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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Nature. 2018 Oct 3;562(7728):595–599. doi: 10.1038/s41586-018-0581-5

Extended Data Figure 8. Otx2-/-; Blimp1-/- PGCLCs activate PGC markers.

Extended Data Figure 8

a. Quantitative analysis of somatic transcripts at day 2 of PGCLC induction in the indicated cell lines. Expression levels are normalised to TBP; Values are means ± SD; n=4 biological replicates, each dot represents the value from one experiment.

b. Quantitative analysis of PGC TF transcripts at day 2 of PGCLC induction in the indicated cell lines. Expression levels are normalised to TBP; Values are means ± SD; n=6 biological replicates for E14Tg2A and Otx2lazZ/GFP, and 4 for Blimp1 KO cell lines each dot represents the value from one experiment.

c. Immunofluorescence staining for OCT4 and DAZL of cryo-sections of Otx2lacZ/GFP and Otx2lacZ/GFP; Blimp1-/- aggregates at day6 of PGCLC induction. Bar; 50μm and 20μm. n= 2 biologically independent replicates.

d. OCT4, H3K27me3 and H3K9me2 immunofluorescence analysis of cryo-sections of E14Tg2a, Otx2lacZ/GFP and Otx2lacZ/GFP; Blimp1-/- aggregates at day6 of PGCLC induction. Bar; 50μm. n= 2 biologically independent replicates.