Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-GFP (chicken polyclonal) |
Abcam | Abcam:ab13970; RRID:AB_300798 | ICC (1:1,000) |
| Antibody | anti-GFP (rat monoclonal; clone 3H9) | Chromotek | Chromotek:3h9-100; RRID:AB_10773374 | WB (1:2,000-1:5,000) |
| Antibody | anti-Ankyrin-G (mouse monoclonal; purified; clone N106/36) | NeuroMab | NeuroMab:75–146; RRID:AB_10673030 |
ICC (1:500) |
| Antibody | anti-CD4 (mouse monoclonal; purified; clone OKT4) | BioLegend | BioLegend:317402; RRID:AB_571963 | ICC (1:400) |
| Antibody | anti-MAP2 (rabbit polyclonal) | Synaptic Systems | SySy:188 003; RRID:AB_2281442 | ICC (1:500) |
| Antibody | anti-MAP Kinase, Activated/monophosphorylated, Phosphothreonine ERK-1 and 2 (mouse monoconal; clone ERK-PT115) |
Merck (Sigma-Aldrich) | Sigma-Aldrich:M7802; RRID:AB_260658 | WB (1:1,000) |
| Antibody | anti-MAP Kinase, Non-Phosphorylated ERK (mouse monoclonal; clone ERK-NP2) | Merck (Sigma-Aldrich) | Sigma-Aldrich:M3807; RRID:AB_260501 | WB (1:250) |
| Antibody | anti-GAPDH (mouse monoclonal; clone 6C5) | Abcam | Abcam:ab8245; RRID:AB_2107448 | WB (1:20,000) |
| Antibody (secondary) |
Peroxidase anti-mouse IgG (goat) | Merck (Sigma-Aldrich) | Sigma-Aldrich:A3682 | WB (1:10,000) |
| Antibody (secondary) |
Peroxidase anti-rat IgG (rabbit) | Merck (Sigma-Aldrich) | Sigma-Aldrich:A5795 | WB (1:10,000) |
| Antibody (secondary) |
Cy2 anti-chicken IgY (donkey) |
Jackson Immuno Research (Stratech) | JIR:703-225-155; RRID:AB_2340370 | ICC (1:400) |
| Antibody (secondary) |
Alexa Fluor 647 anti-chicken IgY (donkey) | Jackson Immuno Research (Stratech) | JIR:703-606-155; RRID:AB_2340380 | ICC (1:400) |
| Antibody (secondary) | Cy3 anti-chicken IgY (donkey) | Jackson Immuno Research (Stratech) | JIR:703-165-155; RRID:AB_2340363 | ICC (1:400) |
| Antibody (secondary) | Cy5 anti-mouse IgG (donkey) | Jackson Immuno Research (Stratech) | JIR:715-175-150; RRID:AB_2340819 | ICC (1:400) |
| Antibody (secondary) | Cy3 anti-mouse IgG (donkey) | Jackson Immuno Research (Stratech) | JIR:715-165-150; RRID:AB_2340813 | ICC (1:400) |
| Antibody (secondary) | DyLight 405 anti-mouse IgG (goat) | Jackson Immuno Research (Stratech) | JIR:115-475-003; RRID:AB_2338786 |
ICC (1:400) |
| Antibody (secondary) |
Alexa Fluor 488 anti-rabbit IgG (donkey) | Jackson Immuno Research (Stratech) | JIR:711-545-152; RRID:AB_2313584 | ICC (1:400) |
| Chemical compound, drug | Arachidonyl-2'-chloroethylamide (ACEA) | Bio-Techne (Tocris) | Tocris:1319 | |
| Chemical compound, drug | AM 281 | Bio-Techne (Tocris) | Tocris:1115 | Dissolved in DMSO |
| Cell line (H. sapiens) | HEK293T | ECACC (Sigma-Aldrich) | ECACC:12022001; RRID:CVCL_0063 | |
| Cell line (R. norvegicus) |
primary hippocampal neurons | University of Bristol Animal Services Unit | E18 Wistar Han rats | |
| Recombinant DNA reagent |
pIRESneo3_Str- KDEL_IRES-SPIl-2-SBP-mCherry-Ecadherin (plasmid) |
PMID:22406856 | Addgene:65287 | |
| Recombinant DNA reagent | pcDNA1-SPHgH-SEP-CB1RWT (plasmid) | PMID:17467290 | ||
| Recombinant DNA reagent | pcDNA1-SPHgH-SEP-CB1RΔH9 (plasmid) | This paper | PCR mutagenesis template: pcDNA1-SPHgH-SEP-CB1RWT | |
