Table 5. Comprehensive ranking order of top 10 reference genes obtained from 4 different methods used in this study.
| Ranking | ΔCt | geNorm | NormFinder | BestKeeper | Comprehensive ranking (mean rank value) |
|---|---|---|---|---|---|
| 1 | Rpl13a | Ywhaz/Rpl13a | Rpl13a | Rpl13a | Rpl13a (1.00) |
| 2 | Ywhaz | Rps18 | Actb | Ywhaz (2.50) | |
| 3 | Rps18 | Sdha | Rplp1 | Ywhaz | Rps18 (3.50) |
| 4 | Hmbs | Rps18 | Ywhaz | Sdha | Sdha (4.25) |
| 5 | Sdha | Hmbs | Sdha | Rps18 | Hmbs (5.50) |
| 6 | Ubc | Gapdh | Ubc | Hmbs | Rplp1 (6.00) |
| 7 | Rplp1 | Rplp1 | Hmbs | Rplp1 | Actb (7.00) |
| 8 | Gapdh | Ubc | Actb | Gapdh | Ubc (7.25) |
| 9 | Actb | Actb | Tbp* | Ubc | Hprt1 (7.50) |
| 10 | Hprt1 | Hprt1 | Gapdh | Hprt1 | Gapdh (8.00) |
Note:
*
Tbp was excluded from comprehensive ranking calculation because it appears only once in four analyses.