TABLE 1.
PfCRT and K13 haplotypes of recombinant isogenic parasite linesa
| Parasite line | Amino acid in PfCRT at the following position: |
Amino acid in K13 at position 539 |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| 74 | 75 | 76 | 220 | 271 | 326 | 356 | 371 | ||
| Dd23D7 (wild type) | M | N | K | A | Q | N | I | R | R |
| Dd2Dd2 | I | E | T | S | E | S | T | I | R |
| Dd2Cam783 (Dd2Dd2 S326N) | I | E | T | S | E | N | T | I | R |
| Dd2FCB (Dd2Dd2 T356I) | I | E | T | S | E | S | I | I | R |
| Dd2GB4 (Dd2Dd2 S326N T356I) | I | E | T | S | E | N | I | I | R |
| Dd2R539T3D7 | M | N | K | A | Q | N | I | R | T |
| Dd2R539TDd2 | I | E | T | S | E | S | T | I | T |
| Dd2R539TCam783 (Dd2R539TDd2 S326N) | I | E | T | S | E | N | T | I | T |
| Dd2R539TFCB (Dd2R539TDd2 T356I) | I | E | T | S | E | S | I | I | T |
| Dd2R539TGB4 (Dd2R539TDd2 S326N T356I) | I | E | T | S | E | N | I | I | T |
a
The mutational status of PfCRT (PlasmoDB PF3D7_0709000) and K13 (PF3D7_1343700) is summarized for all recombinant parasite lines generated in this study. Mutations are shown in boldface type. The PfCRT haplotype encoded by Dd2 (wild-type K13) and Dd2R539T (K13 R539T mutant) parasites is indicated in superscript, with 3D7 designating the wild-type PfCRT haplotype.