Table 2.

Stability of the PRIMVAC drug product at 25 °C with adjuvant.

TESTS	T0 + Alhydrogel®	4 h + Alhydrogel®	T0 + GLA-SE	4 h + GLA-SE
Visual appearence	White, opalescent w/o visible particles	White, opalescent w/o visible particles	Slightly brownish, opalescent w/o visible particles	Slightly brownish, opalescent w/o visible particles
pH	7.4	7.4	6.4	6.3
Profile by SDS-PAGE	Before desorption: no band	Before desorption: no band	One major band at 100 kDa. Few aggregates. Presence of GLA-SE materials and smears from 37 kDa	One major band at 100 kDa. Few aggregates. Presence of GLA-SE materials and smears from 37 kDa
	After desorption: One specific band at 100 kDa	After desorption: One specific band at 100 kDa
Identity by Western Blot (R and NR conditions)	Before desorption: no band	Before desorption: no band	One specific band at 100 kDa	One specific band at 100 kDa
	After desorption: One specific band at 100 kDa	After desorption: One specific band at 100 kDa
Protein content by BCA	Before desorption: ND	Before desorption: ND	398 μg/vial	398 μg/vial
	After desorption: 96 μg/vial	After desorption: 78 μg/vial