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. Author manuscript; available in PMC: 2019 May 2.
Published in final edited form as: Leukemia. 2018 Oct 5;33(3):638–652. doi: 10.1038/s41375-018-0280-0

Figure 6. TNFα modulates transcription factor expression levels in BM HSPCs.

Figure 6.

(A-D) CD34+ cells from fresh, ACK-lysed HC BM were isolated and incubated in serum-free media supplemented with HSPC-supportive cytokines (see Methods). Cultures were additionally supplemented with TNFα (25ng/mL) or media only for 12 or 24 hours followed by intranuclear staining for (A-B) PU.1 (n=12, 9 individual experiments) or (C-D) GATA-2 (n=7, 6 individual experiments) among the HSC/MPP (CD34+CD38-CD45RA-) and lineage-biased (CD34+CD38+) compartments. All MFI values were subtracted against the isotype control and presented such that each point and connecting line indicates a paired sample. **P<0.01, ***P<0.001, and ****P<0.0001 were obtained by a paired t-test.