Figure 5.

Enhancer activity of D. willistoni yellow fragments. Below the schematic of overlapping sequence regions from the 5′ intergenic and intronic regions of the D. willistoni yellow gene tested for enhancer activity are images of transgenic pupae that show expression from the GFP reporter genes in magenta. Expression driven by fragments from the 5′ intergenic region (A and B) and the intron (C and D) is shown in the body (A and C) and wings (B and D), for both female (top row) and male (bottom row) flies. Summary tables below each pair of pupal bodies (A and C) and wings (B and D) show our interpretation of these images: “++” indicates strong fluorescence observed in the body region, whereas “+” indicates weaker fluorescence. Blue arrows highlight elevated expression in the proximal areas of the wing driven by the will_C2 fragment. The asterisk next to the will_C8 fragment indicates that activity of this element is shown for flies heterozygous for the reporter gene. All other images show GFP expression in flies homozygous for the reporter gene. The magenta color used in this figure makes it easier to see low expression levels; a copy of this figure with GFP expression shown in the more traditional green is provided as Figure S4. The wing blade expression driven by full will_5′ and will_C7 fragments were equivocal, and went through a secondary inspection before reaching a consensus among authors.