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. 2019 Apr 3;24(7):1303. doi: 10.3390/molecules24071303

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Intravital real-time confocal observation of Lipofectamine LTX-based LNPs in the blood. Using GLuc mRNA labeled with Cy5, two LNP formulations were prepared by mixing the mRNA with minimal or maximal volume of lipofectamine LTX solution that the manufacture suggests to use ((a–d) Lipo-Min and (e–h) Lipo-Max). Then, the LNPs was intravenously injected to the mice for observation of the blood vessel in the earlobes using intravital confocal microscopy. (a,c,e,g) Images 1 min after the injection. (b,d,f,h) Images 8 min after the injection. (a,b) Lipo-Min loaded with non-PEGylated mRNA. (c,d) Lipo-Min loaded with 20 PEG-OligoRNAs/mRNA. (e,f) Lipo-Max loaded with non-PEGylated mRNA. (g,h) Lipo-Max loaded with 20 PEG-OligoRNAs/mRNA.