Table 5.
Multiple reaction monitoring-optimized parameters for quantitation in rat plasma.
| Mode | Compounds | Capillary Voltage (kV) | Nebulizer Gas (L/h) | Desolvation Gas (L/h) | Cone Voltage (V) | Quantitation Transition (m/z, Collision (eV)1) | Confirmation Transition (m/z, Collision (eV) 1) |
|---|---|---|---|---|---|---|---|
| ESI+ | Gly | 2.5 | 50 | 800 | 14 | 75.97 > 30.19 (8) | 75.97 > 48.14 (6) |
| Asp | 2.5 | 50 | 800 | 12 | 133.97 > 74.03 (14) | 133.97 > 88.07 (10) | |
| Glu | 2.5 | 50 | 800 | 14 | 147.97 > 84.11 (16) | 147.97 > 130.03 (8) | |
| GABA | 2.5 | 50 | 800 | 20 | 103.97 > 86.99 (10) | 103.97 > 68.03 (14) | |
| NE | 2.5 | 50 | 800 | 6 | 169.97 > 152.04 (8) | 169.97 > 107.3 (18) | |
| ACh | 2.5 | 50 | 800 | 22 | 146.03 > 87.04 (12) | 146.01 > 60.11 (10) | |
| DA | 2.5 | 50 | 800 | 12 | 153.97 > 137 (10) | 153.97 > 91.08 (22) | |
| 5-HT | 2.5 | 50 | 800 | 10 | 176.97 > 160.02 (8) | 176.97 > 132.06 (20) | |
| 9-CRA | 3 | 40 | 500 | 20 | 301.29 > 123.17 (22) | 301.29 > 161.40 (22) | |
| ESI− | AA | 2.6 | 50 | 650 | 32 | 303.35 > 259.29 (12) | 303.35 > 205.21 (16) |
| LA | 2.8 | 40 | 500 | 36 | 279.35 > 261.23 (18) | 279.35 > 59.13 (18) | |
| cholesterol sulfate | 2.6 | 50 | 650 | 62 | 465.48 > 97.03 (36) | 465.48 > 80.00 (80) |