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. Author manuscript; available in PMC: 2019 Sep 13.
Published in final edited form as: Nature. 2019 Mar 13;567(7748):405–408. doi: 10.1038/s41586-019-1018-5

Figure 2 |. AmB improved host defenses in primary cultured airway epithelia derived from genetically diverse humans with CF.

Figure 2 |

(a) Effect of AmB on ASL pH on primary cultured airway epithelia derived from 9 humans with CF with different CFTR mutations (n = 9). (b) Average difference in ASL pH after AmB treatment as a function of time (t = 0, 0.5, 2 hours, n = 6; t = 6, 12, 24 hours, n = 3; t = 1, 48 hours, n = 9). Effect of apical AmB treatment on (c) ASL viscosity (τASL/τsaline) (n = 6) and (d) ASL antibacterial activity (n = 8) in primary CF epithelia. Graphs depict means ± SEM. Panels (a,b,d): each data point represents an average of 1-3 epithelia samples from each human donor. Panels (a,c,d): n are biologically independent samples from at least three independent experiments with similar results. Panel (b): the same samples for each donor were measured repeatedly over time and ** indicate differences versus vehicle; data is from at least three independent experiments with similar results. All panels: two-sided unpaired Student’s t test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. Exact P values in Source Data.