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. 2018 Oct 12;68(7):1259–1270. doi: 10.1136/gutjnl-2017-315490

Figure 1.

Figure 1

Conditional deletion of Arid1a in the pancreas induces inflammation, pancreatic intraepithelial neoplasia (PanIN) and mucinous cysts. (A) In wild-type mice, ARID1A was expressed in most acinar cells (red arrowheads), all duct cells (‘D’ adjacent) and all islet cells (asterisk). In Ptf1a-Cre; Arid1a f/f (CA) mice, ARID1A was lost in all acinar cells (yellow arrowheads), some duct cells (red arrows point to lost, red arrowheads point to retained) and none of the islet cells. ­­ Sixteen-week-old CA mice formed (B) PanIN and (C) large cysts with flat to cuboidal epithelium with papillary projections (arrows) that were Alcian blue and claudin-18 positive. Both PanIN and cysts showed focal ARID1A positivity (arrowheads point to ARID1A-positive cells). (D) The cysts expressed embryonic factors PDX1 and HNF4a. (E) The pancreata of CA mice showed significant upregulation of chemokines and cytokines, as measured by quantitative PCR, n≥5, *P<0.05, **P<0.01.