Table 1.
Program designed for the purification of 20–25LfcinB/32–35BFII via RP-SPE. The framed fractions correspond to where the purest fraction probably elutes. The final volume of each fraction was 12 mL.
| Fraction N° | Solvent B | Purity b (%) | |
|---|---|---|---|
| % | μL | ||
| 1 | 0 | 0 | - |
| 2 | 5 | 600 | - |
| 3 | 11 | 1320 | - |
| 4 | 16 | 1920 | - |
| 5 | 17 | 2040 | 66 |
| 6 | 18 | 2160 | 96 |
| 7 | 19 | 2280 | 94 |
| 8 | 20 | 2400 | 92 |
| 9 a | 21 | 2520 | 88 |
| 10 | 22 | 2640 | 77 |
| 11 | 23 | 2760 | 23 |
| 12 | 24 | 2880 | - |
| 13 | 25 | 3000 | - |
| 14 | 50 | 6000 | - |
| 15 | 100 | 12000 | - |
a Fraction closest to calculated %Be. b Chromatographic purity.