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. 2019 Feb 28;9(6):1683–1697. doi: 10.7150/thno.30487

Figure 6.

Figure 6

Hematopoietic clusters could be found in the samples of in vivo bone formation. A. HE staining was used to observe the hematopoietic clusters and immunostaining with anti-CD45 antibody was applied for the detection of the nucleated cells of hematopoietic origin in in vivo transplants. More hematopoietic clusters and CD45+ cells were detected in the NMP-MSC group compared to the BMSC and control groups. Black arrows showed the location of hematopoietic cell clusters. HA/TCP, hydroxyl-apatite/tricalcium phosphate ceramic powder. Scale bar: 50 μm. B. Quantification of hematopoietic clusters (n=8) and CD45+ cells (n=6) were performed in different group. The data are expressed as mean±SEM. *p<0.05, **p<0.01, ***p<0.001, and n.s. is non-significant. C. The expression of hematopoietic supporting genes in cultured NMP-MSC and BMSC were detected by qRT-PCR. The data represent mean ± SEM of three independent experiments. *p<0.05, **p<0.01, ***p<0.001, and n.s. is non-significant.