. 2019 Mar 28;24(7):1215. doi: 10.3390/molecules24071215

Table 2.

Peptides purified by RP-SPE.

Peptide Code	Sequence	GRAVY ^a	%Ha ^a	Net Charge	t_R	Purity ^b		Purification Yield
Peptide Code	Sequence	GRAVY ^a	%Ha ^a	Net Charge	t_R	Crude	Purified	Purification Yield
1	Fc-Ahx-RLLR	N.D.	N.D.	+2	6.0	65	95	6
2	Fc-Ahx-RLLRRLLR	N.D.	N.D.	+4	7.2	77	90	28
3	AcOx-Ahx-RLLR	N.D.	N.D.	+2	4.4	46	99	10
4	KKWQWK	−2.8	33	+3	3.7	94	98	48
5	IHSMNSTIL	0.6	44	+1	4.3	71	81	71
6	PNNNKILVPK	−0.9	30	+2	3.0	71	91	60
7	LYIKGSGSTANLASSNYFPT	−0.1	30	+1	4.9	55	72	16
8	VSGLQYRVFR	−1.8	40	+2	3.6	54	92	35
9	N(Glc(Ac₄))-Ahx-RWQWRWQWR	N.D.	N.D.	N.D.	6.0	64	76	61
10	RWQWRWQWR-Ahx-N(Glc(Ac₄))	N.D.	N.D.	N.D.	6.4	60	66	70
11	N(Glc(Ac₄))-Ahx-RWQWRWQWR-Ahx-N(Glc(Ac₄))	N.D.	N.D.	N.D.	6.6	66	80	13
12	KKWQWKAKKLG	−1.8	36	+5	3.9	89	99	63
13	RRWQWRKKKLG	−2.5	27	+6	3.8	91	99	66
14	(RRWQWRKKKLG)₂-K-Ahx	−2.2	29	+13	4.4	73	93	27
15	Fc-Ahx-RRWQWR	N.D.	N.D.	+3	5.8	72	92	13
16	AcFer-Ahx-RWQWRWQWR	N.D.	N.D.	+3	6.7	70	89	15
17	RKKKMKKALQYIKLLKE	−1.2	35	+7	4.9	61	86	7
18	RYRRKKK	−3.8	0	+6	0.7	93	99	41
19	KMKKALQY	−1.1	37	+3	3.1	84	98	42
20	YIKLLKE	−0.1	42	+1	4.2	99	99	26
21	MKKALQYIKLLKE	−0.3	46	+3	5.2	86	99	28
22	FYFY	0.8	N.D.	0	5.2	57	83	7
23	KLLKKLLK	−0.1	50	+4	4.0	90	99	55
24	KLLK	−0.1	N.D.	+2	1.6	89	92	53

%HA: Percentage of hydrophobic amino acids, GRAVY: Hydrophobicity. ^a Evaluated by prediction through the use of the online computer tool APD3: Antimicrobial Peptide Calculator and Predictor (http://aps.unmc.edu/AP/). ^b Chromatographic purity measured by area percentage. AcOx: Oxolinic acid, Fc: ferrocen motif, Glc(Ac₄): Tetra acetylated Glucose, Ahx: 6-aminohexanoic residue, AcFer: Ferulic acid.