Figure 8. Behavior of homozygous frimousse (frm) larvae.

(a) VGAT and VACHT reporter construct expression in wild type (wt) and frm larvae. (b) Frequency of spontaneous swims of wt and frm larvae in dark conditions (i.e., 700 nm illumination). Each open circle represents one tracked larva with the number of swim bouts in one minute presented. Also shown are the averages (red circles) ±standard deviation. (c) Duration of all spontaneous swims in one-minute recording presented in seconds (s) for wt and frm larvae. (d) Standard deviation (S.D.) of the duration of swim bouts over one minute for each individual larva recorded. (e) S.D. of the interval between swim bouts over one minute for each individual larva recorded. For d and e, larvae with <4 swim bouts were not included in the analysis. ***, p<0.001; n.s., not significant.