Skip to main content
NCBI home page
Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1990 Jun-Jul;81(6-7):632–638. doi: 10.1111/j.1349-7006.1990.tb02620.x

Loss of Heterozygosity on Chromosomes 1 and 11 in Sporadic Pheochromocytomas

Yutaka Yokogoshi 1, Katsuhiko Yoshimoto 1, Shiro Saito 1
PMCID: PMC6504067  PMID: 1976119

Abstract

Molecular genetic analysis was performed with 20 oncogene probes and 32 polymorphic DNA probes on tumor DNA samples from seven pheochromocytomas; namely, one multiple endocrine neoplasia type 2B, and two familial and four sporadic pheochromocytomas. No amplification or rearrangement of the oncogenes was detected in any of the tumors. However, loss of heterozygosity on chromosome 1p, 11p or 11q was detected in these cases. In addition, a locus related to ETS1 was deleted in two of the sporadic tumors. These results suggest that pheochromocytomas may be genetically heterogeneous, and that inactivation of unknown genes on chromosome 1p, 11p or 11q may contribute to their development.

Keywords: Pheochromocytoma, Loss of heterozygosity, Oncogene, Chromosome 11

Full Text

The Full Text of this article is available as a PDF (595.5 KB).

REFERENCES

  • 1. ) Sammaan , N. A. and Hickey , R. C. Pheochromocytoma . Semin. Oncol. , 14 , 297 – 305 ( 1987. ). [PubMed] [Google Scholar]
  • 2. ) Klein , G. The approaching era of the tumor suppressor genes . Science , 238 , 1539 – 1545 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 3. ) Nishimura , S. and Sekiya , T. Human cancer and cellular oncogenes . Biochem. J. , 243 , 313 – 327 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4. ) Knudson , A. G. , Meadows , A. T. , Nichols , W. W. and Hill , R. Chromosomal deletion and retinoblastoma . N. Engl. J. Med. , 295 , 1120 – 1123 ( 1976. ). [DOI] [PubMed] [Google Scholar]
  • 5. ) Brodeur , G. M. , Green , A. A. , Hayes , F. A. , Williams , K. J. , Williams , D. L. and Tsiatis , A. A. Cytogenetic features of human neuroblastomas and cell lines . Cancer Res. , 41 , 4678 – 4686 ( 1981. ). [PubMed] [Google Scholar]
  • 6. ) Kaneko , Y. , Egues , M. C. and Rowley , J. D. Interstitial deletion of short arm of chromosome 11 limited to Wilms' tumor cells in a patient without aniridia . Cancer Res. , 41 , 4577 – 4578 ( 1981. ). [PubMed] [Google Scholar]
  • 7. ) Koufos , A. , Hansen , M. F. , Copeland , N. G. , Jenkins , N. A. , Lampkin , B. C. and Cavenee , W. K. Loss of heterozygosity in three embryonal tumours suggests a common pathogenetic mechanism . Nature , 316 , 330 – 334 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 8. ) Ponder , B. Gene losses in human tumours . Nature , 335 , 400 – 402 ( 1988. ). [DOI] [PubMed] [Google Scholar]
  • 9. ) Ohno , F. , Yamano , T. and Kataoka , K. A case of congenital aniridia and familial pheochromocytoma — with special reference to aniridia Wilms' tumor syndrome . Jpn. J. Hum. Genet. , 27 , 335 – 340 ( 1982. ). [DOI] [PubMed] [Google Scholar]
  • 10. ) Yoshimoto , K. , Iizuka , M. , Iwahana , H. , Yamasaki , R. , Saito , H. , Saito , S. and Sekiya , T. Loss of the same alleles of HRAS1 and D11S151 in two independent pancreatic cancers from a patient with multiple endocrine neoplasia type 1 . Cancer Res. , 49 , 2716 – 2721 ( 1989. ). [PubMed] [Google Scholar]
  • 11. ) Blin , N. and Stafford , D. W. A general method for isolation of high molecular weight DNA from eukaryotes . Nucleic Acids Res. , 3 , 2303 – 2308 ( 1976. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12. ) Pearson , P. L. , Kidd , K. K. and Willard , H. F. Report of the Committee on Human Gene Mapping by Recombinant DNA Techniques . Cytogenet. Cell Genet. , 46 , 390 – 566 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 13. ) Feinberg , A. P. and Vogelstein , B. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity . Anal. Biochem. , 132 , 6 – 13 ( 1983. ). [DOI] [PubMed] [Google Scholar]
  • 14. ) Southern , E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis . J. Mol. Biol. , 98 , 503 – 517 ( 1975. ). [DOI] [PubMed] [Google Scholar]
  • 15. ) Jackson , C. E. , Conneally , P. M. , Sizemore , G. W. and Tashjian , A. H. , Jr. Possible linear order of genes for endocrine neoplasia type 2, the P red cell antigen and HLA on chromosome 6 . In “ Cancer and Genetics, Birth Defects ,” ed. Bergsman D. , Original Article Series, Vol. 12, No. 1 , pp. 159 – 164 ( 1976. ). Alan R. Liss; , New York . [PubMed] [Google Scholar]
