Figure 1.

Experimental design and spontaneous diabetes development. A: The experimental schema of enrofloxacin treatment, whereby A22Cα^−/−PI2^−/− NOD breeder mice (10 different breeder pairs were used) were treated with enrofloxacin (top) or untreated (bottom). A22Cα^−/−PI2^−/− NOD litters from these breeders were then randomly chosen and equally divided into enrofloxacin-treated or untreated groups to minimize any breeder effects. B: Diabetes incidence between different enrofloxacin treatment regimens of A22Cα^−/−PI2^−/− NOD mice outlined in A. C: Diabetes incidence of enrofloxacin-treated (from weaning) or untreated A22Cα^−/−PI2^−/− NOD mice. D: Diabetes incidence of enrofloxacin-treated (from weaning) or untreated G9Cα^−/−PI2^−/− NOD mice. Black arrows indicate the time of weaning. Statistical analysis was performed using the log-rank test.