Abstract
Synchronization of mammalian cells by starvation‐refeeding or by inhibition‐release are among the most commonly used techniques for division cycle analysis. An alternative analysis—in the form of a Gedanken or thought experiment—is presented, casting doubt on the utility of this synchronization method. Arresting cell growth produces a culture where all cells contain a G1 amount of DNA. However, these cells are not arrested at a particular point in the G1‐phase. Analysis of ‘G1 arrested cells’ suggests that, upon resumption of growth, the cells are not synchronized.
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