  • 16. ) Cadotte , M. , Verdy , M. and Lacroix , A. A family with a 16qh+ chromosome and multiple endocrine neoplasia type 2 . Hum. Pathol. , 13 , 1056 ( 1982. ). [DOI] [PubMed] [Google Scholar]
  • 17. ) Van‐Dyke , D. L. , Jakson , C. E. and Babu , V. R. Multiple endocrine neoplasia type 2 (MEN‐2): an autosomal dominant syndrome with a possible chromosome 20 deletion . Am. J. Hum. Genet. , 33 , 64A ( 1981. ). [Google Scholar]
  • 18. ) Wurster‐Hill , D. H. , Noll , W. W. , Bircher , L. Y. , Pettengill , O. S. and Grizzle , W. A. Cytogenetics of medullary carcinoma of the thyroid . Cancer Genet. Cytogenet. , 20 , 247 – 253 ( 1986. ). [DOI] [PubMed] [Google Scholar]
  • 19. ) Simpson , N. E. , Kidd , K. K. , Goodfellow , P. J. , McDermid , H. , Myers , S. , Kidd , J. R. , Jackson , C. E. , Duncan , A. M. V. , Farrer , L. A. , Brasch , K. , Castiglione , C. , Genel , M. , Gertner , J. , Greenberg , C. R. , Gusella , J. F. , Holden , J. J. A. and White , B. N. Assignment of multiple endocrine neoplasia type 2A to chromosome 10 by linkage . Nature , 328 , 528 – 530 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 20. ) Mathew , C. G. P. , Chin , K. S. , Easton , D. F. , Thorpe , K. , Carter , C. , Liou , G. I. , Fong , S‐L. , Bridges , C. D. B. , Haak , H. , Nieuwenhuijzen Kruseman , A. C. , Schifter , S. , Hansen , H. H. , Telenius , H. , Telenius‐Berg , M. and Ponder , B. A. J. A linked genetic marker for multiple endocrine neoplasia type 2A on chromosome 10 . Nature , 328 , 527 – 528 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 21. ) Yamamoto , M. , Takai , S. , Miki , T. , Motomura , K. , Okazaki , M. , Nishisho , I. , Tateishi , H. , Miyauchi , A. , Honjo , T. Pakstis , A. J. and Mori , T. Close linkage of MEN2A with RBP3 locus in Japanese kindreds . Hum. Genet. , 82 , 287 – 288 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 22. ) Mathew , C. G. P. , Smith , B. A. , Thorpe , K. , Wong , Z. , Royle , N. J. , Jeffreys , A. J. and Ponder , B. A. J. Deletion of genes on chromosome 1 in endocrine neoplasia . Nature , 328 , 524 – 526 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 23. ) Watson , D. K. , McWilliams‐Smith , M. J. , Kozak , C. , Reeves , R. , Gearhart , J. , Nunn , M. F. , Nash , W. , Fowle , J. R. , III , Duesberg , P. , Papas , T. S. and O'Brien , S. J. Conserved chromosomal positions of dual domains of the ets protooncogene in cats, mice and humans . Proc. Natl. Acad. Sci. USA , 83 , 1792 – 1796 ( 1986. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24. ) Reddy , E. S. P. , Rao , V. N. and Papas , T. S. The erg gene: a human gene related to the ets oncogene . Proc. Natl. Acad. Sci. USA , 84 , 6131 – 6135 ( 1987. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25. ) Rao , V. N. , Huebner , K. , Isobe , M. , Ar‐Rushdi , A. , Croce , C. M. and Reddy , E. S. P. elk, tissue‐specific ets‐related genes on chromosomes × and 14 near translocation break points . Science , 244 , 66 – 70 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 26. ) Tsutsumi , M. , Yokota , J. , Kakizoe , T. , Koiso , K. , Sugimura , T. and Terada , M. Loss of heterozygosity on chromosomes 1p and 11p in sporadic pheochromocytoma . J. Natl. Cancer Inst. , 81 , 367 – 370 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 27. ) Fong , C‐T. , Dracopoli , N. C. , White , P. S. , Merrill , P. T. , Griffith , R. C. , Housman , D. E. and Brodeur , G. M. Loss of heterozygosity for the short arm of chromosome 1 in human neuroblastomas: correlation with N‐myc amplification . Proc. Natl. Acad. Sci. USA , 86 , 3753 – 3757 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28. ) Weissman , B. E. , Saxon , P. J. , Pasquale , S. R. , Jones , G. R. , Geiser , A. G. and Stanbridge , E. J. Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression . Science , 236 , 175 – 180 ( 1987. ). [DOI] [PubMed] [Google Scholar]
  • 29. ) Bouck , N. , Stoler , A. and Polverini , P. J. Coordinate control of anchorage independence, actin cytoskeleton, and angiogenesis by human chromosome 1 in hamster‐human hybrids . Cancer Res. , 46 , 5101 – 5105 ( 1986. ). [PubMed] [Google Scholar]

Articles from Japanese Journal of Cancer Research : Gann are provided here courtesy of Wiley

RESOURCES