Skip to main content
. 2019 Apr 10;8:e45239. doi: 10.7554/eLife.45239

Figure 6. Examples of sorted multinucleated H226 cells (A, C) 1 and 2 (B, D) days after scMOCa.

Active mitochondria (Mitotracker) appear in green, plasma membrane (WGA-Alexa 647) in red, an nuclei (Hoechst) in white. Scale bar: 15 µm. (E) 3T3 cell population partially differentiated into adipocytes. Two cells (pointed by arrows) have been tagged with magnetic beads. Three other cells are also differentiated in adipocytes in this field of view but were not selected. (F) Cells were captured using scMOCA and kept in culture for 6 days before imaging. Cells were stained with WGA-Alexa 647 to highlight membranes. Small black circles are magnetic beads while lipid vesicles appear as small clear circles. Scale bar: 30 µm.

Figure 6.

Figure 6—figure supplement 1. Two examples of cells that were killed with sodium azide then stained with mitotracker green and Hoechst.

Figure 6—figure supplement 1.

This negative control confirms the cells shown in Figure 6 are live cells. When cells are alive, mitotracker green stains filament shaped mitochondria, whereas when cells are dead, the spotty signal it generates is distributed everywhere within the cytoplasm.