| Recombinant DNA reagent | pIRESneo3_Str-KDEL_IRES-SPIl-2-SBP-EGFP-CB1RWT (plasmid) | This paper | PCR template: pcDNA1-SPHgH-SEP-CB1R Vector: pIRESneo3_Str-KDEL_IRES-SPIl-2-SBP-mCherry- Ecadherin |
|
| Recombinant DNA reagent | pIRESneo3_Str-KDEL_IRES-SPIl-2-SBP-EGFP-CB1RΔH9 (plasmid) | This paper | PCR template: pcDNA1-SPHgH-SEP-CB1RΔH9
Vector: pIRESneo3_Str-KDEL_IRES-SPIl-2-SBP-mCherry-Ecadherin |
|
| Recombinant DNA reagent |
pcDNA3.1-SPIl-2-SBP-EGFP-CB1RWT (plasmid) | This paper | PCR template: pIRESneo3_Str-KDEL_IRES-SPIl-2-SBP-EGFP-CB1RWT
Vector: pcDNA3.1(+) |
|
| Recombinant DNA reagent | pcDNA3.1-SPIl-2-SBP-EGFP-CB1RΔH9 (plasmid) | This paper | PCR template: pIRESneo3_Str-KDEL_IRES-SPIl-2-SBP-EGFP-CB1RΔH9
Vector: pcDNA3.1(+) |
|
| Recombinant DNA reagent | pcDNA3.1-SPIl-2-EGFP-CB1RWT (plasmid) | This paper | PCR mutagenesis template: pcDNA3.1-SPIl-2-SBP-EGFP-CB1RWT | |
| Recombinant DNA reagent | pcDNA3.1-SPIl-2-EGFP-CB1RΔH9 (plasmid) | This paper | PCR mutagenesis template: pcDNA3.1-SPIl-2-SBP-EGFP-CB1RΔH9 | |
| Recombinant DNA reagent | pCB6-CD4ΔCt (plasmid) | PMID:11689435 | ||
| Recombinant DNA reagent |
pCB6-CD4-ctCB1RWT (plasmid) | This paper | Overlap extension PCR template: pcDNA1-SPHgH- SEP-CB1RWT Vector: pCB6-CD4ΔCt |
|
| Recombinant DNA reagent |
pCB6-CD4-ctCB1RΔH9 (plasmid) | This paper | Overlap extension PCR template: pcDNA1-SPHgH-SEP-CB1RΔH9
Vector: pCB6-CD4ΔCt |
|
| Chemical compound, drug | Neurobasal | Thermo Fisher Scientific (Gibco) | TFS:21103049 | |
| Chemical compound, drug | Horse Serum | Merck (Sigma-Aldrich) | Sigma-Aldrich:H1270 | |
| Chemical compound, drug | B27 | Thermo Fisher Scientific (Gibco) | TFS:A3582801 | |
| Chemical compound, drug |
GS21 | MTI GlobalStem | GS:3100 | |
| Chemical compound, drug |
GlutaMAX | Thermo Fisher Scientific (Gibco) | TFS:35050038 | |
| Chemical compound, drug | DMEM | Lonza | Lonza:12–614F | |
| Chemical compound, drug | L-Glutamine | Merck (Sigma-Aldrich) | Sigma-Aldrich:G7513 | |
| Chemical compound, drug | FBS | Merck (Sigma-Aldrich) | Sigma-Aldrich:F7524 | |
| Chemical compound, drug | Lipofectamine 2000 | Thermo Fisher Scientific | TFS:11668019 | |
| Chemical compound, drug | D-Biotin | Merck (Sigma-Aldrich) | Sigma-Aldrich:B4501 | |
| Chemical compound, drug | EZ-Link Sulfo-NHS-SS-Biotin | Thermo Fisher Scientific | TFS:21331 | |
| Chemical compound, drug | Streptavidin− Agarose |
Merck (Millipore) | Merck:S1638 | |
| Chemical compound, drug | Bovine Serum Albumin | Thermo Fisher Scientific | TFS:BP9704 | |
| Chemical compound, drug | Fluoromount-G | Thermo Fisher Scientific | TFS:00-4958-02 | |
| Chemical compound, drug | cOmplete Protease Inhibitor Cocktail | Merck (Sigma-Aldrich) | Sigma-Aldrich: 11697498001 |
|
| Chemical compound, drug | Pierce Phosphatase Inhibitor | Thermo Fisher Scientific | TFS:A32957 | |
| Chemical compound, drug | CHAPS Detergent | Thermo Fisher Scientific | TFS:28300 | |
| Software, algorithm | GraphPad Prism | GraphPad Prism (https://graphpad.com) | RRID:SCR_002798 | Version 7 |
| Software, algorithm | Fiji | Fiji (https://fiji.sc/) | RRID:SCR_002285 |