Antibodies |
Influenza A H1N1 HA antibody |
Thermo Fisher |
RRID: AB_2552278 |
Influenza A H1N1 NA antibody |
Thermo Fisher |
RRID: AB_2549711 |
Goat Anti-Rabbit IgG (Alexa Fluor 488) |
Abcam |
RRID: AB_2630356 |
Bacterial and Virus Strains |
Rosetta DE3 |
EMD Millipore |
Cat#70956 |
Chemicals, Peptides, and Recombinant Proteins |
Alexa Fluor 555 maleimide |
Thermo Fisher |
Cat#A20346 |
Alexa Fluor 647 maleimide |
Thermo Fisher |
Cat#A20347 |
FlAsH-EDT2 |
Toronto Research Chemicals |
Cat#F335200 |
DBCO-Sulfo-Cy5 |
Jena Bioscience |
Cat#CLK-A130–1 |
3-azido-1-propanamine |
Sigma |
Cat#762016 |
Oseltamivir carboxylate |
Toronto Research Chemicals |
Cat#O700980 |
CLPETGG peptide |
Genscript |
N/A |
Coenzyme A trilithium salt |
Sigma |
Cat#C3019 |
BMPS |
Thermo Fisher |
Cat#22298 |
NH2-PEG-OH |
Rapp Polymere |
Cat#122000–2 |
NH2-PEG-NH2 |
Rapp Polymere |
Cat#112000–2 |
NH2-PEG-Biotin |
Rapp Polymere |
Cat#133000–25-20 |
Recombinant WSN HA |
Thermo Fisher |
Cat#11692V08H25 |
Protein A |
Abcam |
Cat#ab155695 |
Experimental Models: Cell Lines |
Canine: MDCK-II |
UC Berkeley Cell Culture Facility |
https://bds.berkeley.edu/facilities/cell-culture |
Human: HEK293T |
UC Berkeley Cell Culture Facility |
https://bds.berkeley.edu/facilities/cell-culture |
Recombinant DNA |
pcDNA3.1+ |
Thermo Fisher |
Cat#V79020 |
pcDNA3.1+ vPA (WSN) |
This paper |
N/A |
pcDNA3.1+ vPB1 (WSN) |
This paper |
N/A |
pcDNA3.1+ vPB2 (WSN) |
This paper |
N/A |
pcDNA3.1+ vN (WSN) |
This paper |
N/A |
pcDNA3.1+ vNP (WSN) |
This paper |
N/A |
pcDNA3.1+ vNP(FlAsH) (WSN) |
This paper |
N/A |
pcDNA3.1+ vHA (WSN) |
This paper |
N/A |
pcDNA3.1+ vHA(G5) (WSN) |
This paper |
N/A |
pcDNA3.1+ vHA(Q3) (WSN) |
This paper |
N/A |
pcDNA3.1+ vNA (WSN) |
This paper |
N/A |
pcDNA3.1+ vNA(ybbR) (WSN) |
This paper |
N/A |
pcDNA3.1+ vM (WSN) |
This paper |
N/A |
pcDNA3.1+ vM (Ud M1) |
This paper |
N/A |
pcDNA3.1+ vM(FlAsH / ybbR) (WSN) |
This paper |
N/A |
pcDNA3.1+ vM(M48) (Ud M1) |
This paper |
N/A |
pRSETa |
Thermo Fisher |
Cat#V35120 |
pRSETa-mTG(His)6
|
This paper |
N/A |
pET28b |
EMD Millipore |
Cat#69865 |
pET28b-(His)6SrtA |
This paper |
N/A |
pET28b-SFP(His)6
|
This paper |
N/A |
Software and Algorithms |
Matlab R2017b |
Mathworks |
RRID: SCR_001622 |
Other |
TransIT-293 |
Mirus |
MIR 2704 |
TPCK-treated Trypsin |
Thermo Fisher |
Cat#20233 |
Collagen |
Corning |
Cat#354249 |
Fetuin from fetal bovine serum |
Sigma |
Cat#F3004 |
Transglutaminase 2 from guinea pig liver |
Sigma |
Cat#T5398 |
Neuraminidase from Clostridium perfringens |
Sigma |
Cat#N2876 |