Fig. 1.

A schematic diagram of the current study. All chicken fecal samples were analyzed to compare isolation methods of C. jejuni using culture-independent sequence-based metagenomics and culture-dependent tools. Metagenomic analysis was performed to investigate the effects of seven enrichment procedures. Six procedures involved enrichment processes (Bolton and Preston broth at 1:10¹, 1:10², and 1:10³, respectively), and one procedure did not. In culture-dependent analysis, a total of 14 procedures were applied for all samples, which were combinations of different enrichment broths (without enrichment process or enriched in Bolton broth or Preston broth), ratio of sample-to-enrichment broth (1:10¹, 1:10², or 1:10³), and selective media (mCCDA or Preston agar). ^aThe ratio of fecal sample-to-enrichment broth