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. Author manuscript; available in PMC: 2020 Apr 9.
Published in final edited form as: Biochemistry. 2019 Mar 27;58(14):1905–1917. doi: 10.1021/acs.biochem.8b01241

Figure 1. Interactions involving BH in SpyCas9.

Figure 1.

A) BH inserted into the nucleic acid interface B) Interactions of BH with the sgRNA seed region and the phosphate lock loop (PLL). Dashed lines represent interactions that are within 3.5 Å. C) Superposition of SpyCas9 BH from different crystal structures (apo-: PDB ID: 4CMP,29 binary: PDB ID: 4ZT0,28 ternary: PDB ID: 5F9R27). SL1- Stem loop 1. Figures were made using Pymol.42