	complement(55384100..55673829)	0.09	−1.28	−2.43	0.04	0.440	1.000
781	Tmem179	6	complement(137073685..137084739)	0.10	−3.87	−14.65	0.01	0.110	1.000
782	Tmem182	9	47536824..47588677	0.06	−3.30	−9.83	0.02	0.290	1.000
783	Tmem35b	5	145188323..145191953	0.69	−1.82	−3.53	0.00	0.010	1.000
784	Tmem59l	16	20691978..20695701	43.07	8.18	289.34	0.00	0.000	0.000
785	Tmprss13	8	49621568..49650860	0.46	−2.16	−4.48	0.00	0.000	0.010
786	Tmtc2	7	complement(47181981..47586137)	0.19	−2.08	−4.22	0.00	0.040	1.000
787	Tnik	2	113984646..114384894	0.44	1.98	3.95	0.00	0.000	0.000
788	Tnp2	10	4953879..4954624	0.99	−3.25	−9.50	0.00	0.070	1.000
789	Trbv1	4	69138525..69139130	0.39	1.84	3.57	0.04	0.460	1.000
790	Trhde	7	complement(57253023..57679795)	0.64	1.01	2.01	0.00	0.000	0.030
791	Trim63	5	152533349..152547179	0.78	−3.37	−10.32	0.00	0.000	0.002
792	Triml1	16	52169450..52177586	0.72	−1.32	−2.50	0.00	0.009	1.000
793	Trnp1	5	complement(151709200..151709877)	0.40	−1.62	−3.07	0.02	0.260	1.000
794	Tsen34l1	1	complement(64024240..64030175)	0.91	3.41	10.60	0.00	0.040	1.000
795	Tsga10ip	1	complement(220772411..220787238)	0.09	−1.88	−3.69	0.02	0.290	1.000
796	Tsnaxip1	19	37795658..37813424	0.20	−1.13	−2.18	0.03	0.360	1.000
797	Ttc23	1	128606770..128687893	2.65	−1.11	−2.16	0.00	0.000	0.001
798	Tubg2	10	89069256..89075585	0.23	−1.71	−3.28	0.01	0.170	1.000
799	Tulp2	1	101474334..101511412	0.12	−2.50	−5.67	0.02	0.280	1.000
800	Ubbp4	1	complement(255572847..255573377)	0.99	−4.86	−29.00	0.00	0.050	1.000
801	Ubd	20	complement(1876173..1897814)	12.16	−1.02	−2.03	0.00	0.020	1.000
802	Ube2d4	14	complement(2933100..2934571)	0.17	−1.52	−2.87	0.04	0.410	1.000
803	Ube3d	8	complement(93948767..94120458)	0.61	−1.12	−2.18	0.01	0.110	1.000
804	Unc5c	2	247248407..247397483	0.06	−1.01	−2.01	0.04	0.450	1.000
805	Unc5cl	9	complement(14491584..14508235)	0.02	−3.11	−8.61	0.03	0.350	1.000
806	Upk1a	1	complement(89060581..89068348)	1.81	−1.33	−2.51	0.00	0.003	1.000
807	Upk1b	11	64522130..64553228	0.16	1.77	3.41	0.01	0.140	1.000
808	Vom1r25	8	3540361..3541275	3.87	1.81	3.52	0.00	0.000	0.000
809	Vom2r-ps125	14	complement(759050..773281)	0.09	−2.09	−4.26	0.05	0.470	1.000
810	Vtcn1	2	203200427..203276142	0.52	−2.47	−5.53	0.00	0.000	0.000
811	Wdr54	4	complement(114850815..114853868)	0.30	−1.57	−2.97	0.03	0.340	1.000
812	Wdr97	7	117420788..117429504	0.09	1.09	2.12	0.04	0.450	1.000
813	Wfdc18_1	10	70964396..71014423	0.27	−2.14	−4.40	0.02	0.320	1.000
814	Wnt5b	4	complement(151500957..151516894)	0.28	−1.04	−2.06	0.01	0.190	1.000
815	Wt1	3	95133713..95180564	0.11	−1.62	−3.08	0.02	0.290	1.000
816	Xrra1	1	164849974..164914499	0.18	−1.75	−3.37	0.00	0.080	1.000
817	Ybx2	10	56546710..56551863	0.38	−1.43	−2.70	0.02	0.310	1.000
818	Zfat	7	complement(109037845..109205354)	2.04	1.00	2.00	0.00	0.000	0.000
819	Zfp113	12	complement(19288403..19294888)	0.38	−1.33	−2.52	0.02	0.240	1.000
820	Zfp300	X	908044..911067	0.08	−2.14	−4.40	0.02	0.310	1.000
821	Zfp618	5	78806607..78966813	0.15	−1.07	−2.10	0.05	0.490	1.000
822	Zfp9	4	complement(150517027..150522351)	0.67	−1.19	−2.28	0.00	0.030	1.000
823	Zglp1	8	complement(22081700..22086095)	0.14	3.23	9.40	0.04	0.420	1.000
824	Zswim7	10	complement(48587119..48599208)	0.95	−1.13	−2.19	0.02	0.230	1.000

Fig. 1.

