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. 2019 Apr 11;15:66–78. doi: 10.1016/j.isci.2019.04.011

Figure 3.

Figure 3

The Transportation of HLA-I in hESC-MSCs under IFN-γ Treatment

(A) HLA-I subcellular location in different organelles (including ER Golgi, endosome, and lysosome) of Day0, Day1, and Day3 hESC-MSCs. Red indicates organelles, green indicates HLA-I, and blue indicates DAPI. Scale bar, 50 μm.

(B) The immunofluorescence intensity analysis of HLA-I with different organelles in Day0, Day1, and Day3 hESC-MSCs (focused on the white line drawn on Figure 3A). The red line indicates organelles, and the green line indicates HLA-I.

(C) Mander's value analysis of co-localization rate of HLA-I and different organelles in Day1 and Day3 hESC-MSCs with the immunofluorescence results. n = 3 technical replicates. Data are shown as means ± SEM.

(D) HLA-I endocytosis analysis on hESC-MSCs with or without IFN-γ treatment by flow cytometry. The red line indicates IFN-γ-treated hESC-MSCs, and the blue line indicates IFN-γ-untreated hESC-MSCs; dashed line indicates the endocytosis rate of surface HLA-I.