Table 5. Effect of 4D3 on inhibitory effect of CDDP in tumors of MKN74 and TMK-1 cells.
| TMK-1 | MKN74 | |||||||
|---|---|---|---|---|---|---|---|---|
| CDDP | − | + | + | P4 | − | + | + | P4 |
| 4D3 | − | − | + | − | − | + | ||
| Necrotic area (%)1 | 2 ± 0.1 | 27 ± 3 | 48 ± 5 | < 0.001 | 14 ± 2 | 52 ± 7 | 68 ± 13 | < 0.001 |
| Ki-67 (%)2 | 88 ± 4 | 78 ± 3 | 75 ± 3 | < 0.05 | 92 ± 5 | 81 ± 4 | 76 ± 4 | < 0.05 |
| ss-DNA (%)2 | 12 ± 1 | 23 ± 2 | 34 ± 3 | < 0.05 | 8 ± 1 | 22 ± 1 | 34 ± 2 | < 0.05 |
| Cleaved caspase-3 (/HPF)3 | 1 ± 0.1 | 4 ± 0.1 | 6 ± 0.2 | < 0.001 | 2 ± 0.1 | 4 ± 0.1 | 10 ± 0.2 | < 0.001 |
1Necrotic area was evaluated in computer-captured images of the maximum cut surface of the tumors.
2Positivity was evaluated as positive by immunoreactivity in 20% or more nuclei of 1000 observed nuclei.
3Positive cells were counted by observation of 20 high power fields (HPFs).
4P value was calculated by ordinary ANOVA test.