Fig. 3.

Dose-dependent effect of DWK-1 and Co DWK-1 on the accumulation of intracellular ZIKV RNA in infected human podocytes. Podocytes were pretreated for 24 h with the indicated doses of DWK-1 or Co DWK-1, rinsed and infected with ZIKV at MOI of 0.1 in the absence of morpholinos. Total RNA was isolated from the mock infected and ZIKV-infected cells at 72 h p.i. Expression of ZIKV RNA was determined by qRT-PCR and normalized to GAPDH mRNA expression. ZIKV infections were performed in triplicate. Values represent mean ± SD of 3 independent samples. *P < 0.001. ND, not detected.