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. 2019 Feb 22;68(5):1002–1013. doi: 10.2337/db18-0487

Figure 7.

Figure 7

Effect of enrofloxacin treatment on non-TCR-restricted polyclonal NOD mice. A: PCoA plot of β-diversity from fecal bacteria of 3- and 6-week-old NOD mice that were untreated or treated with enrofloxacin (n = 4–7 mice). B: Altered microbiota composition expressed as a percentage of relative abundance of bacterial genus. C: Absolute number of insulin B15-23–reactive CD8+ T cells gated from live single TCRβ+CD4−CD19−CD11b−CD11c− cells. D: Absolute number of CD69+ insulin B15-23–reactive CD8+ T cells gated as in C before subsequently gating on CD69. Frequency of IFN-γ–producing CD8+ T cells (gated from TCRβ+CD19−CD4−) (E), CD11c+ cells (gated from TCRβ−CD19−IAg7+CD11b−F480−) (F), and CD11b+ cells (gated from TCRβ−CD19−IAg7+CD11c−F480+) (G). All data were pooled from two independent experiments (n = 8/group). All statistical analyses were conducted using a Student t test. Data are presented as mean ± SEM.