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. 2018 Sep 8;525:1–9. doi: 10.1016/j.virol.2018.08.022

Fig. 2.

Fig. 2

FIPV Black-induced cell cytopathic effect varies between infected cell types. A) Progression of cell cytopathic effects (CPE) induced by FIPV Black over time in Fcwf-4 and AK-D cells (MOI=0.1). Localized cell syncytia formation (black, closed arrows) and discrete cell death (white, open arrows) indicated on brightfield images taken at 20x magnification. B) Immunofluorescence staining of FIPV Black-infected Fcwf-4 CU and AK-D cells (MOI=0.1) at indicated hours post-infection (hpi). Nucleocapsid protein staining (Red; CCV2-2) indicates infected cells; nuclei stained in blue (Hoesch3342). Images taken at 60x magnification.