Genes involved in proliferation of epithelial cell lines. A) A mechanistic diagram of the differentially expressed genes in Esr2−/− prostate glands that are involved in proliferation of epithelial cell lines. B) Prediction legend for the representation of pathway analyses.

Fig. 2.

Genes involved in benign tumor formation. A) A mechanistic diagram of the differentially expressed genes in Esr2−/− prostate glands that are involved in benign tumor formation. B) Prediction legend for the representation of pathway analyses.

2. Experimental design, materials, and methods

2.1. Esr2-mutant rats

Holtzman Sprague-Dawley (HSD) Esr2-knockout rat model was generated by targeting exon 3 (Δ3) of the Esr2-gene as described previously [1]. Deletion of exon 3 caused a frameshift and null mutation in the ESR2 coding sequence [1]. Animals were screened for presence of the mutation by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) and primers targeting the flanking intron sequences [1]. All procedures were performed in accordance with the protocols approved by the University of Kansas Medical Center Animal Care and Use Committee.

2.2. Sample collection and processing

18-20-week-old wildtype and Esr2−/− male rats were included in this study. Animals were sacrificed, and the ventral prostate lobes were collected. Tissue samples were cut into small species, snap frozen in to liquid nitrogen, and stored at −80C until they were processed for RNA analyses. Total RNA from prostate glands was extracted using TRI Reagent (Millipore-Sigma) following the manufactures instructions and tested by Agilent Bioanalyzer before making the RNA-seq libraries as described earlier [2], [3].

2.3. Library preparation and RNA-sequencing

Approximately 500ng of prostatic total RNA was used for the RNA-seq library preparation. Libraries were prepared using a TruSeq Stranded mRNA kit (Illumina) following the manufacturer's instructions. Briefly, mRNA was enriched from total RNA by oligo-dT magnetic beads, purified and chemically fragmented. The first strand of cDNA was synthesized by using random hexamer primers and reverse transcriptase. Then double stranded cDNA (ds cDNA) was generated by removing the RNA template and synthesizing a replacement strand, incorporating dUTP in place of dTTP. ds cDNAs were purified from the second strand reaction mix by AMPure XP beads (Beckman Coulter). The cDNA ends were blunted and a poly (A) tail was added to the 3′ ends. Finally, after ligation of indexing adaptors (Illumina), the suitable DNA fragments were selected for PCR amplification for 15 cycles. Each cDNA library was prepared from three pooled RNA samples. Two replicates of cDNA libraries were prepared for each of the wildtype and Esr2−/− groups. The cDNA libraries were sequenced at the Molecular Biology Core Laboratory of Mayo Clinic (Rochester, MN).

2.4. RNA-seq data analyses

RNA-Seq data were analyzed using CLC Genomics Workbench (Qiagen Bioinformatics) as described previously [2], [3]. Briefly, clean reads were obtained by removing low quality reads through trimming. High quality reads of prostate cDNA libraries were aligned to the Rattus norvegicus genome downloaded from NCBI database (Rn6). RNA-seq data were mapped with the following parameters: (a) maximum number of allowed mismatches was 2; (b) minimum length and similarity fraction was set at 0.8; and (c) minimum number of hits per read was 10. Gene expression values were reported as RPKM (Reads Per Kilobase of transcript per Million mapped reads) [4]. Genes that were identified as being differentially expressed met the following criteria: p-Value ≤0.05 and an absolute fold change of 2.

2.5. Accession code

The Illumina HiSeq 4000 sequencing data for wildtype and Esr2−/− rat prostate transcriptome have been deposited in National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA). The SRA numbers for wildtype and Esr2−/− rat prostate are: SRR8428800, SRR8428799, SRR8428798, SRR8428797.

2.6. Pathway analysis of differentially expressed genes in Esr2−/− prostate

Differentially expressed genes (absolute fold change ≥ 2, pValue ≤0.05) in the Esr2−/− prostate gland were subjected to Ingenuity Pathway Analysis (IPA; Qiagen Bioinformatics). The pathways and genes involved in ‘Proliferation of epithelial cell lines’ are showed in Fig. 1. The genes involved in ‘Glandular intraepithelial neoplasm’, ‘Prostatic intraepithelial tumor’, and ‘Benign tumors’ pathways are shown in Fig. 2.

3. Statistical analysis

RNA-sequencing included two cDNA libraries from each group. Each library template was prepared by pooling total RNAs from three individual rats of same genotype. Differentially expressed genes were identified by CLC Genomics Workbench as described previously [2], [3].

Transparency document

The following is the transparency document related to this article: